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Determination of ultra‐low oxygen concentrations in oxygen minimum zones by the STOX sensor
Open accessAbstract
The methods used until now have not been able to reliably resolve O 2 concentrations in oceanic oxygen minimum zones below 1–2 µmol L−1. We present a new amperometric sensor for the determination of ultra‐low O 2 concentrations under in situ conditions. The electrochemical STOX O 2 sensor contains a primary sensing cathode and a secondary cathode that, when polarized, prevents entry of O 2 into the sensor. This arrangement enables frequent in situ zero calibration and confers the sensor with a detection limit of 1‐10 nmol L−1 O 2, even during application on a Conductivity‐Temperature‐Depth (CTD) profiler at great water depths. The sensor was used during the Galathea 3 Expedition to demonstrate that the core of the oxygen minimum zone (OMZ) off Peru contained < 2 nM O 2. Application in a reactor on board demonstrated that changes in O 2 concentrations in OMZ water containing < 200 nmol L−1 O 2 could be monitored over periods of hours to days. The linear decrease in O 2 concentration in the reactor indicated very low (< 20 nmol L−1) half saturation constants for the O 2 respiring microbial community.
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Cited by
Declining oxygen in the global ocean and coastal waters
Breitburg, D., Levin, L. A., Oschlies, A., Grégoire, M., Chavez, F. P., Conley, D. J., Garçon, V., Gilbert, D., Gutiérrez, D., Isensee, K., et al. (2018) · Science · doi:10.1126/science.aam7240 · Cited by 3,253
“…of in situ oxygen concentrations ≤ 1 m mol kg − 1 has been improved by the development of STOX (switchable trace amount oxygen) sensors ( 117 ), and novel trace-oxygen optical sensors can now provide precise oxygen quantification in OMZs and detect oxygen concentrations as low as ~5…”
A Cryptic Sulfur Cycle in Oxygen-Minimum–Zone Waters off the Chilean Coast
Canfield, D. E., Stewart, F. J., Thamdrup, B., De Brabandere, L., Dalsgaard, T., Delong, E. F., Revsbech, N. P., & Ulloa, O. (2010) · Science · doi:10.1126/science.1196889 · Cited by 624
Self-citationCANFIELD, D. E., & THAMDRUP, B. (2009) · Geobiology · doi:10.1111/j.1472-4669.2009.00214.x · Cited by 479
Self-citationQuantifying the areal extent and dissolved oxygen concentrations of Archean oxygen oases
Olson, S. L., Kump, L. R., & Kasting, J. F. (2013) · Chemical Geology · doi:10.1016/j.chemgeo.2013.08.012 · Cited by 239
Fox, E. P., Cowley, E. S., Nobile, C. J., Hartooni, N., Newman, D. K., & Johnson, A. D. (2014) · Current Biology · doi:10.1016/j.cub.2014.08.057 · Cited by 207
“albicans Biofilms Create a Hypoxic Microenvironment To test the hypothesis that biofilms create locally hypoxic environments that enable the growth of anaerobic bacteria, we measured oxygen levels in biofilms using a miniaturized, switchable trace oxygen (STOX) sensor, an instrument capable ofmeasuring oxygen concentr…”
Method
Metadata
- Authors
- Revsbech, N. P., Larsen, L. H., Gundersen, J., Dalsgaard, T., Ulloa, O., & Thamdrup, B.
- Venue
- Limnology and Oceanography: Methods
- Publisher
- Wiley
- Published
- 2009-05
- Volume
- 7
- Issue
- 5
- Pages
- 371-381
- ISSN
- 1541-5856 (ISSN-L)
- OpenAlex
- W1523470136
- Cited by
- 268
- References
- 0
- FWCI
- 9.42
- Type
- journal article
- Language
- en
- Open access
- green
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- Revsbech, N. P., Larsen, L. H., Gundersen, J., Dalsgaard, T., Ulloa, O., & Thamdrup, B. (2009). Determination of ultra‐low oxygen concentrations in oxygen minimum zones by the STOX sensor. Limnology and Oceanography: Methods. https://doi.org/10.4319/lom.2009.7.371
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