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Cited by 114Immunotherapy and Immune ResponsesT-cell and B-cell ImmunologyCancer Immunotherapy and Biomarkers

B7-1 but not B7-2 efficiently costimulates CD8+ T lymphocytes in the P815 tumor system in vitro

Gajewski, T F·1996·The Journal of Immunology
DOI 10.4049/jimmunol.156.2.465
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Abstract

We recently have developed a method to generate primary P815-specific CTL in vitro from normal syngeneic splenocytes by employing transfection of B7-1 combined with exogenous IL-12 and IL-6. Surprisingly, when the homologous costimulator molecule B7-2 was substituted for B7-1 in this system, no specific CTL activity was obtained. Similarly, B7-1- but not B7-2-transfected P815 cells generated alloantigen-specific CTL activity from C57BL/6, accessory cell- and CD4(+)-depleted splenocytes, and costimulated proliferation of CD8+ lymphocytes in the presence of low doses of anti-CD3 mAb. In all systems, combined expression of both B7-1 and B7-2 costimulated as effectively as B7-1 alone, arguing against delivery of a dominant negative signal by B7-2. Proliferation of allogeneic, CD4(+)-depleted splenocytes in response to P815 cells, which relies on costimulation by normal accessory cells, was inhibited by anti-B7-1 but not anti-B7-2 mAbs. Finally, indirect evidence suggested a higher avidity of B7-1+ cells than B7-2+ cells for CTLA4. Thus, at least in the context of primary stimulation by irradiated P815 transfectants, B7-1 appears to be superior to B7-2 at costimulation of CD8+ T lymphocytes.

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Crossref · 10.4049/jimmunol.156.2.465

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  • In animal models, introducing B7-1 into tumors or blocking inhibitory B7 family members reduces tumor growth or causes spontaneous tumor rejection.

    “Animal model studies have shown that introduction of B7-1 into tumors by transfection or blockade of inhibitory B7 family members can reduce tumor growth or result in spontaneous tumor rejection in vivo [7–13].”

    From T cell anergy, exhaustion, senescence, and stemness in the tumor microenvironment (2013) · cites this paper as [12]

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Authors
Gajewski, T. F.
Affiliations
Ludwig Cancer Research (BE)
Corresponding author
Gajewski, T F
ORCID
Gajewski, T F
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
1996-01
Volume
156
Issue
2
Pages
465-472
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.156.2.465
OpenAlex
W1946324007
Cited by
114
References
0
Citations by year
2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 1 · 2012: 2
FWCI
7.40
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Digital Access to Libraries (Université catholique de Louvain (UCL), l'Université de Namur (UNamur) and the Université Saint-Louis (USL-B)) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Health Sciences › Medicine › Oncology › Cancer Immunotherapy and Biomarkers
Keywords
CD80, CD86, costimulation, CD8+ T cells, cytotoxic T lymphocytes, P815 tumor cells, CTLA-4, IL-12, interleukin-6, T cell proliferation, accessory cells
MeSH terms
Abatacept, Animals, Antibodies, Monoclonal, Antigens, Differentiation, Base Sequence, Cells, Cultured, Cytotoxicity, Immunologic, Female, Interleukin-2, Leukemia L1210, Lymphocyte Culture Test, Mixed, Lymphocyte Activation, Membrane Glycoproteins, Mice, Inbred C57BL, Mice, Inbred DBA, Molecular Sequence Data, Recombinant Proteins, Mast-Cell Sarcoma, Specific Pathogen-Free Organisms, Spleen, T-Lymphocytes, Cytotoxic, Transfection, Tumor Cells, Cultured, Signal Transduction, CD4-Positive T-Lymphocytes, Antigens, CD, Interleukin-6, Muromonab-CD3, B7-1 Antigen, Interleukin-12, Immunoconjugates, Mice, B7-2 Antigen, CTLA-4 Antigen

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