Solar ultraviolet radiation induces biological alterations in human skin in vitro: Relevance of a well-balanced UVA/UVB protection
Open accessAbstract
Cutaneous damages such as sunburn, pigmentation, and photoaging are known to be induced by acute as well as repetitive sun exposure. Not only for basic research, but also for the design of the most efficient photoprotection, it is crucial to understand and identify the early biological events occurring after ultraviolet (UV) exposure. Reconstructed human skin models provide excellent and reliable in vitro tools to study the UV-induced alterations of the different skin cell types, keratinocytes, fibroblasts, and melanocytes in a dose- and time-dependent manner. Using different in vitro human skin models, the effects of UV light (UVB and UVA) were investigated. UVB-induced damages are essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damages are mostly located within the dermal compartment. Pigmentation can also be obtained after solar simulated radiation exposure of pigmented reconstructed skin model. Those models are also highly adequate to assess the potential of sunscreens to protect the skin from UV-associated damage, sunburn reaction, photoaging, and pigmentation. The results showed that an effective photoprotection is provided by broad-spectrum sunscreens with a potent absorption in both UVB and UVA ranges.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
UV-B acts on keratinocytes, potentially induces DNA damage and mutation, stimulates release of soluble cytokines, and causes skin cells to show aging, inflammation, apoptosis, and carcinogenesis.
“UV-B acts on keratinocytes in the skin epithelial layer, potentially induces DNA damage and mutation in keratinocytes, stimulates the release of soluble cytokines from keratinocytes, and causes skin cells to show symptoms such as aging, inflammation, apoptosis, and carcinogenesis [25,26].”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [25]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 30 · Cited by 54
Research works printed but not linked: 10
- Eller MS. Repair of DNA photodamage in human skin. In: Gilchrest BA, editor. Photodamage. Cambridge: Blackwell Science; 1995. p. 26-50.
- Asselineau D, Bernard BA, Bailly C, Darmon M. Epidermal morphogenesis and induction of 67 kDa keratin polypeptide by culture at the liquid-air interface. Exp Cell Res 1985;159:536-9.
- Bernerd F, Asselineau D. Successive alteration and recovery of epidermal differentiation and morphogenesis after specific UVB-damages in skin reconstructed in vitro. Dev Biol 1997;183:123-38.
- Vink AA, Berg RJW, de Grujil FR, Roza L, Baan RA. Induction, repair and accumulation of thymine dimers in the skin of UV- B-irradiated hairless mice. Carcinogenesis 1991;12:861-4.
- Young AR. The sunburn cell. Photodermatol 1987;4:127-34.
- Fagot D, Asselineau D, Bernerd F. Matrix metalloproteinase-1 production observed after solar-simulated radiation exposure is assumed by dermal fibroblasts but involves a paracrine activation through epidermal keratinocytes. Photochem Photobiol 2004;79:499-505.
- Lavker RM. Cutaneous aging: Chronologic versus photoaging. In: Gilchrest BA, editor. Photodamage. Cambridge: Blackwell Science; 1995. p. 123-35.
- Seite S, Moyal D, Richard S, de Rigal J, Leveque JL, Hourseau C, et al. Mexoryl ® SX: A Broad-spectrum UVA filter protects human skin from the effects of repeated suberythemal doses of UVA. J Photochem Photobiol B 1998;44:69-76.
- Lahmann C, Young AR, Wittern KP, Bergemann J. Induction of mRNA for matrix metalloproteinase 1 and tissue inhibitor of metalloproteinases 1 in human skin in vivo by solar simulated radiation. Photochem Photobiol 2001;73:657-63.
- Sanchez NP, Pathak MA, Sato S, Fitzpatrick TB, Sanchez L, Mihm M, et al. A clinical light microscopic, ultrastructural and immunofluorescence study. J Am Acad Dermatol 1981;4:698-710.
- Authors
- Bernerd, F., Marionnet, C., & Duval, C.
- Affiliations
- L'Oréal (France) (FR)
- Venue
- Indian Journal of Dermatology Venereology and Leprology
- Publisher
- Scientific Scholar
- Published
- 2012
- Volume
- 78
- Issue
- 7
- Pages
- 15
- ISSN
- 0378-6323 (ISSN-L)
- OpenAlex
- W2055704481
- Cited by
- 54
- References
- 30
- Citations by year
- 2025: 2 · 2024: 9 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 2 · 2019: 4 · 2018: 5 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 5 · 2013: 2
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Indian Journal of Dermatology Venereology and Leprology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
- Keywords
- solar ultraviolet radiation, UVA, UV-B radiation, reconstructed human skin model, photoprotection, sunburn, photoaging, skin pigmentation, sunburn cells, DNA damage, keratinocytes, melanocytes, fibroblasts, broad-spectrum sunscreen, simulated solar radiation
- MeSH terms
- Cells, Cultured, Dose-Response Relationship, Radiation, Fibroblasts, Humans, Melanocytes, Models, Biological, Skin, Sunlight, Sunscreening Agents, Time Factors, Ultraviolet Rays, Keratinocytes
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
