The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
MicroRNA-21 Is Up-Regulated in Allergic Airway Inflammation and Regulates IL-12p35 Expression
Open accessAbstract
Allergic airway inflammation is characterized by marked in situ changes in gene and protein expression, yet the role of microRNAs (miRNAs), a new family of key mRNA regulatory molecules, in this process has not yet been reported. Using a highly sensitive microarray-based approach, we identified 21 miRNAs with differential expression between doxycycline-induced lung-specific IL-13 transgenic mice (with allergic airway inflammation) and control mice. In particular, we observed overexpression of miR-21 and underexpression of miR-1 in the induced IL-13 transgenic mice compared with control mice. These findings were validated in two independent models of allergen-induced allergic airway inflammation and in IL-4 lung transgenic mice. Although IL-13-induced miR-21 expression was IL-13Rα1 dependent, allergen-induced miR-21 expression was mediated mainly independent of IL-13Rα1 and STAT6. Notably, predictive algorithms identified potential direct miR-21 targets among IL-13-regulated lung transcripts, such as IL-12p35 mRNA, which was decreased in IL-13 transgenic mice. Introduction of pre-miR-21 dose dependently inhibited cellular expression of a reporter vector harboring the 3′-untranslated region of IL-12p35. Moreover, mutating miR-21 binding sites in IL-12p35 3′-untranslated region abrogated miR-21-mediated repression. In summary, we have identified a miRNA signature in allergic airway inflammation, which includes miR-21 that modulates IL-12, a molecule germane to Th cell polarization.
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Cites
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Combinatorial microRNA target predictions
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“Although some of the targets of miRNAs are modulated through translational inhibition only, the majority of targets have mRNA level changes inverse to their respective miRNA regulator (49, 50).”
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MicroRNA 21 Promotes Glioma Invasion by Targeting Matrix Metalloproteinase Regulators
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BackgroundMicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene
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MicroRNA expression in the adult mouse central nervous system
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MicroRNA-21 targets Sprouty2 and promotes cellular outgrowths
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Cited by
Physiological and pathological roles for microRNAs in the immune system
O'Connell, R. M., Rao, D. S., Chaudhuri, A. A., & Baltimore, D. (2010) · Nature Reviews Immunology · doi:10.1038/nri2708 · Cited by 1,378
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Inflammation and cancer: interweaving microRNA, free radical, cytokine and p53 pathways
Schetter, A. J., Heegaard, N. H. H., & Harris, C. C. (2010) · Carcinogenesis · doi:10.1093/carcin/bgp272 · Cited by 657
Regulation and function of miRNA-21 in health and disease
Kumarswamy, R., Volkmann, I., & Thum, T. (2011) · RNA Biology · doi:10.4161/rna.8.5.16154 · Cited by 607
Pan, W., Zhu, S., Yuan, M., Cui, H., Wang, L., Luo, X., Li, J., Zhou, H., Tang, Y., & Shen, N. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0904060 · Cited by 561
Metadata
- Authors
- Lu, T. X., Munitz, A., & Rothenberg, M. E.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-04
- Volume
- 182
- Issue
- 8
- Pages
- 4994-5002
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1526700155
- Cited by
- 570
- References
- 12
- FWCI
- 24.41
- Type
- journal article
- Language
- en
- Open access
- bronze
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