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Identification of Single Specimens of the Anopheles Gambiae Complex by the Polymerase Chain Reaction
Abstract
A ribosomal DNA-polymerase chain reaction (PCR) method has been developed for species identification of individuals of the five most widespread members of the Anopheles gambiae complex, a group of morphologically indistinguishable sibling mosquito species that includes the major vectors of malaria in Africa. The method, which is based on species-specific nucleotide sequences in the ribosomal DNA intergenic spacers, may be used to identify both species and interspecies hybrids, regardless of life stage, using either extracted DNA or fragments of a specimen. Intact portions of a mosquito as small as an egg or the segment of one leg may be placed directly into the PCR mixture for amplification and analysis. The method uses a cocktail of five 20-base oligonucleotides to identify An. gambiae, An. arabiensis, An. quadriannnulatus, and either An. melas in western Africa or An. melas in eastern and southern Africa.
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Items 101–119 of 119
Dabiré, K. R., Diabaté, A., Paré-Toé, L., Rouamba, J., Ouari, A., Fontenille, D., & Baldet, T. (2008) · Journal of Vector Ecology · doi:10.3376/1081-1710(2008)33[70:ytyasv]2.0.co;2 · Cited by 28
Koukpo, C. Z., Fassinou, A. J. Y. H., Ossè, R. A., Agossa, F. R., Sovi, A., Sewadé, W. T., Aboubakar, S., Assogba, B. S., Akogbeto, M. C., & Sezonlin, M. (2019) · Malaria Journal · doi:10.1186/s12936-019-2808-9 · Cited by 27
Ahoua Alou, L. P., Koffi, A. A., Adja, M. A., Assi, S. B., Kouassi, P. K., & N’Guessan, R. (2012) · Parasites & Vectors · doi:10.1186/1756-3305-5-289 · Cited by 26
Orsborne, J., Mohammed, A. R., Jeffries, C. L., Kristan, M., Afrane, Y. A., Walker, T., & Yakob, L. (2020) · Scientific Reports · doi:10.1038/s41598-020-57732-1 · Cited by 26
Masendu, H. T., Hunt, R. H., Govere, J., Brooke, B. D., Awolola, T. S., & Coetzee, M. (2004) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/j.trstmh.2003.10.006 · Cited by 25
Fournet, F., Adja, A. M., Adou, K. A., Dahoui, M. M. C., Coulibaly, B., Assouho, K. F., Zoh, D. D., Koné, M., Koné, A., Niamien, K. L., et al. (2022) · Malaria Journal · doi:10.1186/s12936-022-04295-3 · Cited by 25
First detection of Anopheles stephensi in Ghana using molecular surveillance
Afrane, Y. A., Abdulai, A., Mohammed, A. R., Akuamoah-Boateng, Y., Owusu-Asenso, C. M., Sraku, I. K., Yanney, S. A., Malm, K., & Lobo, N. F. (2023) · doi:10.1101/2023.12.01.569589 · Cited by 22
Akuoko, O. K., Dhikrullahi, S. B., Hinne, I. A., Mohammed, A. R., Owusu-Asenso, C. M., Coleman, S., Dadzie, S. K., Kyerematen, R., Boakye, D. A., Wilson, M. D., et al. (2024) · Parasites & Vectors · doi:10.1186/s13071-023-06065-9 · Cited by 20
Coleman, S., Yihdego, Y., Sherrard-Smith, E., Thomas, C. S., Dengela, D., Oxborough, R. M., Dadzie, S. K., Boakye, D., Gyamfi, F., Obiri-Danso, K., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-97138-1 · Cited by 17
Bargues, M. D., Morchón, R., Latorre, J. M., Cancrini, G., Mas-Coma, S., & Simón, F. (2006) · Parasitology Research · doi:10.1007/s00436-005-0107-6 · Cited by 16
