Increase in Phagocytosis after Geldanamycin Treatment or Heat Shock: Role of Heat Shock Proteins

Vega, Virginia L; De Maio, Antonio

doi:10.4049/jimmunol.175.8.5280

Cited by 41Heat shock proteins researchPhagocytosis and Immune RegulationImmune Response and Inflammation

Increase in Phagocytosis after Geldanamycin Treatment or Heat Shock: Role of Heat Shock Proteins

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Vega, Virginia L, De Maio, Antonio·2005·The Journal of Immunology

DOI 10.4049/jimmunol.175.8.5280

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Abstract

The response to injury is activated at the systemic and cellular levels. At the systemic level, phagocytosis plays a key role in controlling infections and clearing necrotic and apoptotic cells. The expression of heat shock proteins (Hsp), which is a well-conserved process, is a major component of cellular response to stress. This study investigated the relationship between Hsps and phagocytosis. An increase in the phagocytosis of opsonized bacteria particles and latex beads was observed upon incubation of murine macrophages with geldanamycin (GA), a specific inhibitor of the Hsp90 family of proteins. The effect of GA on phagocytosis was blocked by coincubation with inhibitors of transcription (actinomycin D) or translation (cycloheximide), suggesting that gene expression was required. Because expression of Hsps has been observed after GA treatment, the effect of heat shock on phagocytosis was investigated. Similar to GA treatment, heat shock resulted in an actinomycin D-sensitive elevation of phagocytosis, which suggests that Hsps are involved. The increase in phagocytosis after GA treatment was not due to increased binding of opsonized particles to their respective receptors on the macrophage surface or to elevated oxidative stress. However, it was correlated with a rapid polymerization of actin in proximity to the plasma membrane. These results suggest that Hsps play a role in the modulation of the phagocytic process, which is part of the stress response.

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HSPs have been shown to modulate inflammatory response and phagocytosis in macrophages, though their mechanism of action remains unknown.
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From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [53]

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“Hsps are expressed after GA treatment because Hsp90 is a negative regulator of HSF-1 (7, 25, 26).”
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Authors: Vega, V. L., & De Maio, A.
Affiliations: Johns Hopkins University (US)
Division of Pediatric Surgery, Johns Hopkins University School of Medicine , Baltimore, MD 21205
ORCID: Vega, Virginia L
Venue: The Journal of Immunology
Publisher: Oxford University Press (OUP)
Published: 2005-10
Volume: 175
Issue: 8
Pages: 5280-5287
ISSN: 0022-1767 (ISSN-L)
1550-6606
DOI: 10.4049/jimmunol.175.8.5280
OpenAlex: W1584354252
Cited by: 41
References: 54
Citations by year: 2025: 1 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 2
FWCI: 1.13
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations: The Journal of Immunology (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Institute of General Medical Sciences (GM50878, R01GM050878)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords: phagocytosis, heat shock proteins, geldanamycin, HSP90 inhibition, heat shock, macrophages, actin polymerization, latex beads, stress response, actinomycin D, cycloheximide, gene expression
MeSH terms: Actins, Animals, Cell Line, Gene Expression Regulation, Hot Temperature, Heat-Shock Proteins, Immunoglobulin G, Macrophages, Microspheres, Phagocytosis, Quinones, Benzoquinones, Oxidative Stress, Lactams, Macrocyclic, Mice

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Increase in Phagocytosis after Geldanamycin Treatment or Heat Shock: Role of Heat Shock Proteins

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