Conessine Treatment Reduces Dexamethasone-Induced Muscle Atrophy by Regulating MuRF1 and Atrogin-1 Expression
Abstract
Conessine, a steroidal alkaloid, is a potent histamine H3 antagonist with anti-malarial activity. We recently reported that conessine treatment interferes with H₂O₂-induced cell death by regulating autophagy. However, the cellular signaling pathways involved in conessine treatment are not fully understood. Here, we report that conessine reduces muscle atrophy by interfering with the expression of atrophy-related ubiquitin ligases MuRF-1 and atrogin-1. Promoter reporter assay revealed that conessine treatment inhibits FoxO3a-dependent transcription, NF-kappaB-dependent transcription and p53-dependent transcription. We also showed that conessine treatment reduced dexamethasone-induced expression of MuRF1 and atrogin-1 by the quantitative RT-PCR and Western blot. Finally, we demonstrated that conessine treatment reduced dexamethasone-induced muscle atrophy using differentiated C2C12 cells. These results collectively suggest that conessine is potentially useful in the treatment of muscle atrophy.
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Conessine reduces dexamethasone-induced muscle atrophy by downregulating the expression of MuRF1 and MAFbx in C2C12 myotubes.
“Conessine, a steroidal alkaloid, reduces dexamethasone-induced muscle atrophy by downregulating the expression of MuRF1 and MAFbx in C2C12 myotubes [138].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [138]
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“A growing body of evidence has shown that the prolonged use of Dex leads to muscle atrophy in mice [19,20].”
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RetractedLee, M.-H., Lee, J.-H., Kim, W.-J., Kim, S. H., Kim, S., Kim, H. S., & Kim, T. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23094778 · Cited by 14 · see citing works
“In addition, transcriptional regulation of Atrogin-1/MAFbx mRNA is induced during muscle atrophy, similar to MuRF1 [46].”
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“FOXO3a, as the major transcription factor of MuRF-1 and Atrogin-1, is closely related to the development of atrophying muscle [14–16].”
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“Conessine has been reported to have various pharmacological effects, such as anti-plasmoidal [7], anti-amebiasis [8,9], anti-tumor [10], anti-muscular atrophy [11], and anti-acetylcholinesterase [12].”
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“…(Jewboonchu et al., 2022; Siriyong et al., 2016; Siriyong et al., 2017; Siriyong et al., 2018), anti-plasmodial (Dua et al., 2013), anti-inflammatory and anti-muscular atrophy (Kim et al., 2018; Kim et al., 2016), anti-tumor (Li et al., 2020) and anti-acetylcholinesterase (Yang et al., 2012).”
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Glabridin Inhibits Dexamethasone-Induced Muscle Atrophy
Yoshioka, Y., Kubota, Y., Samukawa, Y., Yamashita, Y., & Ashida, H. (2019) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2019.02.006 · Cited by 54 · see citing works
- Authors
- Kim, H., Jang, M., Park, R., Jo, D., Choi, I., Choe, J., Oh, W. K., & Park, J.
- Affiliations
- Yonsei University (KR)
- Korea Advanced Institute of Science and Technology (KR)
- Seoul National University (KR)
- Venue
- Journal of Microbiology and Biotechnology
- Published
- 2018-04-28
- Volume
- 28
- Issue
- 4
- Pages
- 520-526
- ISSN
- 1017-7825 (ISSN-L)
- 1738-8872
- OpenAlex
- W2887506778
- Cited by
- 23
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 5 · 2019: 3 · 2018: 1
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- conessine, muscle atrophy, dexamethasone, MuRF1, atrogin-1, ubiquitin ligase, FOXO3, NF-κB, p53, transcriptional regulation, C2C12 myoblasts
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