Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L
Open accessAbstract
BACKGROUND: In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far. OBJECTIVE: To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract. MATERIALS AND METHODS: The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds. RESULTS AND DISCUSSION: A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86μg/ml. CONCLUSION: This study demonstrates that M. indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.
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Compounds present in M. indica leaves may block enzymes responsible for de novo synthesis of cholesterol.
“Various compounds present in M. indica leaves may have a twofold impact: firstly, by blocking enzymes responsible for the de novo synthesis of cholesterol, and secondly, by competing with cholesterol absorption [84].”
From Mangifera indica L., By-Products, and Mangiferin on Cardio-Metabolic and Other Health Conditions: A Systematic Review (2023) · cites this paper as [84]
The Results and Discussion describe phytosterols competing with cholesterol absorption, but say this occurs “even though an increase in hepatic cholesterol synthesis.” The paper tests leaf compounds for cholesterol esterase inhibition, an absorption-related mechanism, rather than reporting that they block enzymes responsible for de novo cholesterol synthesis. (LLM, conf=0.76)
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Experimental — accuracy not yet measuredCompounds present in M. indica leaves may compete with cholesterol absorption.
“Various compounds present in M. indica leaves may have a twofold impact: firstly, by blocking enzymes responsible for the de novo synthesis of cholesterol, and secondly, by competing with cholesterol absorption [84].”
From Mangifera indica L., By-Products, and Mangiferin on Cardio-Metabolic and Other Health Conditions: A Systematic Review (2023) · cites this paper as [84]
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References 30 · Cited by 34
Research works printed but not linked: 23
- [1]Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive summary of the third report of the national cholesterol education program (NCEP) Expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III) JAMA 2001 285 2486 97 11368702
- [2]Ranjan N Management of hyperlipidemias: An update Indian J Dermatol Venereol Leprol 2009 75 452 62 19736423
- [3]Pisciotta L Bellocchio A Bertolini S Nutraceutical pill containing berberine versus ezetimibe on plasma lipid pattern in hypercholesterolemic subjects and its additive effect in patients with familial hypercholesterolemia on stable cholesterol-lowering treatment Lipids Health Dis 2012 11 123 22998978 PMC3477057
- [5]Ogier N Amiot MJ Georgé S Maillot M Mallmann C Maraninchi M LDL-cholesterol-lowering effect of a dietary supplement with plant extracts in subjects with moderate hypercholesterolemia Eur J Nutr 2013 52 547 57 22527287
- [6]Khare CP An Encyclopedia of Medicinal plants of India 2004 Springer New Delhi 151
- [8]Ichiki H Miura T Kubo M Ishihara E Komatsu Y Tanigawa K New antidiabetic compounds, mangiferin and its glucoside Biol Pharm Bull 1998 21 1389 90 9881663
- [9]Luczkiewicz P Kokotkiewicz A Dampc A Luczkiewicz M Mangiferin: A promising therapeutic agent for rheumatoid arthritis treatment Med Hypotheses 2014 83 570 4 25204703
- [10]Leiro J Arranz JA Yáñez M Ubeira FM Sanmartín ML Orallo F Expression profiles of genes involved in the mouse nuclear factor-kappa B signal transduction pathway are modulated by mangiferin Int Immunopharmacol 2004 4 763 78 15135318
- [12]Sato T Kawamoto A Tamura A Tatsumi Y Fujii T Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid) Chem Pharm Bull (Tokyo) 1992 40 721 4 1611684
- [13]Anila L Vijayalakshmi NR Flavonoids from Emblica officinalis and Mangifera indica-effectiveness for dyslipidemia J Ethnopharmacol 2002 79 81 7 11744299
- [16]Sangeetha KN Sujatha S Muthusamy VS Anand S Nithya N Velmurugan D 3beta-taraxerol of Mangifera indica, a PI3K dependent dual activator of glucose transport and glycogen synthesis in 3T3-L1 adipocytes Biochim Biophys Acta 2010 1800 359 66 20026188
- [17]Severi JA Lima ZP Kushima H Brito AR Santos LC Vilegas W Polyphenols with antiulcerogenic action from aqueous decoction of mango leaves (Mangifera indica L.) Molecules 2009 14 1098 110 19305363 PMC6254050
- [18]John S Thangapandian S Lazar P Son M Park C Lee KW New insights in the activation of human cholesterol esterase to design potent anti-cholesterol drugs Mol Divers 2014 18 119 31 24173651
- [19]Pietsch M Gutschow M Alternate substrate inhibition of cholesterol esterase by thieno [2,3-d] [1,3] oxazin-4-ones J Biol Chem 2002 277 24006 13 11959857
- [20]Krause BR Sliskovic DR Anderson M Homan R Lipid-lowering effects of WAY-121,898, an inhibitor of pancreatic cholesteryl ester hydrolase Lipids 1998 33 489 98 9625596
- [21]Bailey JM Gallo LL Gillespie J Inhibition of dietary cholesterol ester absorption by 3-BCP, a suicide inhibitor of cholesterol-esterase Biochem Soc Trans 1995 23 408S 8566296
- [22]Jeon SM Kim HS Lee TG Ryu SH Suh PG Byun SJ Lower absorption of cholesteryl oleate in rats supplemented with Areca catechu L. extract Ann Nutr Metab 2000 44 170 6 11111132
- [24]Muruganandan S Srinivasan K Gupta S Gupta PK Lal J Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats J Ethnopharmacol 2005 97 497 501 15740886
- [25]Miura T Iwamoto N Kato M Ichiki H Kubo M Komatsu Y The suppressive effect of mangiferin with exercise on blood lipids in type 2 diabetes Biol Pharm Bull 2001 24 1091 2 11558577
- [26]Muruganandan S Gupta S Kataria M Lal J Gupta PK Mangiferin protects the streptozotocin-induced oxidative damage to cardiac and renal tissues in rats Toxicology 2002 176 165 73 12093613
- [27]Bitzur R Cohen H Kamari Y Harats D Phytosterols: Another way to reduce LDL cholesterol levels Harefuah 2013 152 729 31 51 24482998
- [28]Castellanos-Jankiewicz A Del Bosque-Plata L Tejero ME Combined effect of plant sterols and dietary fiber for the treatment of hypercholesterolemia Plant Foods Hum Nutr 2014 69 93 100 24831917
- [29]Feng J Yang XW Wang RF Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of Aquilaria sinensis Phytochemistry 2011 72 242 7 21215978
- Authors
- Gururaja, G., Mundkinajeddu, D., Dethe, S., Sangli, G., Abhilash, K., & Agarwal, A.
- Affiliations
- Manipal Academy of Higher Education (IN)
- Natural Remedies (India) (IN)
- Corresponding author
- Gururaja, GM
- Venue
- Pharmacognosy Research
- Publisher
- Manuscript Technomedia LLP
- Published
- 2015
- Volume
- 7
- Issue
- 4
- Pages
- 355
- ISSN
- 0974-8490 (ISSN-L)
- PMC
- PMC4660515
- OpenAlex
- W2234385655
- Cited by
- 34
- References
- 30
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 4 · 2019: 3 · 2018: 4 · 2017: 4 · 2015: 1
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Pharmacognosy Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Medicinal Plants Board, Ministry of AYUSH, Government of India
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive natural compounds
- Health Sciences › Medicine › Surgery › Natural Products and Biological Research
- Keywords
- mango, mango leaf extract, cholesterol esterase inhibition, mangiferin, bioassay-guided fractionation, extract standardization
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