The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Generation of Tumor Immunity by Bone Marrow-Derived Dendritic Cells Correlates with Dendritic Cell Maturation Stage
Abstract
Bone marrow-derived dendritic cells (BmDC) are potent APC and can promote antitumor immunity in mice when pulsed with tumor Ag. This study aimed to define the culture conditions and maturation stages of BmDC that enable them to optimally function as APC in vivo. BmDC cultured under various conditions (granulocyte-macrophage CSF (GM-CSF) or GM-CSF plus IL-4 alone or in combination with Flt3 ligand, TNF-α, LPS, or CD40 ligand (CD40L)) were analyzed morphologically, phenotypically, and functionally and were tested for their ability to promote prophylactic and/or therapeutic antitumor immunity. Each of the culture conditions generated typical BmDC. Whereas cells cultured in GM-CSF alone were functionally immature, cells incubated with CD40L or LPS were mature BmDC, as evident by morphology, capacity to internalize Ag, migration into regional lymph nodes, IL-12 secretion, and alloantigen or peptide Ag presentation in vitro. The remaining cultures exhibited intermediate dendritic cell maturation. The in vivo Ag-presenting capacity of BmDC was compared with respect to induction of both protective tumor immunity and immunotherapy of established tumors, using the poorly immunogenic squamous cell carcinoma, KLN205. In correspondence to their maturation stage, BmDC cultured in the presence of CD40L exhibited the most potent immunostimulatory effects. In general, although not entirely, the capacity of BmDC to induce an antitumor immune response in vivo correlated to their degree of maturation. The present data support the clinical use of mature, rather than immature, tumor Ag-pulsed dendritic cells as cancer vaccines and identifies CD40L as a potent stimulus to enhance their in vivo Ag-presenting capacity.
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Citations
Cites
Dendritic cells and the control of immunity
Banchereau, J., & Steinman, R. M. (1998) · Nature · doi:10.1038/32588 · Cited by 14,336
Sallusto, F., & Lanzavecchia, A. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.179.4.1109 · Cited by 5,129
The Dendritic Cell System and its Role in Immunogenicity
Steinman, R. M. (1991) · Annual Review of Immunology · doi:10.1146/annurev.iy.09.040191.001415 · Cited by 4,658
Inaba, K., Inaba, M., Romani, N., Aya, H., Deguchi, M., Ikehara, S., Muramatsu, S., & Steinman, R. M. (1992) · The Journal of experimental medicine · doi:10.1084/jem.176.6.1693 · Cited by 3,738
T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity
Gillis, S., Ferm, M. M., Ou, W., & Smith, K. A. (1978) · The Journal of Immunology · doi:10.4049/jimmunol.120.6.2027 · Cited by 3,273
Cella, M., Scheidegger, D., Palmer-Lehmann, K., Lane, P., Lanzavecchia, A., & Alber, G. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.2.747 · Cited by 1,983
Kobayashi, M., Fitz, L., Ryan, M., Hewick, R. M., Clark, S. C., Chan, S., Loudon, R., Sherman, F., Perussia, B., & Trinchieri, G. (1989) · The Journal of experimental medicine · doi:10.1084/jem.170.3.827 · Cited by 1,953
GM-CSF and TNF-α cooperate in the generation of dendritic Langerhans cells
Caux, C., Dezutter-Dambuyant, C., Schmitt, D., & Banchereau, J. (1992) · Nature · doi:10.1038/360258a0 · Cited by 1,554
Origin, maturation and antigen presenting function of dendritic cells
Cella, M., Sallusto, F., & Lanzavecchia, A. (1997) · Current Opinion in Immunology · doi:10.1016/s0952-7915(97)80153-7 · Cited by 1,320
Jonuleit, H., Kühn, U., Müller, G., Steinbrink, K., Paragnik, L., Schmitt, E., Knop, J., & Enk, A. H. (1997) · European Journal of Immunology · doi:10.1002/eji.1830271209 · Cited by 1,163
Cited by
Immunosuppressive networks in the tumour environment and their therapeutic relevance
Zou, W. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1586 · Cited by 2,057
Antigen Presentation in Extracellular Matrix
Gunzer, M., Schäfer, A., Borgmann, S., Grabbe, S., Zänker, K. S., Bröcker, E.-B., Kämpgen, E., & Friedl, P. (2000) · Immunity · doi:10.1016/s1074-7613(00)00032-7 · Cited by 414
Self-citationEpidermal RANKL controls regulatory T-cell numbers via activation of dendritic cells
Loser, K., Mehling, A., Loeser, S., Apelt, J., Kuhn, A., Grabbe, S., Schwarz, T., Penninger, J. M., & Beissert, S. (2006) · Nature Medicine · doi:10.1038/nm1518 · Cited by 402
Self-citationLiver regeneration and inflammation: from fundamental science to clinical applications
Campana, L., Esser, H., Huch, M., & Forbes, S. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00373-7 · Cited by 307
Averill, M. M., Barnhart, S., Becker, L., Li, X., Heinecke, J. W., LeBoeuf, R. C., Hamerman, J. A., Sorg, C., Kerkhoff, C., & Bornfeldt, K. E. (2011) · Circulation · doi:10.1161/circulationaha.110.985523 · Cited by 155
Metadata
- Authors
- Labeur, M. S., Roters, B., Pers, B., Mehling, A., Luger, T. A., Schwarz, T., & Grabbe, S.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-01-01
- Volume
- 162
- Issue
- 1
- Pages
- 168-175
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1790202563
- Cited by
- 422
- References
- 38
- FWCI
- 13.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- Labeur, M. S., Roters, B., Pers, B., Mehling, A., Luger, T. A., Schwarz, T., & Grabbe, S. (1999). Generation of Tumor Immunity by Bone Marrow-Derived Dendritic Cells Correlates with Dendritic Cell Maturation Stage. The Journal of Immunology. https://doi.org/10.4049/jimmunol.162.1.168
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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