A validated RP-HPLC method for quantitation of trigonelline from herbal formulations containing Trigonella foenum-graecum (L.) seeds
Open accessAbstract
BACKGROUND: Trigonella foenum-graecum (L.) (Fabaceae, Fenugreek) is an important ingredient of Ayurvedic and other marketed herbal formulations. Fenugreek seeds are employed in many traditional systems as an antibacterial and antidiabetic agent, gastric stimulant and galactogogue. Trigonelline, a major phytoconstituent found in fenugreek seeds, shows estrogenic, anti-diabetic and anti-invasive activity. Therefore, it is a suitable bioactive marker to establish the quality of crude drug and its formulations. OBJECTIVE: To develop an efficient and effective RP-HPLC method for estimation of trigonelline from Trigonella foenum-graecum seeds and its marketed herbal formulations. MATERIALS AND METHODS: Separation and detection of trigonelline was carried out on a Cosmosil CN-MS column eluted with methanol:distilled water [95:5, v/v; pH 3.5 using hydrochloric acid]. Detection was carried out at 267 nm using a Photo Diode Array detector. Fenugreek seeds and two marketed herbal formulations were subjected for HPLC analysis of Trigonelline. RESULTS: The RP-HPLC method was validated as per ICH guidelines and the content of trigonelline in marketed polyherbal formulations such as Dibet powder and Amyron syrup was determined. The LOD and LOQ were found to be 5.00 ng/mL and 50.00 ng/mL, respectively. Detector response was linear from 100.00 to 8000.00 ng/mL. The method was found to be simple, sensitive, accurate, reproducible and rugged. CONCLUSION: This work can be recommended for quality assurance and marker-based standardization of formulations containing fenugreek seeds.
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Trigonelline is an important chemotaxonomic marker of Trigonella species.
“Trigonelline is an important chemotaxonomic marker of Trigonella species (Mohammadi et al., 2020;Shailajan et al., 2011;Zameer et al., 2018) and was found to be the major compound in the sample analysed herein.”
From Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease (2022) · cites this paper as Shailajan et al., 2011
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Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease
Fernandes, F., Barroso, M. F., De Simone, A., Emriková, E., Dias-Teixeira, M., Pereira, J. P., Chlebek, J., Fernandes, V. C., Rodrigues, F., Andrisano, V., et al. (2022) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2022.115107 · Cited by 45 · see citing works
- Authors
- Shailajan, S., Sayed, N., Menon, S., Singh, A., & Mhatre, M.
- Corresponding author
- Shailajan, Sunita
- Venue
- Pharmaceutical Methods
- Publisher
- Manuscript Technomedia LLP
- Published
- 2011-07
- Volume
- 2
- Issue
- 3
- Pages
- 157-160
- ISSN
- 2229-4708 (ISSN-L)
- PMC
- PMC3658058
- OpenAlex
- W2094771345
- Cited by
- 33
- References
- 13
- Citations by year
- 2026: 3 · 2025: 2 · 2023: 2 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 5 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 1 · 2013: 1
- FWCI
- 0.63
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- Pharmaceutical Methods (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Keywords
- trigonelline, fenugreek, fenugreek seeds, herbal formula, RP-HPLC method validation, quality control, bioactive markers, Ayurvedic formulation
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