Proteomics Fingerprinting of Phagosome Maturation and Evidence for the Role of a Gα during Uptake
Open accessAbstract
Phagocytosis, whether of food particles in protozoa or bacteria and cell remnants in the metazoan immune system, is a conserved process. The particles are taken up into phagosomes, which then undergo complex remodeling of their components, called maturation. By using two-dimensional gel electrophoresis and mass spectrometry combined with genomic data, we identified 179 phagosomal proteins in the amoeba Dictyostelium, including components of signal transduction, membrane traffic, and the cytoskeleton. By carrying out this proteomics analysis over the course of maturation, we obtained time profiles for 1,388 spots and thus generated a dynamic record of phagosomal protein composition. Clustering of the time profiles revealed five clusters and 24 functional groups that were mapped onto a flow chart of maturation. Two heterotrimeric G protein subunits, Galpha4 and Gbeta, appeared at the earliest times. We showed that mutations in the genes encoding these two proteins produce a phagocytic uptake defect in Dictyostelium. This analysis of phagosome protein dynamics provides a reference point for future genetic and functional investigations.
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Manual annotation of the microarray data detected 25 protein products in common with the 179 proteins identified in the phagosomal proteomic profile.
“manual annotation of the microarray data detected 25 protein products in common with the 179 proteins identified in the phagosomal proteomic profile.”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [35]
The putative proteins encoded by four genes assigned to phagocytic vesicle were recently found in a proteomic study to be enriched in phagosomes.
“The encoded putative proteins were recently found in a proteomic study to be enriched in phagosomes [35], which justifies their GO assignment to phagocytic vesicle.”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [35]
Cell signalling during phagocytosis is virtually unknown in Dictyostelium, except for the involvement of heterotrimeric G protein and PLC.
“Cell signalling during phagocytosis is virtually unknown in Dictyostelium, except for the involvement of the heterotrimeric G protein and PLC in the process [1,5,35].”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [35]
Soldati and coworkers resolved the time-course of over 1000 spots in purified phagosomes and identified by mass spectrometry 179 proteins.
“They resolved the time-course of over 1000 spots in purified phagosomes and identified by mass spectrometry 179 proteins.”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [35]
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- Authors
- Gotthardt, D., Blancheteau, V., Bosserhoff, A., Ruppert, T., Delorenzi, M., & Soldati, T.
- Affiliations
- University Hospital Heidelberg (DE)
- Imperial College London (GB)
- Heidelberg University (DE)
- SIB Swiss Institute of Bioinformatics (CH)
- University of Geneva (CH)
- Corresponding author
- Soldati, Thierry
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2006-12
- Volume
- 5
- Issue
- 12
- Pages
- 2228-2243
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2171251887
- Cited by
- 98
- References
- 71
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 1 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 7 · 2012: 11
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular & Cellular Proteomics (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- IRIS (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- National Science Foundation
- Wellcome Trust
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Max-Planck-Gesellschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- phagosome maturation, phagocytosis, Dictyostelium, G protein alpha subunit, Gβ, heterotrimeric G proteins, temporal profiles, signal transduction, membrane traffic, cytoskeleton
- MeSH terms
- Animals, Cells, Cultured, Dictyostelium, Models, Biological, Phagocytosis, Phagosomes, Electrophoresis, Gel, Two-Dimensional, Cluster Analysis, Animals, Genetically Modified, Proteomics, GTP-Binding Protein alpha Subunits, GTP-Binding Protein beta Subunits
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