The DOI matches its Crossref record.
Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta.
Abstract
Human peripheral blood mononuclear cells (PBMC) were induced by recombinant interleukin 2 and mitogens to secrete two distinct cytotoxic polypeptides, tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin. Treatment of PBMC with recombinant human interleukin 2 (rIL 2) or mitogens in combination with recombinant human interferon-gamma (rIFN-gamma) resulted in augmented production of both TNF-alpha and TNF-beta. rIFN-gamma alone had no effect on production of either cytotoxic polypeptide. TNF-alpha was produced within 2 to 3 hr after induction and was the major cytotoxin produced by PBMC during the first 48 hr of culture, after which time TNF-beta became the predominant species. TNF-beta was first secreted into the media after 8 hr of induction. Enhanced levels of both TNF-alpha and TNF-beta were seen when the PBMC were separated into adherent and nonadherent cells. Both TNF-alpha and TNF-beta were induced in different tumor cell lines of hematopoietic origin. The results demonstrate that the production of TNF-alpha and TNF-beta can be enhanced by two lymphokines, IL 2 and IFN-gamma.
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Citations
Cited by
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Dinarello, C. A., Cannon, J. G., Wolff, S. M., Bernheim, H. A., Beutler, B., Cerami, A., Figari, I. S., Palladino, M. A., & O'Connor, J. V. (1986) · The Journal of experimental medicine · doi:10.1084/jem.163.6.1433 · Cited by 1,472
Self-citationRanges, G. E., Figari, I. S., Espevik, T., & Palladino, M. A. (1987) · The Journal of experimental medicine · doi:10.1084/jem.166.4.991 · Cited by 494
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Metadata
- Authors
- Nedwin, G. E., Svedersky, L. P., Bringman, T. S., Palladino, M. A., & Goeddel, D. V.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1985-10-01
- Volume
- 135
- Issue
- 4
- Pages
- 2492-2497
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2131632510
- Cited by
- 485
- References
- 0
- FWCI
- 19.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nedwin, G. E., Svedersky, L. P., Bringman, T. S., Palladino, M. A., & Goeddel, D. V. (1985). Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta. The Journal of Immunology. https://doi.org/10.4049/jimmunol.135.4.2492
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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