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Biogenic Ag–Cu Nanoparticles Synthesized with Extract of Eryngium Billardieri L. and Evaluation of their Anticancer Potential on PC-3 and LNCaP Cancer Cell Lines

Ebrahim, Zahra, Feyzabadi, Ghazaleh, Asghari Moghaddam, Nastaran, Mohammadgholi, Azadeh·2025·Advanced Biomedical Research
DOI 10.4103/abr.abr_255_24
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Abstract

Background: The anticancer action of biogenic Ag-Cu nanoparticles (NPs) using Eryngium billardieri against PC-3 and LNCaP cell lines in prostate malignancy was investigated. Materials and Methods: XRD, FTIR, EDAX, FESEM, and TEM were used to illustrate the Ag–Cu NPs. The PC-3 and LNCaP cell lines were used to test the anticancer ability of Ag–Cu NPs. The cytotoxicity was assessed by MMT and flow cytometry. Apoptotic and metastatic-promoting gene expressions were evaluated by real-time PCR. ROS generation was measured. Results: NPs are spherical and their average size was 12 ± 9.7 nanometers. The EDAX result indicated the presence of Ag, Cu, and C. The XRD results revealed the NPs’ crystalline structure. The results demonstrated a remarkable antiproliferative effect of the nanoparticles, with IC50 values of 15.43 μg/mL for PC-3 and 7.64 μg/mL for LNCaP. The Ag–Cu NPs exhibited a tendency to trigger apoptosis. This was confirmed by analyzing apoptosis-related gene expression. The apoptotic influence of Ag–Cu NPs was suggested to be critical when compared to the control and extract-treated groups by the up-regulation of Bcl2 -related X ( Bax ), caspase3 , and caspase9 , and the down-regulation of Bcl2 . Furthermore, Annexin V-FITC/propidium iodide presented a 23.15% and 22.3% apoptotic ratio of PC-3 and LNCaP cells, respectively. Vascular endothelial growth factor ( VEGF ), and metalloproteinases (MMPs) down-regulation showed that Ag–Cu NPs were hostile to metastasis. Also, ROS generation was estimated at 1261 and 1366 RFU in LNCaP and PC-3 cells treated with green-synthesized Ag–Cu NPs, respectively. Conclusions: The study suggested E. billardieri synthesized Ag–Cu as a potential candidate in prostate cancer therapeutic management.

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Metadata

Authors
Ebrahim, Z., Feyzabadi, G., Asghari Moghaddam, N., & Mohammadgholi, A.
Venue
Advanced Biomedical Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2025-05
Volume
14
Issue
1
ISSN
2277-9175
DOI
10.4103/abr.abr_255_24
Cited by
0
Language
en
Licence
creativecommons.org/licenses/by-nc-sa/4.0 (unspecified)

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CoChat. (2026). Citation check: Biogenic Ag–Cu Nanoparticles Synthesized with Extract of Eryngium Billardieri L. and Evaluation of their Anticancer Potential on PC-3 and LNCaP Cancer Cell Lines. https://app.cochat.ai/papers/10.4103/abr.abr_255_24
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Ebrahim, Z., Feyzabadi, G., Asghari Moghaddam, N., & Mohammadgholi, A. (2025). Biogenic Ag–Cu Nanoparticles Synthesized with Extract of Eryngium Billardieri L. and Evaluation of their Anticancer Potential on PC-3 and LNCaP Cancer Cell Lines. Advanced Biomedical Research. https://doi.org/10.4103/abr.abr_255_24

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