Exposure to 60% oxygen promotes migration and upregulates angiogenesis factor secretion in breast cancer cells
Open accessAbstract
Peri-operative factors, including anaesthetic drugs and techniques, may affect cancer cell biology and clinical recurrence. In breast cancer cells, we demonstrated that sevoflurane promotes migration and angiogenesis in high fractional oxygen but not in air. Follow-up analysis of the peri-operative oxygen fraction trial found an association between high inspired oxygen during cancer surgery and reduced tumor-free survival. Here we evaluated effects of acute, high oxygen exposure on breast cancer cell viability, migration and secretion of angiogenesis factors in vitro. MDA-MB-231and MCF-7 breast cancer cells were exposed to 21%, 30%, 60%, or 80% v/v O2 for 3 hours. Cell viability at 24 hours was determined by MTT and migration at 24 hours with the Oris™ Cell Migration Assay. Secretion of angiogenesis factors at 24 hours was measured via membrane-based immunoarray. Exposure to 30%, 60% or 80% oxygen did not affect cell viability. Migration of MDA-MB-231 and MCF-7 cells was increased by 60% oxygen (P = 0.012 and P = 0.007, respectively) while 30% oxygen increased migration in MCF-7 cells (P = 0.011). These effects were reversed by dimethyloxaloylglycine. In MDA-MB-231 cells high fractional oxygen increased secretion of angiogenesis factors monocyte chemotactic protein 1, regulated on activation normal T-cell expressed and vascular endothelial growth factor. In MCF-7 cells, interleukin-8, angiogenin and vascular endothelial growth factor secretion was significantly increased by high fractional oxygen. High oxygen exposure stimulates migration and secretion of angiogenesis factors in breast cancer cells in vitro.
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Respiratory hyperoxia in the early stages of tumorigenesis accelerates tumor development by promoting angiogenesis.
“respiratory hyperoxia in the early stages of tumorigenesis accelerates tumor development by promoting angiogenesis.”
From Hypoxia signaling in cancer: Implications for therapeutic interventions (2023) · cites this paper as [461]
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Cites
Mosmann, T. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90303-4 · Cited by 56,104 · see citing works
The Interleukin-8 Pathway in Cancer
Waugh, D. J. J., & Wilson, C. (2008) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-07-4843 · Cited by 2,103 · see citing works
Asparagine Hydroxylation of the HIF Transactivation Domain: A Hypoxic Switch
Lando, D., Peet, D. J., Whelan, D. A., Gorman, J. J., & Whitelaw, M. L. (2002) · Science · doi:10.1126/science.1068592 · Cited by 1,558 · see citing works
Kilgannon, J. H. (2010) · JAMA · doi:10.1001/jama.2010.707 · Cited by 875 · see citing works
(2000) · PubMed · Cited by 706 · see citing works
Belda, F. J. (2005) · JAMA · doi:10.1001/jama.294.16.2035 · Cited by 543 · see citing works
Austin, M., Wills, K. E., Blizzard, L., Walters, E. H., & Wood-Baker, R. (2010) · BMJ · doi:10.1136/bmj.c5462 · Cited by 508 · see citing works
Elvidge, G. P., Glenny, L., Appelhoff, R. J., Ratcliffe, P. J., Ragoussis, J., & Gleadle, J. M. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m511408200 · Cited by 464 · see citing works
Meyhoff, C. S., Wetterslev, J., Jørgensen, L. N., Henneberg, S. W., Høgdall, C. K., Lundvall, L., Svendsen, P.-E., Mollerup, H., Lunn, T. H., Simonsen, I., et al. (2009) · JAMA · doi:10.1001/jama.2009.1452 · Cited by 418 · see citing works
Can anaesthetic and analgesic techniques affect cancer recurrence or metastasis?
