Evaluation of Various Extraction Techniques for Efficient Lipid Recovery from Thermo-Resistant Microalgae, Hindakia, Scenedesmus and Micractinium Species —Comparison of Lipid Extraction Methods from Microalgae
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In recent years, photosynthetic microalgae regained attention for biodiesel production. For efficient utilization of microalgae, a number of criteria including a strain with high biomass and lipid productivities and employment of effective and reliable methods for oil extraction from the obtained biomass should be met. Recently, we have isolated and identified three thermo-resistant green microalgae strains, namely; Scenedesmus sp. ME02, Hindakia tetrachotoma ME03 and Mic-ractinium sp. ME05. In this study, we compared percent lipid content of thermos-tolerant mic-roalgal strains using the following solvent extraction methods: Soxhlet, Bligh and Dyer and Folch methods with or without assisted cell disruption techniques including lyophilization, homogenization, ultrasonication, bead and microwave-assisted. The highest increase in lipid yield was obtained with a combination of lyophilization and ultrasonication techniques together with Soxhlet method: 27% of total dry weight for Micractinium sp. ME05. We conclude that lyophilization and ultrasonication are effective assistance methods for lipid extraction from thermo-resistant microalgae.
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The group of leaders in growth rate includes Micractinium sp. ME05 (5.2 day−1), Hindakia tetrachotoma ME03 (4.6 day−1), Scenedesmus sp. ME02 (3.5 day−1), Ettlia oleoabundans (1.69 day−1), Phaeodactylum tricornutum CCMP 2561 (1.63 day−1), Cryptomonas baltica NIVA-5/91 (1.4 day−1), Chlorella vulgaris (1.34 day−1), Tetraselmis suecica (1.32 day−1), Isochrysis sp. (1.32 day−1), Arthrospira platensis (
“The group of leaders is formed by: Micractinium sp. ME05 (5.2 day− 1), Hindakia tetrachotoma ME03 (4.6 day− 1), Scenedesmus sp. ME02 (3.5 day− 1) [92], Ettlia oleoabundans (1.69 day− 1) [93], Phaeodactylum tricornutum CCMP 2561 (1.63 day− 1), Cryptomonas baltica NIVA-5/91(1.4 day− 1) [94], Chlorella vulgaris (1.34 day− 1), Tetraselmis suecica (1.32 day− 1), Isochrysis sp. (1.32 day− 1) [93], Arth”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [92]
The citation follows the three named strains, and §2.1 identifies all three. However, §3.3 says their growth characteristics were described in an earlier study; this paper reports lipid productivities, not the claimed growth rates of 5.2, 4.6 and 3.5 day−1. No page numbers are available in our copy. (LLM, conf=0.84)
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References 33 · Cited by 30
Research works printed but not linked: 3
- Soxhlet, F. (1879) Die gewichtsanalytische Bestimmung des Milchfettes. Dinglers Polytechnisches Journal, 232, 461- 465.
- Ausborn, M., Nuhn, P. and Schreier, H. (1992) Stabilization of Liposomes by Freeze Thaw and Lyophilization Tech- niques: Problems and Opportunities. European Journal of Pharmaceutics and Biopharmaceutics, 38, 133-139.
- Nielsen, S.S. (2003) Food Analysis. 3rd Edition, Kluwer Academic/Plenum, New York.
- Authors
- Onay, M., Sonmez, C., Oktem, H. A., & Yucel, M.
- Affiliations
- Van Yüzüncü Yıl Üniversitesi (TR)
- ORCID
- Onay, Melih
- Venue
- American Journal of Analytical Chemistry
- Publisher
- Scientific Research Publishing, Inc.
- Published
- 2016
- Volume
- 07
- Issue
- 02
- Pages
- 141-150
- ISSN
- 2156-8251 (ISSN-L)
- 2156-8278
- OpenAlex
- W2266306059
- Cited by
- 30
- References
- 33
- Citations by year
- 2025: 7 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 6 · 2017: 1 · 2016: 1
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- American Journal of Analytical Chemistry (publishedVersion, cc-by)
- Funders
- Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (BİDEB 2211)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Engineering › Biomedical Engineering › Biodiesel Production and Applications
- Keywords
- microalgal lipid extraction, Scenedesmus, Micractinium, biodiesel feedstock, Soxhlet extraction, Bligh and Dyer method, Folch method, cell disruption, freeze-drying, sonication, microwave-assisted extraction, bead beating, lipid yield
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