The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies.
Open accessAbstract
A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum-infected blood samples, it was capable of detecting six parasites/microl of blood using DNA prepared from 25-microl blood spots on filter paper. The assay was evaluated on fingerprick blood samples collected on filter paper from 129 individuals living in a malaria-endemic area in Malaysia. Malaria prevalence by genus-specific nested PCR was 35.6% (46 of 129) compared with 28.7% (37 of 129) by microscopy. The nested PCR detected seven more malaria samples than microscopy in the first round of microscopic examination, malaria in three microscopically negative samples, six double infections identified as single infections by microscopy and one triple infection identified as a double infection by microscopy. The nested PCR assay described is a sensitive technique for collecting accurate malaria epidemiologic data. When coupled with simple blood spot sampling, it is particularly useful for screening communities in remote regions of the world.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Snounou, G., Viriyakosol, S., Zhu, X. P., Jarra, W., Pinheiro, L., do Rosario, V. E., Thaithong, S., & Brown, K. N. (1993) · Molecular and Biochemical Parasitology · doi:10.1016/0166-6851(93)90077-b · Cited by 1,591
Self-citation
Cited by
A large focus of naturally acquired Plasmodium knowlesi infections in human beings
Singh, B., Sung, L. K., Matusop, A., Radhakrishnan, A., Shamsul, S. S., Cox-Singh, J., Thomas, A., & Conway, D. J. (2004) · The Lancet · doi:10.1016/s0140-6736(04)15836-4 · Cited by 1,188
Self-citationOkell, L. C., Ghani, A. C., Lyons, E., & Drakeley, C. J. (2009) · The Journal of Infectious Diseases · doi:10.1086/644781 · Cited by 532
Hofmann, N., Mwingira, F., Shekalaghe, S., Robinson, L. J., Mueller, I., & Felger, I. (2015) · PLOS Medicine · doi:10.1371/journal.pmed.1001788 · Cited by 413
Perandin, F., Manca, N., Calderaro, A., Piccolo, G., Galati, L., Ricci, L., Medici, M. C., Arcangeletti, M. C., Snounou, G., Dettori, G., et al. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.3.1214-1219.2004 · Cited by 354
Self-citationReal-Time Fluorescence Loop Mediated Isothermal Amplification for the Diagnosis of Malaria
Lucchi, N. W., Demas, A., Narayanan, J., Sumari, D., Kabanywanyi, A., Kachur, S. P., Barnwell, J. W., & Udhayakumar, V. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013733 · Cited by 198
Metadata
- Authors
- Singh, B., Bobogare, A., Cox-Singh, J., Snounou, G., Abdullah, M. S., & Rahman, H. A.
- Venue
- The American journal of tropical medicine and hygiene
- Published
- 1999-04
- Volume
- 60
- Issue
- 4
- Pages
- 687-692
- ISSN
- 0002-9637 (ISSN-L)
- OpenAlex
- W2144029515
- Cited by
- 590
- References
- 1
- FWCI
- 5.23
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. https://app.cochat.ai/papers/10.4269/ajtmh.1999.60.687
- The paper
- Singh, B., Bobogare, A., Cox-Singh, J., Snounou, G., Abdullah, M. S., & Rahman, H. A. (1999). A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. The American journal of tropical medicine and hygiene. https://doi.org/10.4269/ajtmh.1999.60.687
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
