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Cited by 253

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Human Endothelial-Cell Specific Molecule-1 Binds Directly to the Integrin CD11a/CD18 (LFA-1) and Blocks Binding to Intercellular Adhesion Molecule-1

Béchard, David, Scherpereel, Arnaud, Hammad, Hamida, Gentina, Thibaut, Tsicopoulos, Anne, Aumercier, Marc, Pestel, Jöel, Dessaint, Jean-Paul, Tonnel, André-Bernard, Lassalle, Philippe·2001·The Journal of Immunology
DOI 10.4049/jimmunol.167.6.3099
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Abstract

ICAMs are ligands for LFA-1, a major integrin of mononuclear cells involved in the immune and inflammatory processes. We previously showed that endothelial cell specific molecule-1 (ESM-1) is a proteoglycan secreted by endothelial cells under the control of inflammatory cytokines. Here, we demonstrate that ESM-1 binds directly to LFA-1 onto the cell surface of human blood lymphocytes, monocytes, and Jurkat cells. The binding of ESM-1 was equally dependent on Ca2+, Mg2+, or Mn2+ divalent ions, which are specific, saturable, and sensitive to temperature. An anti-CD11a mAb or PMA induced a transient increase in binding, peaking 5 min after activation. Direct binding of ESM-1 to LFA-1 integrin was demonstrated by specific coimmunoprecipitation by CD11a and CD18 mAbs. A cell-free system using a Biacore biosensor confirmed that ESM-1 and LFA-1 dynamically interacted in real time with high affinity (Kd = 18.7 nM). ESM-1 consistently inhibited the specific binding of soluble ICAM-1 to Jurkat cells in a dose-dependent manner. These results suggest that ESM-1 and ICAM-1 interact with LFA-1 on binding sites very close to but distinct from the I domain of CD11a. Through this mechanism, ESM-1 could be implicated in the regulation of the LFA-1/ICAM-1 pathway and may therefore influence both the recruitment of circulating lymphocytes to inflammatory sites and LFA-1-dependent leukocyte adhesion and activation.

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Metadata

Authors
Béchard, D., Scherpereel, A., Hammad, H., Gentina, T., Tsicopoulos, A., Aumercier, M., Pestel, J., Dessaint, J.-P., Tonnel, A.-B., & Lassalle, P.
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2001-09
Volume
167
Issue
6
Pages
3099-3106
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.167.6.3099
OpenAlex
W1500686316
Cited by
253
References
23
FWCI
1.64
Type
journal article
Language
en
Open access
closed

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