Heaney, Á., & Buggy, D. J. (2012) · British Journal of Anaesthesia · doi:10.1093/bja/aes421 · Cited by 320 · see citing works
Self-citationHyperbaric oxygen therapy and cancer—a review
Moen, I. N., & Stuhr, L. E. B. (2012) · Targeted Oncology · doi:10.1007/s11523-012-0233-x · Cited by 304 · see citing works
Association Between Hyperoxia and Mortality After Stroke
Rincón, F., Kang, J. Y., Maltenfort, M., Vibbert, M. D., Urtecho, J. S., Athar, M. K., Jallo, J., Pineda, C. C., Tzeng, D. L., McBride, W. C., et al. (2013) · Critical Care Medicine · doi:10.1097/ccm.0b013e3182a27732 · Cited by 238 · see citing works
Hopf, H. W., Gibson, J., Angeles, A. P., Constant, J. S., Feng, J. J., Rollins, M. D., Hussain, M., & Hunt, T. K. (2005) · Wound Repair and Regeneration · doi:10.1111/j.1524-475x.2005.00078.x · Cited by 225 · see citing works
Buckley, A., McQuaid, S., JOHNSON, P. A., & Buggy, D. J. (2014) · British Journal of Anaesthesia · doi:10.1093/bja/aeu200 · Cited by 208 · see citing works
Self-citationBenzonana, L. L., Perry, N. J. S., Watts, H. R., Yang, B., Perry, I. A., Coombes, R. C., Takata, M., & Ma, D. (2013) · Anesthesiology · doi:10.1097/aln.0b013e31829e47fd · Cited by 206 · see citing works
Autocrine functions of VEGF in breast tumor cells
Perrot‐Applanat, M., & Benedetto, M. D. (2012) · Cell Adhesion & Migration · doi:10.4161/cam.23332 · Cited by 169 · see citing works
The effects of high perioperative inspiratory oxygen fraction for adult surgical patients
Wetterslev, J., Meyhoff, C. S., Jørgensen, L. N., Gluud, C. N., Lindschou, J., & Rasmussen, L. S. (2015) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd008884.pub2 · Cited by 165 · see citing works
VEGF-targeted cancer therapy strategies: current progress, hurdles and future prospects
Duda, D. G., Batchelor, T. T., Willett, C. G., & Jain, R. K. (2007) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2007.04.001 · Cited by 140 · see citing works
Long-term Consequences of Anesthetic Management
Sessler, D. I. (2009) · Anesthesiology · doi:10.1097/aln.0b013e3181a913e1 · Cited by 124 · see citing works
Jaura, A., Flood, G., Gallagher, H. C., & Buggy, D. J. (2014) · British Journal of Anaesthesia · doi:10.1093/bja/aet581 · Cited by 121 · see citing works
Self-citationNitrous Oxide and Long-Term Morbidity and Mortality in the ENIGMA Trial
Leslie, K., Myles, P. S., Chan, M. T. V., Forbes, A. B., Paech, M. J., Peyton, P. J., SILBERT, B. S., & Williamson, E. (2010) · Anesthesia & Analgesia · doi:10.1213/ane.0b013e3181f7e2c4 · Cited by 117 · see citing works
Belda, F. J., Aguilera, L., n, J. G. A. D. L. A., Alberti, J., Vicente, R., ndiz, L. A. F., Rodriquez, R., Sessler, D. I., Aguilar, G., García‐Botello, S., et al. (2006) · Obstetrical & Gynecological Survey · doi:10.1097/01.ogx.0000201918.45088.1a · Cited by 105 · see citing works
Lissoni, P., Malugani, F., Bonfanti, A., Bucovec, R., Secondino, S., Brivio, F., Bravo, A. F., Ferrante, R., Vigoré, L., Rovelli, F., et al. (2001) · PubMed · Cited by 102 · see citing works
Bos, R., van Diest, P. J., van der Groep, P., Shvarts, A., Greijer, A. E., & van der Wall, E. (2004) · Breast Cancer Research · doi:10.1186/bcr813 · Cited by 77 · see citing works
The CCL5/CCR5 axis promotes metastasis in basal breast cancer
Velasco-Velázquez, M. A., & Pestell, R. G. (2013) · OncoImmunology · doi:10.4161/onci.23660 · Cited by 73 · see citing works
III. Oxygen therapy in anaesthesia: the yin and yang of O2
Martín, D., & Grocott, M. P. W. (2013) · British Journal of Anaesthesia · doi:10.1093/bja/aet291 · Cited by 69 · see citing works
Stall, A. C., Paryavi, E., Gupta, R. J., Zadnik, M., Hui, E., & O’Toole, R. V. (2013) · The Journal of Trauma: Injury, Infection, and Critical Care · doi:10.1097/ta.0b013e3182a1fe83 · Cited by 65 · see citing works
Oxygen and Surgical Site Infection
Qadan, M., Battista, C. T., Gardner, S. A., Anderson, G., Akça, O., & Polk, H. C. (2010) · Anesthesiology · doi:10.1097/aln.0b013e3181e19d1d · Cited by 64 · see citing works
Meyhoff, C. S., Jørgensen, L. N., Wetterslev, J., Siersma, V., & Rasmussen, L. S. (2014) · British Journal of Anaesthesia · doi:10.1093/bja/aeu110 · Cited by 64 · see citing works
Hyperoxia improves microvascular perfusion in a murine wound model
Sheikh, A. Y., Rollins, M. D., Hopf, H. W., & Hunt, T. K. (2005) · Wound Repair and Regeneration · doi:10.1111/j.1067-1927.2005.130313.x · Cited by 63 · see citing works
Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation.
