The DOI matches its Crossref record.
Enzyme-Linked Immunosorbent Assay, Elisa
Abstract
A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after washing provides a measure of the amount of specific antibodies in the serum. Coating of polystyrene tubes with antigen is described, as well as the preparation of specifically purified antibodies against rabbit IgG, and their conjugation to alkaline phosphatase. When rabbit antisera against human serum albumin or against the dinitrophenyl group (DNP) were incubated in tubes coated with antigen, less than 1 ng/ml of specific antibody could be detected in both systems. Antibodies in unknown sera could be quantitated by comparison with a standard antiserum. The results obtained compared well with those obtained with other methods for antibody determination. In the DNP system, the specificity of the reaction was assessed by inhibition with hapten. The reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by hapten in solution.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Strickland, D. K., Ashcom, J. D., Williams, S., Burgess, W. H., Migliorini, M., & Argraves, W. S. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)38172-9 · Cited by 558
Participation in Normal Immune Responses of a Metastasis-Inducing Splice Variant of CD44
Arch, R., Wirth, K., Hofmann, M., Ponta, H., Matzku, S., Herrlich, P., & Zöller, M. (1992) · Science · doi:10.1126/science.1496383 · Cited by 403
Purification and characterization of thin, aggregative fimbriae from Salmonella enteritidis
Collinson, S. K., Emödy, L., Müller, K. H., Trust, T. J., & Kay, W. W. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.15.4773-4781.1991 · Cited by 346
Structure and antigenicity of the major specific glycolipid antigen of Mycobacterium leprae.
Hunter, S. W., Fujiwara, T., & Brennan, P. J. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33395-7 · Cited by 268
Application of immunochemical assays to food analysis
Gazzaz, S. S., Rasco, B. A., & Dong, F. M. (1992) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408399209527597 · Cited by 32
Metadata
- Authors
- Engvall, E., & Perlmann, P.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1972-07-01
- Volume
- 109
- Issue
- 1
- Pages
- 129-135
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W4313315460
- Cited by
- 1,757
- References
- 0
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Enzyme-Linked Immunosorbent Assay, Elisa. https://app.cochat.ai/papers/10.4049/jimmunol.109.1.129
- The paper
- Engvall, E., & Perlmann, P. (1972). Enzyme-Linked Immunosorbent Assay, Elisa. The Journal of Immunology. https://doi.org/10.4049/jimmunol.109.1.129
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
