Relationship Between Pigment Concentration and Dry Weight in Determining Microalgae Abundance in Artificial Water Samples
Open accessAbstract
Microalgae are aquatic organisms that can function as bioindicators of surface water quality. The concentration of chlorophyll a can be measured to determine the level of microalgae abundance in a body of water. Measurement of the dry weight of microalgae biomass is another method that can be used to determine the abundance of microalgae in a body of water. Both methods have their advantages and disadvantages. In measuring chlorophyll concentration, the presence of other compounds besides chlorophyll which can absorb light spectrum at certain wavelengths (wavelengths of 665 nm, 645 nm, and 630 nm) causes the measured absorbance value to be greater than it should be. The level of turbidity due to the content of suspended particles in water is a problem in the dry weight measurement method. Determination of the dry weight of biomass based on the chlorophyll concentration approach is the subject of this study. The results of simple linear regression analysis showed that the concentration of chlorophyll a and the dry weight of microalgae biomass in treatment A had a fairly strong positive correlation (Ra = 0.870), the tendency of which was to follow the linear regression equation Y = 302.35x + 17.121. Determination of dry weight based on the chlorophyll concentration measurement approach can be applied to water samples with suspended solids content that tends to be constant and inert (does not produce substances that can react with organic solvents during the chlorophyll extraction process). In addition, based on the results of data processing it can be concluded that the effect of the content of other suspended particles in the water samples did not show a statistically significant effect.
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Chlorophyll demonstrates a direct proportionality to growth (cell weight) under conditions unaffected by external factors, such as light stress, temperature changes, or the nutrient content of the medium.
“Chlorophyll demonstrates a direct proportionality to growth (cell weight) under conditions unaffected by external factors, such as light stress, temperature changes, or the nutrient content of the medium”
From Quantitative Prediction of Spirulina platensis Biomass Using UV-Vis Spectrophotometry (2026) · cites this paper as Eddiwan et al., 2023
The Conclusion (page not indicated) reports a fairly close positive correlation between chlorophyll concentration and microalgae dry weight, supporting the general relationship. It does not establish direct proportionality or examine whether that relationship holds in the absence of light stress, temperature changes, or nutrient differences; its stated applicability condition concerns constant, inert suspended solids. (LLM, conf=0.86)
Experimental — accuracy not yet measuredBecause pigments do not absorb light at 750 nm, this wavelength is used to assess apparent turbidity through light scattering.
“Finally, because pigments do not absorb light at 750 nm, this wavelength is used to assess apparent turbidity through light scattering”
From Quantitative Prediction of Spirulina platensis Biomass Using UV-Vis Spectrophotometry (2026) · cites this paper as Eddiwan et al., 2023
The 'Determination of Microalgae Content' section says the standard method uses absorbance at 750 nm to measure the contribution of suspended particles other than microalgae, which supports the wavelength’s role as a turbidity-related measure. It does not state that pigments do not absorb at 750 nm or identify light scattering as the mechanism. It also says the authors did not take a 750-nm measurement in this experiment; no page number is available in the indexed passages. (LLM, conf=0.86)
Experimental — accuracy not yet measured
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Sorted by citations
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Quantitative Prediction of Spirulina platensis Biomass Using UV-Vis Spectrophotometry
Lestari, F. S., Zulys, A., & Satya, A. (2026) · LIMNOTEK Perairan Darat Tropis di Indonesia · doi:10.55981/limnotek.2026.14459
“Chlorophyll demonstrates a direct proportionality to growth (cell weight) under conditions unaffected by external factors, such as light stress, temperature changes, or the nutrient content of the medium (Hotos et al., 2020; Fakhri et al., 2021; Eddiwan et al., 2023).”
- Authors
- Eddiwan, K., Dahril, T., & Efawani, E.
- Affiliations
- Riau University (ID)
- Venue
- International Journal of Research and Scientific Innovation
- Publisher
- RSIS International
- Published
- 2023
- Volume
- X
- Issue
- V
- Pages
- 118-127
- ISSN
- 2320-5520 (ISSN-L)
- 2321-2705
- OpenAlex
- W4381335660
- Cited by
- 1
- References
- 34
- Citations by year
- 2025: 1
- FWCI
- 0.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- International journal of research and scientific innovation (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Environmental Science › Waste Management and Disposal › Water Quality Monitoring and Analysis
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal ecosystems
- Keywords
- chlorophyll a, bioindicators, surface water quality, turbidity, suspended solids, chlorophyll extraction, biomass estimation
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