Cited by 98Cellular transport and secretionAdvanced Proteomics Techniques and ApplicationsAutophagy in Disease and Therapy

Proteomics Fingerprinting of Phagosome Maturation and Evidence for the Role of a Gα during Uptake

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Gotthardt, Daniel, Blancheteau, Vincent, Bosserhoff, Armin, Ruppert, Thomas, Delorenzi, Mauro, Soldati, Thierry·2006·Molecular & Cellular Proteomics

Abstract

Phagocytosis, whether of food particles in protozoa or bacteria and cell remnants in the metazoan immune system, is a conserved process. The particles are taken up into phagosomes, which then undergo complex remodeling of their components, called maturation. By using two-dimensional gel electrophoresis and mass spectrometry combined with genomic data, we identified 179 phagosomal proteins in the amoeba Dictyostelium, including components of signal transduction, membrane traffic, and the cytoskeleton. By carrying out this proteomics analysis over the course of maturation, we obtained time profiles for 1,388 spots and thus generated a dynamic record of phagosomal protein composition. Clustering of the time profiles revealed five clusters and 24 functional groups that were mapped onto a flow chart of maturation. Two heterotrimeric G protein subunits, Galpha4 and Gbeta, appeared at the earliest times. We showed that mutations in the genes encoding these two proteins produce a phagocytic uptake defect in Dictyostelium. This analysis of phagosome protein dynamics provides a reference point for future genetic and functional investigations.

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Crossref · 10.1074/mcp.m600113-mcp200

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Authors
Gotthardt, D., Blancheteau, V., Bosserhoff, A., Ruppert, T., Delorenzi, M., & Soldati, T.
Affiliations
  • University Hospital Heidelberg (DE)
  • Imperial College London (GB)
  • Heidelberg University (DE)
  • SIB Swiss Institute of Bioinformatics (CH)
  • University of Geneva (CH)
Corresponding author
Soldati, Thierry
Venue
Molecular & Cellular Proteomics
Publisher
Elsevier BV
Published
2006-12
Volume
5
Issue
12
Pages
2228-2243
ISSN
1535-9476 (ISSN-L)
OpenAlex
W2171251887
Cited by
98
References
71
Citations by year
2026: 1 · 2025: 4 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 1 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 7 · 2012: 11
FWCI
3.57
Type
journal article
Language
en
Open access
Locations
  • Molecular & Cellular Proteomics (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
  • IRIS (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
  • National Science Foundation
  • Wellcome Trust
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
  • Max-Planck-Gesellschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
phagosome maturation, phagocytosis, Dictyostelium, G protein alpha subunit, Gβ, heterotrimeric G proteins, temporal profiles, signal transduction, membrane traffic, cytoskeleton
MeSH terms
Animals, Cells, Cultured, Dictyostelium, Models, Biological, Phagocytosis, Phagosomes, Electrophoresis, Gel, Two-Dimensional, Cluster Analysis, Animals, Genetically Modified, Proteomics, GTP-Binding Protein alpha Subunits, GTP-Binding Protein beta Subunits

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