A wide-ranging cellular response to UV damage of DNA
Open accessAbstract
The kinases ATM and ATR are central to proper function of the DNA damage response. These kinases phosphorylate proteins to coordinate cell cycle progression and DNA damage repair/bypass. We have recently reported a large-scale identification of ATM/ATR substrates phosphorylated in response to UV damage of DNA. Overall 231 sites of phosphorylation were induced by UV damage of DNA or dependent on proper function of ATR. The study expanded the number of phosphorylation sites from protein classes known to be involved in the DNA damage response. Further, many sites were identified from protein types not thought to have a role in damage signaling. This observation suggests that the DNA damage response affects a much wider range of cellular processes than was previously appreciated. This study has also extended the successful use of the PhosphoScan proteomic method from phospho-tyrosine to serine/threonine motifs, providing a general blueprint to use the method to study signaling pathways underlying a wide range of diseases.
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Studies found 216 SQ/TQ sites phosphorylated in UV-damaged cells.
“What they found was the potential 216 SQ/TQ sites that were phosphorylated in UV-damaged cells.”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [70]
A total of 231 SQ/TQ sites are induced by UV damage dependent on ATR or both.
“This gave a total of 231 SQ/TQ sites induced by UV damage dependent on ATR or both.”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [70]
The activated signaling cascade leads to cellular responses such as DNA repair, chromatin modelling, transcription, autophagy, senescence, and apoptosis.
“The activated signaling cascade finally leads to various cellular responses such as DNA repair, chromatin modelling, transcription, autophagy, senescence, and apoptosis.”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [70]
ATM and ATR phosphorylate serine or threonine residues in the context of glutamine at the +1 position (the SQ/TQ motif).
“ATM and ATR phosphorylate serine or threonine residues in the context of glutamine at the +1 position (the SQ/TQ motif).”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [70]
The study confirmed 39 sites more highly phosphorylated in UV-damaged ATR-wild type cells compared to UV-damaged ATR-deficient cells.
“Furthermore, the study confirmed 39 sites more highly phosphorylated in UV-damaged ATR-wild type cells compared UV-damaged ATR-deficient cells.”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [70]
The damage response to UV radiation involved multiple versatile proteins including those involved in cell cycle progression, chromatin binding, transcriptional regulation, DNA repair, and other functions not typical for the damage response.
“The damage response to UV radiation involved multiple versatile proteins including those involved in cell cycle progression, chromatin binding, transcriptional regulation, DNA repair, and many other functions considered not typical for the damage response [70].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [70]
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- Authors
- Stokes, M., & Comb, M.
- Affiliations
- Cell Signaling Technology (United States) (US)
- ORCID
- Stokes, Matthew
- Venue
- Cell Cycle
- Publisher
- Informa UK Limited
- Published
- 2008-07-15
- Volume
- 7
- Issue
- 14
- Pages
- 2097-2099
- ISSN
- 1538-4101 (ISSN-L)
- 1551-4005
- OpenAlex
- W2062686677
- Cited by
- 21
- References
- 22
- Citations by year
- 2025: 2 · 2021: 1 · 2020: 1 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell Cycle (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- DNA damage response, UV-induced DNA damage, ATM kinase, ATR kinase, protein phosphorylation, phosphorylation sites, kinase substrates, serine/threonine phosphorylation, cell cycle regulation, DNA repair, DNA damage signaling
- MeSH terms
- DNA Damage, Humans, Phosphorylation, Phosphoserine, Phosphothreonine, Proteins, Ultraviolet Rays
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