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Comparison of a conventional polymerase chain reaction with real-time polymerase chain reaction for the detection of neurotropic viruses in cerebrospinal fluid samples
Open accessAbstract
PURPOSE: To compare a conventional polymerase chain reaction (PCR) and real-time PCR for the detection of neurotropic DNA viruses. MATERIALS AND METHODS: A total of 147 cerebrospinal fluid (CSF) samples was collected from patients attending a tertiary care hospital in South India for a period from 2005 to 2008. All these samples were tested using a conventional multiplex/uniplex PCR and a real-time multiplex/uniplex PCR. This technique was used to detect a large number of herpes viruses responsible for central nervous system infections, including HSV-1, HSV-2, VZV, CMV and EBV and the polyoma virus JCV. RESULTS: Overall, in the entire set of samples, the real-time PCR yielded 88 (59.9%) positives and conventional PCR had six (4.1%) positives. CONCLUSION: Our results suggest that the real-time PCR assay was more sensitive compared with the conventional PCR. The advantage of real-time PCR is that it can be performed much faster than conventional PCR. Real-time PCR is less time-consuming, less labour-intensive and also reduces the chance of contamination as there is no post-amplification procedure. In the entire study population, the major viruses detected using real-time PCR were EBV (34%), HSV-2 (10.8%) and VZV (6.8%).
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Metadata
- Authors
- Ramamurthy, M., Alexander, M., Aaron, S., Kannangai, R., Ravi, V, Sridharan, G., & Abraham, A.
- Affiliations
- Christian Medical College, Vellore (IN)
- Venue
- Indian Journal of Medical Microbiology
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 29
- Issue
- 2
- Pages
- 102-109
- ISSN
- 0255-0857 (ISSN-L)
- OpenAlex
- W2024670282
- Cited by
- 28
- References
- 17
- Citations by year
- 2025: 4 · 2024: 4 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Indian Journal of Medical Microbiology (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- TSpace (University of Toronto) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Herpesvirus Infections and Treatments
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Keywords
- real-time PCR, conventional PCR, neurotropic viruses, cerebrospinal fluid, herpesvirus, central nervous system infection, herpes simplex virus, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, diagnostic sensitivity, virus detection
- MeSH terms
- Cerebrospinal Fluid, Herpesviridae, Humans, India, JC Virus, Sensitivity and Specificity, Virology, Virus Diseases, Prevalence, Polymerase Chain Reaction, Clinical Laboratory Techniques
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