Identification and Antimicrobial Activity Detection of Lactic Acid Bacteria Isolated from Corn Stover Silage
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A total of 59 lactic acid bacteria (LAB) strains were isolated from corn stover silage. According to phenotypic and chemotaxonomic characteristics, 16S ribosomal DNA (rDNA) sequences and recA gene polymerase chain reaction amplification, these LAB isolates were identified as five species: Lactobacillus (L.) plantarum subsp. plantarum, Pediococcus pentosaceus, Enterococcus mundtii, Weissella cibaria and Leuconostoc pseudomesenteroides, respectively. Those strains were also screened for antimicrobial activity using a dual-culture agar plate assay. Based on excluding the effects of organic acids and hydrogen peroxide, two L. plantarum subsp. plantarum strains ZZU 203 and 204, which strongly inhibited Salmonella enterica ATCC 43971(T), Micrococcus luteus ATCC 4698(T) and Escherichia coli ATCC 11775(T) were selected for further research on sensitivity of the antimicrobial substance to heat, pH and protease. Cell-free culture supernatants of the two strains exhibited strong heat stability (60 min at 100°C), but the antimicrobial activity was eliminated after treatment at 121°C for 15 min. The antimicrobial substance remained active under acidic condition (pH 2.0 to 6.0), but became inactive under neutral and alkaline condition (pH 7.0 to 9.0). In addition, the antimicrobial activities of these two strains decreased remarkably after digestion by protease K. These results preliminarily suggest that the desirable antimicrobial activity of strains ZZU 203 and 204 is the result of the production of a bacteriocin-like substance, and these two strains with antimicrobial activity could be used as silage additives to inhibit proliferation of unwanted microorganism during ensiling and preserve nutrients of silage. The nature of the antimicrobial substances is being investigated in our laboratory.
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The epiphytic LAB number of corn stover used in this study was 9.24 × 10^9 cfu/kg FM, much higher than that of corn stover in the studies of Liu et al. (3.47 × 10^8 cfu/kg FM) and Li et al. (1.4 × 10^8 cfu/kg FM).
“The epiphytic LAB number of corn stover used in this study was 9.24 × 109 cfu/kg FM, much higher than that of corn stover in study of Liu et al. (3.47 × 108 cfu/kg FM)14 and Li et al. (1.4 × 108 cfu/kg FM)30.”
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References 34 · Cited by 92
- Authors
- Li, D., Ni, K., Pang, H., Wang, Y., Cai, Y., & Jin, Q.
- Affiliations
- Zhengzhou University (CN)
- Institute of Livestock and Grassland Science (JP)
- ZheJiang Academy of Agricultural Sciences (CN)
- Corresponding author
- Wang, Yanping
- Venue
- Asian-Australasian Journal of Animal Sciences
- Published
- 2015-02-06
- Volume
- 28
- Issue
- 5
- Pages
- 620-631
- ISSN
- 1011-2367 (ISSN-L)
- 1976-5517
- PMC
- PMC4412991
- OpenAlex
- W2073487358
- Cited by
- 92
- References
- 34
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 9 · 2023: 9 · 2022: 11 · 2021: 12 · 2020: 18 · 2019: 7 · 2018: 8 · 2017: 6 · 2016: 2
- FWCI
- 7.14
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Asian-Australasian Journal of Animal Sciences (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (41101244, 201103007)
- Special Fund for Agro-scientific Research in the Public Interest (201103007)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- lactic acid bacteria, corn stover silage, antimicrobial activity, bacteriocin-like inhibitory substances, silage additives, thermal stability, pH stability, protease sensitivity, 16S rRNA identification, recA gene
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