Association analysis of polymorphisms in six keratin genes with wool traits in sheep
Open accessAbstract
Objective: The purpose of this study was to investigate the genetic effects of six keratin (KRT) genes on the wool traits of 418 Chinese Merino (Xinjiang type) (CMXT) individuals.Methods: To explore the effects and association of six KRT genes on sheep wool traits, The polymerase chain reaction-based single-strand conformation polymorphism (PCR-SSCP), DNA sequencing, and the gene pyramiding effect methods were used.Results: We report 20 mutation sites (single-nucleotide polymorphisms) within the six KRT genes, in which twelve induced silent mutations; five induced missense mutations and re sulted in Ile→Thr, Glu→Asp, Gly→Ala, Ala→Ser, Se→His; two were nonsense mutations and one was a same-sense mutation.Association analysis showed that two genotypes of the KRT31 gene were significantly associated with fiber diameter (p<0.05);three genotypes of the KRT36 gene were significantly associated with wool fineness score and fiber diameter (p<0.05),three genotypes of the KRT38 gene were significantly associated with the number of crimps (p< 0.05); and three genotypes of the KRT85 gene were significantly associated with wool crimps score, body size, and fiber diameter (p<0.05).Analysis of the gene pyramiding effect between the different genotypes of the gene loci KRT36, KRT38, and KRT85, each genotype in a gene locus was combined with all the genotypes of another two gene loci and formed the different three loci combinations, indicated a total of 26 types of possible combined genotypes in the analyzed population.Compared with the other combined genotypes, the combinations CC-GG-II, CC-HH-IJ, CC-HH-JJ, DD-HH-JJ, CC-GH-IJ, and CC-GH-JJ at gene loci KRT36, KRT38, and KRT85, respectively, had a greater effect on wool traits (p<0.05).Conclusion: Our results indicate that the mutation loci of KRT31, KRT36, KRT38, and KRT85 genes, as well as the combinations at gene loci KRT36, KRT38, and KRT85 in CMXT have significant effects on wool traits, suggesting that these genes are important candidate genes for wool traits, which will contribute to sheep breeding and provide a molecular basis for improved wool quality in sheep.
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Variants of keratin and keratin-associated protein genes have been shown to associate with wool quality and are potential selection markers for livestock improvements.
“Importantly, variants of these genes have been shown to associate with wool quality, and are potential selection markers for livestock improvements164–167.”
From Chromosomal-level reference genome assembly of muskox (Ovibos moschatus) from Banks Island in the Canadian Arctic, a resource for conservation genomics (2024) · cites this paper as [165]
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- Authors
- Sulayman, A., Tursun, M., Sulaiman, Y., Huang, X., Tian, K., Tian, Y., Xu, X., Fu, X., Mamat, A., & Tulafu, H.
- Affiliations
- Xinjiang Agricultural University (CN)
- Xinjiang Academy of Animal Science (CN)
- Corresponding author
- Huang, Xixia
- Tian, Kechuan
- Venue
- Asian-Australasian Journal of Animal Sciences
- Published
- 2018-06-01
- Volume
- 31
- Issue
- 6
- Pages
- 775-783
- ISSN
- 1011-2367 (ISSN-L)
- 1976-5517
- PMC
- PMC5933973
- OpenAlex
- W2766154774
- Cited by
- 31
- References
- 30
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 9 · 2023: 3 · 2022: 4 · 2021: 2 · 2020: 5 · 2019: 3
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Asian-Australasian Journal of Animal Sciences (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Modern Agriculture Industrial System Project (CARS-40)
- National Natural Science Foundation of China (31360543, 31460593)
- Xinjiang Uygur Autonomous Region science and technology major projects (201230116-6)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
- Keywords
- keratin genes, Chinese Merino sheep, single nucleotide polymorphisms, gene pyramiding, fiber diameter, wool fineness, fiber crimp, candidate genes, genetic association, missense mutations, nonsense mutation, body size
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