Establishment of C6 brain glioma models through stereotactic technique for laser interstitial thermotherapy research
Open accessAbstract
OBJECTIVE: To establish C6 brain glioma models using stereotactic technique, and to study effects of laser interstitial thermotherapy (LITT) in rat models of glioma. METHODS: C6 glioma cells were cultured in dulbecco's minimum essential medium (DMEM) cell culture medium. The in vitro C6 cell cultures were stereotaxically implanted into the right caudate nucleus of rat brain. Presence of tumor was confirmed with Factor VIII R, hematoxylin-eosin stain, staining of glial fibrillary acid protein, and S-100 immunohistochemistry. After magnetic resonance (MR) scanning and correction of tumor location, the models were divided into groups according to the treating time and laser power (2-10 W). Semiconductor laser optical fibers were inserted in tumors for LITT. Cortex's temperature conducted from the center target was measured using infrared thermograph, and deep-tissue temperature around the target was measured using a thermocouple. RESULTS: Rat C6 gliomas were inoculated with optimized stereotactic technique. These gliomas resembled human glioma in terms of histopathological features. Such models are more reliable and reproducible, with 100% yield of intracranial tumor and no extracranial growth extension. The difference between cortex temperature conducted from center target and deep-tissue temperature around target was not statistically significant. CONCLUSION: The rat C6 brain glioma model established in the study was a perfect model to study LITT of glioma. Infrared thermograph technique measured temperature conveniently and effectively. The technique is noninvasive, and the obtained data could be further processed using software used in LITT research. To measure deep-tissue temperature, combining thermocouple with infrared thermograph technique would present better results.
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C6 cell line has a close gene expression pattern to human brain tumors and is widely used in neuro-oncological studies.
“To confirm our results, we also employed C6 cell line for generating xenograft rat models, because of a close gene expression pattern to human brain tumors and they are also widely used in neuro-oncological studies (58–60).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [60]
The Discussion (page not provided) describes stereotactically implanted C6 glioma models as among the most widely used, supporting the claim about their use in neuro-oncological research. The Results report resemblance to human glioma in histopathological features, not a close gene-expression pattern; our copy does not establish that genetic claim. (LLM, conf=0.88)
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La qüestió catalana: ; (articles, 1989-1993)
Rahola, P. (1993) · Dialnet (Universidad de la Rioja)
- Authors
- Chen, M., Shi, J., Zhang, Y., Fu, W.-M., & Qiu, Z.
- Affiliations
- Taizhou First People's Hospital (CN)
- Second Affiliated Hospital of Zhejiang University (CN)
- Corresponding author
- Chen, Minjiang
- Venue
- Surgical Neurology International
- Publisher
- Scientific Scholar
- Published
- 2015
- Volume
- 6
- Issue
- 1
- Pages
- 51
- ISSN
- 2152-7806 (ISSN-L)
- PMC
- PMC4395983
- OpenAlex
- W2335220694
- Cited by
- 11
- References
- 21
- Citations by year
- 2024: 1 · 2023: 4 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1
- FWCI
- 0.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by
- Locations
- Surgical Neurology International (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- C6 glioma, stereotactic implantation, laser interstitial thermal therapy, rat glioma model, caudate nucleus, infrared thermography, thermocouples, semiconductor lasers, glioma histopathology
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