The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Expression of Helios, an Ikaros Transcription Factor Family Member, Differentiates Thymic-Derived from Peripherally Induced Foxp3+ T Regulatory Cells
Open accessAbstract
Helios, a member of the Ikaros transcription factor family, is preferentially expressed at the mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using a newly generated mAb and demonstrated that it is expressed in all thymocytes at the double negative 2 stage of thymic development. Although Helios was expressed by 100% of CD4+CD8−Foxp3+ thymocytes, its expression in peripheral lymphoid tissues was restricted to a subpopulation (∼70%) of Foxp3+ T cells in mice and humans. Neither mouse nor human naive T cells induced to express Foxp3 in vitro by TCR stimulation in the presence of TGF-β expressed Helios. Ag-specific Foxp3+ T cells induced in vivo by Ag feeding also failed to express Helios. Collectively, these results demonstrate that Helios is potentially a specific marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3+ Treg cells are generated extrathymically.
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“In mice, high expression of Helios and low expression of Neuropilin-1 (Nrp-1) has been proposed to discriminate between thymus Treg and peripherally-induced Tregs [364, 365]”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [364]
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“Yet, Helios, Nrp-1 or ROR-γt are widely accepted for the discrimination of tTreg and pTreg cells under homeostatic conditions [364-366, 437-440].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [364]
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Wildin, R. S., Ramsdell, F., Peake, J., Faravelli, F., Casanova, J., Buist, N. R. M., Levy‐Lahad, E., Mazzella, M., Goulet, O., Perroni, L., et al. (2001) · Nature Genetics · doi:10.1038/83707 · Cited by 1,916
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Self-citationMechanisms of Foxp3+ T Regulatory Cell-Mediated Suppression
Shevach, E. M. (2009) · Immunity · doi:10.1016/j.immuni.2009.04.010 · Cited by 1,787
Self-citationZhou, X., Bailey-Bucktrout, S. L., Jeker, L. T., Peñaranda, C., Martínez‐Llordella, M., Ashby, M., Nakayama, M., Rosenthal, W. L., & Bluestone, J. A. (2009) · Nature Immunology · doi:10.1038/ni.1774 · Cited by 1,374
Inducing and expanding regulatory T cell populations by foreign antigen
Kretschmer, K., Apostolou, I., Hawiger, D., Khazaie, K., Nussenzweig, M. C., & von Boehmer, H. (2005) · Nature Immunology · doi:10.1038/ni1265 · Cited by 1,240
Molecular Antagonism and Plasticity of Regulatory and Inflammatory T Cell Programs
Yang, X. O., Nurieva, R., Martínez, G., Kang, H. S., Chung, Y., Pappu, B. P., Shah, B., Chang, S. H., Schluns, K. S., Watowich, S. S., et al. (2008) · Immunity · doi:10.1016/j.immuni.2008.05.007 · Cited by 1,169
Foxp3-dependent programme of regulatory T-cell differentiation
Gavin, M. A., Rasmussen, J. P., Fontenot, J. D., Vasta, V., Manganiello, V. C., Beavo, J. A., & Rudensky, A. Y. (2007) · Nature · doi:10.1038/nature05543 · Cited by 1,127
Natural and Adaptive Foxp3+ Regulatory T Cells: More of the Same or a Division of Labor?
de Lafaille, M. A. C., & Lafaille, J. J. (2009) · Immunity · doi:10.1016/j.immuni.2009.05.002 · Cited by 1,055
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The Microbial Metabolites, Short-Chain Fatty Acids, Regulate Colonic T reg Cell Homeostasis
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Induction of Colonic Regulatory T Cells by Indigenous Clostridium Species
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Metadata
- Authors
- Thornton, A. M., Korty, P. E., Tran, D. Q., Wohlfert, E. A., Murray, P. E., Belkaid, Y., & Shevach, E. M.
- Affiliations
- National Institutes of Health (US)
- Laboratory of Immunology
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-04-01
- Volume
- 184
- Issue
- 7
- Pages
- 3433-3441
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- PMC
- PMC3725574
- OpenAlex
- W2111329034
- Cited by
- 1,382
- References
- 47
- Citations by year
- 2026: 44 · 2025: 55 · 2024: 63 · 2023: 50 · 2022: 70 · 2021: 57 · 2020: 70 · 2019: 84 · 2018: 82 · 2017: 74 · 2016: 97 · 2015: 119 · 2014: 110 · 2013: 144 · 2012: 129
- FWCI
- 55.71
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › FOXO transcription factor regulation
- Keywords
- Helios, Foxp3+ regulatory T cells, T cell development, double-negative thymocytes, TGF-beta
- MeSH terms
- Animals, Cell Differentiation, Cell Separation, DNA-Binding Proteins, Female, Flow Cytometry, Humans, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Spleen, Thymus Gland, Transcription Factors, T-Lymphocyte Subsets, Cell Lineage, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Regulatory, Mice, Ikaros Transcription Factor, Forkhead Transcription Factors
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