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Cited by 14Mesenchymal stem cell researchLiver physiology and pathologyLiver Disease and Transplantation

The DOI matches its Crossref record, with a small difference in title or year; 1 of the 1 references it cites were found in a registry or an index.

Transplant of Hepatocytes, Undifferentiated Mesenchymal Stem Cells, and In Vitro Hepatocyte-Differentiated Mesenchymal Stem Cells in a Chronic Liver Failure Experimental Model: A Comparative Study.

Hanan El Baz, Zeinab A. Demerdash, Manal M. Kamel, Shimaa Attia Atta, Faten Salah, Salwa Hassan, Olfat Ali Hammam, Heba M. A. Khalil, Safa S. Meshaal, Inas Ismail Raafat·2017·Experimental and Clinical Transplantation
DOI 10.6002/ect.2016.0226
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Abstract

OBJECTIVES: Liver transplant is the cornerstone line of treatment for chronic liver diseases; however, the long list of complications and obstacles stand against this operation. Searching for new modalities for treatment of chronic liver illness is a must. In the present research, we aimed to compare the effects of transplant of undifferentiated human mesenchymal stem cells, in vitro differentiated mesenchymal stem cells, and adult hepatocytes in an experimental model of chronic liver failure. MATERIALS AND METHODS: Undifferentiated human cord blood mesenchymal stem cells were isolated, pro-pagated, and characterized by morphology, gene expression analysis, and flow cytometry of surface markers and in vitro differentiated into hepatocyte-like cells. Rat hepatocytes were isolated by double perfusion technique. An animal model of chronic liver failure was developed, and undifferentiated human cord blood mesenchymal stem cells, in vitro hepato-genically differentiated mesenchymal stem cells, or freshly isolated rat hepatocytes were transplanted into a CCL4 cirrhotic experimental model. Animals were killed 3 months after transplant, and liver functions and histopathology were assessed. RESULTS: Compared with the cirrhotic control group, the 3 cell-treated groups showed improved alanine aminotransferase, aspartate aminotransferase, albumin, and bilirubin levels, with best results shown in the hepatocyte-treated group. Histopathologic examination of the treated groups showed improved fibrosis, with best results obtained in the undifferentiated mesenchymal stem cell-treated group. CONCLUSIONS: Both adult hepatocytes and cord blood mesenchymal stem cells proved to be promising candidates for cell-based therapy in liver regeneration on an experimental level. Improved liver function was evident in the hepatocyte-treated group, and fibrosis control was more evident in the undifferentiated mesenchymal stem cell-treated group.

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Metadata

Authors
Baz, H. E., Demerdash, Z. A., Kamel, M. M., Atta, S. A., Salah, F., Hassan, S., Hammam, O. A., Khalil, H. M. A., Meshaal, S. S., & Raafat, I. I.
Affiliations
Theodor Bilharz Research Institute (EG)
ORCID
Venue
Experimental and Clinical Transplantation
Publisher
Baskent University
Published
2018-02
Volume
16
Issue
1
Pages
81-89
ISSN
  • 1304-0855 (ISSN-L)
  • 2146-8427
DOI
10.6002/ect.2016.0226
OpenAlex
W3022572308
Cited by
14
References
1
Citations by year
2025: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 4 · 2019: 3 · 2018: 1
Type
paratext
Language
en
Open access
closed
Locations
  • Experimental and Clinical Transplantation (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Hepatology › Liver Disease and Transplantation
Keywords
mesenchymal stem cells, hepatocyte transplantation, chronic liver failure, liver cirrhosis, carbon tetrachloride-induced liver injury, liver regeneration, liver fibrosis, cell therapy, cord blood stem cells, rat model
MeSH terms
Animals, Carbon Tetrachloride, Cell Differentiation, Cells, Cultured, Humans, Liver Cirrhosis, Experimental, Liver Regeneration, Male, Mice, Inbred BALB C, Phenotype, Rats, Inbred Lew, Time Factors, Biomarkers, Liver Transplantation, Recovery of Function, Hepatocytes, Cord Blood Stem Cell Transplantation, Mesenchymal Stem Cell Transplantation, Chemical and Drug Induced Liver Injury, End Stage Liver Disease, Mesenchymal Stem Cells

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