Granowitz, E. V., Tonomura, N., Benson, R. M., Katz, D., Band, V., Makari‐Judson, G., & Osborne, B. A. (2005) · PubMed · Cited by 56 · see citing works
Hopf, H. W., & Holm, J. R. (2008) · Best practice & research. Clinical anaesthesiology · doi:10.1016/j.bpa.2008.06.001 · Cited by 55 · see citing works
Ash, S. A., & Buggy, D. J. (2013) · Best practice & research. Clinical anaesthesiology · doi:10.1016/j.bpa.2013.10.005 · Cited by 54 · see citing works
Self-citationAyrapetov, M. K., Xu, C., Sun, Y., Zhu, K., Parmar, K., D'Andrea, A. D., & Price, B. D. (2011) · PLoS ONE · doi:10.1371/journal.pone.0026064 · Cited by 41 · see citing works
Ershler, W. B., & Longo, D. L. (1997) · Cancer · doi:10.1002/(sici)1097-0142(19971001)80:7 · Cited by 41 · see citing works
Dutta, S., Bandyopadhyay, C., Bottero, V., Veettil, M. V., Wilson, L., Pins, M. R., Johnson, K. E., Warshall, C., & Chandran, B. (2014) · Molecular Oncology · doi:10.1016/j.molonc.2013.12.017 · Cited by 39 · see citing works
Gonzalez, R. M., Daly, D. S., Tan, R., Marks, J. R., & Zangar, R. C. (2011) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-10-1248 · Cited by 38 · see citing works
Ash, S. A., Valchev, G., Looney, M., Mhathuna, A. N., Crowley, P. D., Gallagher, H. C., & Buggy, D. J. (2014) · British Journal of Anaesthesia · doi:10.1093/bja/aeu191 · Cited by 37 · see citing works
Self-citationDoes Removal of the Primary Tumor in Metastatic Breast Cancer Improve Survival?
Rashid, O. M., & Takabe, K. (2013) · Journal of Women s Health · doi:10.1089/jwh.2013.4517 · Cited by 32 · see citing works
Cancer Neovascularization and Proinflammatory Microenvironments
Furuya, M., & Yonemitsu, Y. (2008) · Current Cancer Drug Targets · doi:10.2174/156800908784533481 · Cited by 30 · see citing works
Strachan, L. M., & Noble, D. W. (2001) · PubMed · Cited by 23 · see citing works
Directional Migration of MDA-MB-231 Cells Under Oxygen Concentration Gradients
Yahara, D., Yoshida, T., Enokida, Y., & Takahashi, E. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-38810-6_17 · Cited by 17 · see citing works
Oxygen: avoid too much of a good thing!
Rothen, H. U. (2010) · European Journal of Anaesthesiology · doi:10.1097/eja.0b013e3283396360 · Cited by 15 · see citing works
Serum level of angiogenin in breast cancer.
Sheen‐Chen, S., Eng, H., Chen, W. J., Chou, F., & Chen, H.-S. (2000) · PubMed · Cited by 15 · see citing works
Oxygen Use in the Perioperative Period. Should We Change Our Practice?
Grecu, L. (2012) · Analgesia & Resuscitation Current Research · doi:10.4172/2324-903x.1000e102 · Cited by 1 · see citing works
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Zhuang, Y., Liu, K., He, Q., Gu, X., Jiang, C., & Wu, J. (2023) · MedComm · doi:10.1002/mco2.203 · Cited by 119 · see citing works
Benefits and harms of increased inspiratory oxygen concentrations
Schwarte, L. A., Schober, P. R., & Loer, S. A. (2019) · Current Opinion in Anaesthesiology · doi:10.1097/aco.0000000000000791 · Cited by 23 · see citing works
Tiron, A., Ristescu, I., Postu, P. A., Tiron, C. E., Zugun‐Eloae, F., & Grigoraș, I. (2020) · Cancers · doi:10.3390/cancers12030688 · Cited by 13 · see citing works
Drzał, A., Delalande, A., Dziurman, G., Pichon, C., Swakoń, J., & Elas, M. (2023) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2023.02.022 · Cited by 9 · see citing works
Wang, H., Wang, Z., Wu, Q., Yang, Y., Liu, S., Bian, J., & Bo, L. (2024) · Medical Gas Research · doi:10.4103/mgr.medgasres-d-24-00010 · Cited by 8 · see citing works
Ristescu, I., Tiron, C. E., Tiron, A., & Grigoraș, I. (2021) · Biomedicines · doi:10.3390/biomedicines9091213 · Cited by 7 · see citing works
The Effect of Anaesthetic and Analgesic Technique on Oncological Outcomes
Sherwin, A., & Buggy, D. J. (2018) · Current anesthesiology reports · doi:10.1007/s40140-018-0299-9 · Cited by 3 · see citing works
Self-citation
- Authors
- Crowley, P., Stuttgen, V., O'Carroll, E., Ash, S., Buggy, D., & Gallagher, H.
- Affiliations
- University College Dublin (IE)
- Corresponding author
- Crowley, PeterD
- Venue
- Medical Gas Research
- Published
- 2017
- Volume
- 7
- Issue
- 4
- Pages
- 226
- ISSN
- 2045-9912 (ISSN-L)
- PMC
- PMC5806442
- OpenAlex
- W2784141775
- Cited by
- 7
- References
- 45
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Medical Gas Research (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Cancer, Stress, Anesthesia, and Immune Response
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Surgery › Biomedical and Chemical Research
- Keywords
- hyperoxia, breast cancer, tumor cell migration, angiogenic factors, sevoflurane, MDA-MB-231 cells, MCF-7 cells, MCP-1, interleukin-8, angiogenin, vascular endothelial growth factor, tumor-free survival
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