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Development of novel polymorphic microsatellite markers for the silver fox (Vulpes vulpes)
Open accessAbstract
The silver fox (Vulpes vulpes), a coat color variant of the red fox, is one of the most important fur-bearing animals. To date, development of microsatellite loci for the silver fox has been limited and mainly based on cross-amplification by using canine SSR primers. In this study, 28 polymorphic microsatellite markers were isolated and identified for silver fox through the construction and screening of an (AC)n-enriched library. The number of alleles per locus ranged from 2 to 8 based on 48 individuals tested. The expected and observed hetero- zygosity and polymorphism information content per locus ranged from 0.2544 to 0.859, 0.2083 to 0.7917, and 0.2181 to 0.821, respectively. The polymorphic markers presented in this study may be useful for future analysis of the genetic diversity and population structure of farmed silver fox and wild red fox.
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Genotyping was carried out using a multiplex PCR of 20 autosomal microsatellite loci explicitly developed for the red fox.
“Genotyping was carried out using a multiplex PCR of 20 autosomal microsatellite loci (RF 21, 59, 125, 127, 131, 143, 155, 156, 162, 165, 199, 20065; VVM 219, 85, 838, 529, 189, 844, 82866), explicitly developed for the red fox.”
From Nutritional ecology of a prototypical generalist predator, the red fox (Vulpes vulpes) (2024) · cites this paper as [66]
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A Marker Set for Construction of a Genetic Map of the Silver Fox (Vulpes vulpes)
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Nutritional ecology of a prototypical generalist predator, the red fox (Vulpes vulpes)
Balestrieri, A., Gigliotti, S., Caniglia, R., Velli, E., Zambuto, F., De Giorgi, E., Mucci, N., Tremolada, P., & Gazzola, A. (2024) · Scientific Reports · doi:10.1038/s41598-024-58711-6 · Cited by 8
Metadata
- Authors
- Yan, S. Q., Bai, C. Y., Qi, S. M., Li, Y. M., Li, W. J., & Sun, J. H.
- Affiliations
- Jilin University (CN)
- Qingdao Agricultural University (CN)
- Venue
- Genetics and Molecular Research
- Publisher
- Genetics and Molecular Research
- Published
- 2015
- Volume
- 14
- Issue
- 2
- Pages
- 5890-5895
- ISSN
- 1676-5680 (ISSN-L)
- OpenAlex
- W814758414
- Cited by
- 2
- References
- 12
- Citations by year
- 2024: 1 · 2019: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genetics and Molecular Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- silver fox, red fox, microsatellite markers, genetic diversity, population structure, heterozygosity, polymorphism information content
- MeSH terms
- Alleles, Animals, Dogs, Foxes, Hair, Polymorphism, Genetic, Genetic Variation, Microsatellite Repeats
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