The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses
Open accessAbstract
Leukocyte responsiveness to LPS is dependent upon CD14 and receptors of the Toll-like receptor (TLR) family. Neutrophils respond to LPS, but conflicting data exist regarding LPS responses of eosinophils and basophils, and expression of TLRs at the protein level in these granulocyte lineages has not been fully described. We examined the expression of TLR2, TLR4, and CD14 and found that monocytes expressed relatively high levels of cell surface TLR2, TLR4, and CD14, while neutrophils also expressed all three molecules, but at low levels. In contrast, basophils expressed TLR2 and TLR4 but not CD14, while eosinophils expressed none of these proteins. Tested in a range of functional assays including L-selectin shedding, CD11b up-regulation, IL-8 mRNA generation, and cell survival, neutrophils responded to LPS, but eosinophils and basophils did not. In contrast to previous data, we found, using monocyte depletion by negative magnetic selection, that neutrophil responses to LPS were heavily dependent upon the presence of a very low level of monocytes, and neutrophil survival induced by LPS at 22 h was monocyte dependent. We conclude that LPS has little role in the regulation of peripheral blood eosinophil and basophil function, and that, even in neutrophils, monocytes orchestrate many previously observed leukocyte LPS response patterns.
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Takeuchi, O., Hoshino, K., Kawai, T., Sanjo, H., Takada, H., Ogawa, T., Takeda, K., & Akira, S. (1999) · Immunity · doi:10.1016/s1074-7613(00)80119-3 · Cited by 3,362
MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4
Shimazu, R., Akashi, S., Ogata, H., Nagai, Y., Fukudome, K., Miyake, K., & Kimoto, M. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.11.1777 · Cited by 2,185
Ozinsky, A., Underhill, D. M., Fontenot, J. D., Hajjar, A. M., Smith, K. D., Wilson, C. B., Schroeder, L., & Aderem, A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.250476497 · Cited by 2,085
Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus
Kurt-Jones, E. A., Popova, L., Kwinn, L., Haynes, L. M., Jones, L. P., Tripp, R. A., Walsh, E. E., Freeman, M. W., Golenbock, D. T., Anderson, L. J., et al. (2000) · Nature Immunology · doi:10.1038/80833 · Cited by 1,683
Ohashi, K., Burkart, V., Flohé, S., & Kolb, H. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.2.558 · Cited by 1,606
Cell Activation and Apoptosis by Bacterial Lipoproteins Through Toll-like Receptor-2
Aliprantis, A. O., Yang, R.-B., Mark, M. R., Suggett, S., Devaux, B., Radolf, J. D., Klimpel, G. R., Godowski, P., & Zychlinsky, A. (1999) · Science · doi:10.1126/science.285.5428.736 · Cited by 1,474
Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction
Fitzgerald, K. A., Palsson-McDermott, E. M., Bowie, A. G., Jefferies, C. A., Mansell, A. S., Brady, G., Brint, E., Dunne, A., Gray, P., Harte, M. T., et al. (2001) · Nature · doi:10.1038/35092578 · Cited by 1,256
Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling
Yang, R.-B., Mark, M. R., Gray, A., Huang, A., Xie, M. H., Zhang, M., Goddard, A., Wood, W. I., Gurney, A. L., & Godowski, P. J. (1998) · Nature · doi:10.1038/26239 · Cited by 1,205
Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products
Colotta, F., Re, F., Polentarutti, N., Sozzani, S., & Mantovani, A. (1992) · Blood · doi:10.1182/blood.v80.8.2012.2012 · Cited by 1,158
Hirschfeld, M., Ma, Y., Weis, J. H., Vogel, S. N., & Weis, J. J. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.2.618 · Cited by 1,153
Cited by
TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling
Ciesielska, A., Matyjek, M., & Kwiatkowska, K. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03656-y · Cited by 1,420
Eosinophils: Biological Properties and Role in Health and Disease
HOGAN, S. P., ROSENBERG, H. F., MOQBEL, R., PHIPPS, S., FOSTER, P. S., LACY, P., KAY, A. B., & ROTHENBERG, M. E. (2008) · Clinical & Experimental Allergy · doi:10.1111/j.1365-2222.2008.02958.x · Cited by 834
Neutrophil apoptosis and the resolution of infection
Kennedy, A. D., & DeLeo, F. R. (2009) · Immunologic Research · doi:10.1007/s12026-008-8049-6 · Cited by 400
Neutrophil apoptosis pathways and their modifications in inflammation
Simon, H. (2003) · Immunological Reviews · doi:10.1034/j.1600-065x.2003.00038.x · Cited by 347
Yang, K., Puel, A., Zhang, S., Eidenschenk, C., Ku, C.-L., Casrouge, A., Picard, C., von Bernuth, H., Senechal, B., Plancoulaine, S., et al. (2005) · Immunity · doi:10.1016/j.immuni.2005.09.016 · Cited by 272
Metadata
- Authors
- Sabroe, I., Jones, E. C., Usher, L. R., Whyte, M. K. B., & Dower, S. K.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-05-01
- Volume
- 168
- Issue
- 9
- Pages
- 4701-4710
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1895807078
- Cited by
- 383
- References
- 57
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Sabroe, I., Jones, E. C., Usher, L. R., Whyte, M. K. B., & Dower, S. K. (2002). Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses. The Journal of Immunology. https://doi.org/10.4049/jimmunol.168.9.4701
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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