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Cited by 10DNA Repair MechanismsCell death mechanisms and regulationGenetics, Aging, and Longevity in Model Organisms

Unrepaired oxidative DNA damage induces an ATR/ATM apoptotic-like response in quiescent fission yeast

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Arcangioli, Benoit, Ben Hassine, Samia·2009·Cell Cycle
DOI 10.4161/cc.8.15.9147
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Abstract

Programmed cell death is a term which refers to a genetic decision of self-killing or suicide of a cell. Programmed cell death is not restricted to multicellular organisms and was described in a wide range of unicellular eukaryotes, indicating phylogenetically conserved functions, that participate in an adaptive response to cellular stress. Here we review and discuss our observations recently published in the EMBO Journal,(1) that non-dividing fission yeast, Schizosaccharomyces pombe, exhibits a DNA damage response leading to cell death. We found that Tdp1 protects quiescent S. pombe cells against oxidative DNA damage. Tdp1 is a well-conserved tyrosyl-DNA phosphodiesterase required for single-strand break DNA repair, the mutation of Tdp1 is responsible for the recessively inherited syndrome spinocerebellar ataxia with axonal neuropathy (SCAN1) in humans. We found that tdp1 mutant yeast cells grow, as well as the wild-type cells, during the vegetative state, but progressively die in the quiescent state. We showed that, in the absence of Tdp1, the accumulation of unrepaired oxidative DNA damage triggers a genetic response, leading to checkpoint-dependent (ATM/ATR) nuclear DNA degradation, reminiscent of apoptosis. Our results indicate that the reactive oxygen species (ROS) produced during mitochondrial respiration are the main DNA damaging agents in the physiological quiescent state.

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Authors
Arcangioli, B., & Ben Hassine, S.
Venue
Cell Cycle
Publisher
Informa UK Limited
Published
2009-08
Volume
8
Issue
15
Pages
2326-2331
ISSN
  • 1538-4101 (ISSN-L)
  • 1551-4005
DOI
10.4161/cc.8.15.9147
OpenAlex
W2094943390
Cited by
10
References
0
Citations by year
2025: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2012: 2
FWCI
0.40
Type
journal article
Language
en
Open access
Locations
  • Cell Cycle (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Agence Nationale de la Recherche (ANR-06-BLAN-0271)
  • China Scholarship Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Keywords
oxidative DNA damage, programmed cell death, Schizosaccharomyces pombe, TDP1, tyrosyl-DNA phosphodiesterase, ATM, ATR, DNA damage response, quiescent cells, reactive oxygen species, mitochondrial respiration, nuclear DNA degradation
MeSH terms
DNA Damage, DNA Repair, DNA-Binding Proteins, Oxidation-Reduction, Phosphoric Diester Hydrolases, Protein Kinases, Schizosaccharomyces, Cell Death, Apoptosis, Protein Serine-Threonine Kinases, Reactive Oxygen Species, Cell Cycle Proteins, Tumor Suppressor Proteins, Schizosaccharomyces pombe Proteins, DNA Breaks, Single-Stranded, Ataxia Telangiectasia Mutated Proteins, Checkpoint Kinase 2

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