Investigation of the lipid changes that occur in hypertrophic muscle due to fish protein-feeding using mass spectrometry imaging
Open accessAbstract
Alaska pollack protein (APP) was previously shown to reduce serum triacylglycerol and the atherogenic index and significantly increase gastrocnemius muscle mass in rats. To determine which myofibers are involved in this observed hypertrophy, we stained the gastrocnemius muscle with fast and slow fiber-specific antibodies and measured the muscle fiber diameter. We observed muscle hypertrophy in both the fast and slow fibers of APP-fed rats. Although muscle hypertrophy leads to drastic lipid changes, the amount of lipids did not differ significantly between casein-fed and APP-fed rats. To determine the lipid changes at the molecular species level and their localization, we performed matrix-assisted laser desorption/ionization mass spectrometry imaging to visualize lipids in the gastrocnemius muscles. We determined that lipid molecules were significantly changed due to APP feeding. Thus, APP feeding changes muscle lipid metabolism, and these metabolic changes might be related to hypertrophy.
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Ingestions of APP for 6 or 8 weeks induce significant increases in the gastrocnemius muscle weight and fiber diameters when comparing with ingestion of CAS in rats.
“The previous study demonstrated that ingestions of APP for 6 or 8 weeks induce significant increases in the gastrocnemius muscle weight and fiber diameters when comparing with ingestion of CAS (14–16) and a significant increase of myosin heavy-chain gene expression in Myh4 (14) in rats.”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [16]
The Results report significantly larger gastrocnemius fiber diameters in APP-fed rats than in casein-fed rats, and the paper reports increased gastrocnemius muscle mass with APP feeding. However, the Animals section describes a 7-day feeding experiment, not 6 or 8 weeks; this source does not establish the claimed duration. (LLM, conf=0.90)
Experimental — accuracy not yet measuredMorisasa and colleagues reported that lipid molecules were significantly changed in APP-fed rats than in CAS-fed rats.
“Also Mizushige and colleagues (15) showed decrease in serum triglycerides and inhibition of visceral body fat accumulation in rats, and Morisasa and colleagues reported that lipid molecules were significantly changed in APP-fed rats than in CAS-fed rats (16).”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [16]
Alaska pollack protein (APP) ingestion induced more marked muscle hypertrophy compared with casein (CAS) in rat muscle.
“Mizushige and colleagues (14), Kawabata and colleagues (15), and Morisasa and colleagues (16) showed that Alaska pollack protein (APP) ingestion induced more marked muscle hypertrophy comparing with casein (CAS) in rat muscle (14–16).”
From Modulation of Neural and Muscular Adaptation Processes During Resistance Training by Fish Protein Ingestions in Older Adults (2020) · cites this paper as [16]
In 5-week-old male Sprague-Dawley rats, the volume of gastrocnemius increased in Alaska pollack protein-fed rats compared to casein-fed animals, with volume increase in both fast and slow fibers.
“a recent study using 5-week-old male Sprague-Dawley rats showed that the volume of gastrocnemius increased in the APP-fed rats in comparison to the casein-fed animals. The results indicated a volume increase in both fast and slow fibers [50].”
From Novel Insights on Intake of Fish and Prevention of Sarcopenia: All Reasons for an Adequate Consumption (2020) · cites this paper as [50]
Muscular atrophy and hypertrophy mainly affect fast fibers.
“Muscular atrophy and hypertrophy mainly affect fast fibers [50].”
From Novel Insights on Intake of Fish and Prevention of Sarcopenia: All Reasons for an Adequate Consumption (2020) · cites this paper as [50]
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References 13 · Cited by 16
- Authors
- Morisasa, M., Goto-Inoue, N., Sato, T., Machida, K., Fujitani, M., Kishida, T., Uchida, K., & Mori, T.
- Affiliations
- Nihon University (JP)
- Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University
- Ehime University (JP)
- Nippon Suisan Kaisha (Japan) (JP)
- Venue
- Journal of Oleo Science
- Publisher
- Japan Oil Chemists' Society
- Published
- 2019
- Volume
- 68
- Issue
- 2
- Pages
- 141-148
- ISSN
- 1341-8327 (ISSN-L)
- 1345-8957
- 1347-3352
- OpenAlex
- W2913232529
- Cited by
- 16
- References
- 13
- Citations by year
- 2024: 3 · 2023: 5 · 2022: 3 · 2021: 3 · 2020: 1 · 2019: 1
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Oleo Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Keywords
- muscle hypertrophy, gastrocnemius, muscle lipid metabolism, lipid species, fast and slow muscle fibers, muscle fiber diameter, serum triglycerides, atherogenic index, casein diet, lipid localization
- MeSH terms
- Animals, Hypertrophy, Lipids, Male, Rats, Sprague-Dawley, Muscle, Skeletal, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Fish Proteins, Gadiformes, Lipid Metabolism
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