Fahmy, N. T., Villinski, J. T., Bolay, F., Stoops, C. A., Tageldin, R. A., Fakoli, L., Okasha, O., Obenauer, P. J., & Diclaro, J. W. (2015) · Journal of Medical Entomology · doi:10.1093/jme/tjv003 · Cited by 16
Hamid-Adiamoh, M., Nwakanma, D., Assogba, B. S., Ndiath, M. O., D’Alessandro, U., Afrane, Y. A., & Amambua-Ngwa, A. (2021) · PLOS ONE · doi:10.1371/journal.pone.0241023 · Cited by 15
Larval habitat stability and productivity in two sites in Southern Ghana
Forson, A. O., Hinne, I. A., Sraku, I. K., & Afrane, Y. A. (2023) · Malaria Journal · doi:10.1186/s12936-023-04498-2 · Cited by 13
Malaria vector bionomics and transmission in irrigated and non-irrigated sites in western Kenya
Ondeto, B. M., Wang, X., Atieli, H., Orondo, P. W., Ochwedo, K. O., Omondi, C. J., Otambo, W. O., Zhong, D., Zhou, G., Lee, M.-C., et al. (2022) · Parasitology Research · doi:10.1007/s00436-022-07678-2 · Cited by 13
Hien, A. S., Soma, D. D., Somé, F. A., Namountougou, M., Poda, S. B., Ouédraogo, G. A., Diabaté, A., & Dabiré, R. K. (2021) · Journal of Medical Entomology · doi:10.1093/jme/tjaa240 · Cited by 7
Urban malaria in Accra, Ghana: transmission risk and influence of irrigated vegetable farms
Sabtiu, A. R. M., Sraku, I. K., Owusu-Asenso, C. M., Akuamoah-Boateng, Y., Doe, R. T., Boadu, E. N., Azumah, J. D., Eyeson, N. A. S., Abdulai, A., Amoateng, G., et al. (2025) · BMC Infectious Diseases · doi:10.1186/s12879-025-11403-6 · Cited by 5
“Identification of sibling species within the Anopheles gambiae s.l. complex was achieved through rDNA polymerase chain reaction (PCR) [21].”
Sabtiu, A. R. M., Hinne, I. A., Sraku, I. K., Halou, D. K., Doe, R. T., Attah, S. K., Aboagye-Antwi, F., & Afrane, Y. A. (2025) · Malaria Journal · doi:10.1186/s12936-025-05540-1 · Cited by 2
“Individual mosquitoes belonging to the An. gambiae s.l. were further discriminated by PCR according to the protocol by Scott et al. [28].”
Kroko-Djahouri, N. W. M., Guindo-Coulibaly, N., Assouho, K. F., Zoh, D. D., Tia, E., Sadia-Kacou, A. M. C., Azongnibo, K. R. M., Kpan, M. D. S., Kouadio, A. M. N., & Adja, A. M. (2024) · International Journal of Tropical Insect Science · doi:10.1007/s42690-024-01351-0 · Cited by 2
“An. gambiae s.l. is a complex of seven morphologically similar sister species, identifi - able by nucleotide replacements of rDNA (Scott et al. 1993; Riehl et al. 2011).”
Spatial temporal distribution of Anopheles mosquitoes in different ecological zones of Ghana
Abdulai, A., Owusu-Asenso, C. M., Mohammed Sabtiu, A. R., Sraku, I. K., Akuamoah-Boateng, Y., Ebuako, A. A., Awotwe, L. E., Doe, R. T., Boadu, E. N., Appiah, A. A., et al. (2026) · Gigabyte · doi:10.46471/gigabyte.175 · Cited by 1
Metadata
- Authors
- Scott, J. A., Brogdon, W. G., & Collins, F. H.
- Venue
- The American Journal of Tropical Medicine and Hygiene
- Published
- 1993-10
- Volume
- 49
- Issue
- 4
- Pages
- 520-529
- ISSN
- 0002-9637 (ISSN-L)
- OpenAlex
- W1831100553
- Cited by
- 1,659
- References
- 0
- FWCI
- 11.60
- Type
- journal article
- Language
- en
- Open access
- closed
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