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Cited by 5Antioxidant Activity and Oxidative StressNitric Oxide and Endothelin EffectsAdvanced Glycation End Products research

Predictive value of some pro-oxidants in type 2 diabetes mellitus with vascular complications

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Goycheva, Petia, Nikolova, Galina, Ivanova, Mariana, Kundurdzhiev, Todor, Gadjeva, Veselina·2019·BioScience Trends
DOI 10.5582/bst.2019.01020
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Abstract

The study aims to analyze oxidative stress levels in circulation of some reactive molecules and products of biomolecular modification in type 2 diabetes mellitus (T2DM) with diabetes-specific vascular complications in order to determine their predictive value. Also, the alterations of their serum concentration with reference to disease characteristics were assessed. Reactive oxygen species (ROS), nitric oxide radicals (•NO), malondialdehyde (MDA), protein carbonyl (CO) and 8-hydroxydeoxyguanosin (8-OHdG) in serum were measured in 93 patients with T2DM with vascular complications, 94 control subjects and 16 diabetic patients who had no evidence of vascular disease. T2DM patients with clinically manifest vascular disease exhibit significantly elevated concentrations of all pro-oxidants in comparison to healthy subjects, with the highest degree of increase of •NO radicals. The levels of carbonylated proteins, ROS and 8-OHdG were significantly increased in insufficiently compensated diabetes as compared to good glycemic control state. Also, serum MDA, protein CO and 8-OHdG showed an association with glycemic control parameters. MDA, ROS and 8-OHdG correlated mostly with microvascular complications. Significant area under the curve (AUC) from plotted receiver operating characteristic (ROC) curves were obtained for all studied biomarkers, as for nitric oxide it was substantially bigger compared to those for the other pro-oxidants. Correspondingly, positive and negative predictive values related to the disease were in favor of the •NO radicals. The cutoff values of oxidative biomarkers may serve as an indicator of clinical reference for detecting T2DM with associated vascular complications, as nitric oxide radicals were the most reliable indicator.

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  • Plasma MDA levels are higher in T2D patients with vascular complications (n = 93) than in healthy individuals (n = 94), as measured by TBARS assay.

    “| T2D with vascular complications | Plasma | Healthy individuals (n = 94) vs. T2D patients with vascular complications(n = 93) | TBARS assay | Higher levels of MDA in T2D patients with vascular complications | [149] |”

    From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [149]

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Authors
Goycheva, P., Nikolova, G., Ivanova, M., Kundurdzhiev, T., & Gadjeva, V.
Affiliations
  • Clinic of Endocrinology, University Hospital "Prof. Dr. Stoyan Kirkovich", Medical Faculty, Trakia University
  • Trakia University (BG)
  • University Hospital St. Ivan Rilski (BG)
  • Faculty of Public Health, Medical University
ORCID
Venue
BioScience Trends
Publisher
International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA)
Published
2019-04-30
Volume
13
Issue
2
Pages
168-175
ISSN
  • 1881-7815 (ISSN-L)
  • 1881-7823
DOI
10.5582/bst.2019.01020
OpenAlex
W2935034332
Cited by
5
References
1
Citations by year
2026: 1 · 2022: 1 · 2021: 3
FWCI
0.61
Type
journal article
Language
en
Open access
Locations
  • BioScience Trends (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
Keywords
type 2 diabetes, diabetic vascular complications, oxidative stress, pro-oxidants, reactive oxygen species, nitric oxide radical, malondialdehyde, protein carbonylation, 8-hydroxy-2'-deoxyguanosine, glycemic control, microvascular complications, biomarkers, predictive value
MeSH terms
Adult, Aged, Aged, 80 and over, Diabetes Mellitus, Type 2, Diabetic Angiopathies, Female, Humans, Male, Middle Aged, Predictive Value of Tests, ROC Curve, Cohort Studies, Biomarkers, Case-Control Studies, Oxidants

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