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A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha -chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions.
Open accessAbstract
In the present study a unique antibody (NKI-L16) reacting with the alpha-chain of the human leukocyte function-associated Ag-1 (LFA-1) is described, which stimulates homotypic cell-cell interactions in a manner very similar to 12-O-tetradecanoyl-phorbol-13-acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. The induction of aggregate formation of EBV-transformed B cells (JY) and CTL clones by TPA or NKI-L16 is not accompanied by an increase in the expression of LFA-1. Nevertheless, this cluster formation is LFA-1 dependent, inasmuch as anti-LFA-1 antibodies, other than NKI-L16, completely abrogate aggregation. Simultaneous addition of NKI-L16 and TPA did not result in a further increase of the speed of cluster formation, suggesting that a similar pathway is activated. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the alpha-chain of LFA-1, most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuration, thereby permitting binding of LFA-1 to its natural ligand.
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Citations
Cited by
Adhesion receptors of the immune system
Springer, T. A. (1990) · Nature · doi:10.1038/346425a0 · Cited by 6,477
T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1
Dustin, M. L., & Springer, T. A. (1989) · Nature · doi:10.1038/341619a0 · Cited by 1,538
Luque, A., Gómez, M., Puzon, W., Takada, Y., Sánchez-Madrid, F., & Cabañas, C. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.19.11067 · Cited by 296
Metadata
- Authors
- Keizer, G. D., Visser, W., Vliem, M., & Figdor, C. G.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1988-03-01
- Volume
- 140
- Issue
- 5
- Pages
- 1393-1400
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1575164560
- Cited by
- 241
- References
- 0
- FWCI
- 5.36
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha -chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions. https://app.cochat.ai/papers/10.4049/jimmunol.140.5.1393
- The paper
- Keizer, G. D., Visser, W., Vliem, M., & Figdor, C. G. (1988). A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha -chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions. The Journal of Immunology. https://doi.org/10.4049/jimmunol.140.5.1393
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