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Cited by 128Cellular transport and secretionCaveolin-1 and cellular processesAutophagy in Disease and Therapy

Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits

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Messa, Mirko, Fernández-Busnadiego, Rubén, Sun, Elizabeth Wen, Chen, Hong, Czapla, Heather, Wrasman, Kristie, Wu, Yumei, Ko, Genevieve +3 more·2014·eLife
DOI 10.7554/elife.03311PMC PMC4161027
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Abstract

Epsin is an evolutionarily conserved endocytic clathrin adaptor whose most critical function(s) in clathrin coat dynamics remain(s) elusive. To elucidate such function(s), we generated embryonic fibroblasts from conditional epsin triple KO mice. Triple KO cells displayed a dramatic cell division defect. Additionally, a robust impairment in clathrin-mediated endocytosis was observed, with an accumulation of early and U-shaped pits. This defect correlated with a perturbation of the coupling between the clathrin coat and the actin cytoskeleton, which we confirmed in a cell-free assay of endocytosis. Our results indicate that a key evolutionary conserved function of epsin, in addition to other roles that include, as we show here, a low affinity interaction with SNAREs, is to help generate the force that leads to invagination and then fission of clathrin-coated pits.

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  • Epsin1 has been implicated in endocytosis at synapses and can interact with both the plasma membrane and F-actin through its N-terminal ENTH domain and C-terminal tail, respectively.

    “Epsin1 has been implicated in endocytosis at synapses71 and can interact with both the plasma membrane and F-actin through its N-terminal ENTH domain and C-terminal tail, respectively (Supplementary Fig. 7a)72.”

    From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [72]

  • Epsin1 interacts with other endocytic proteins such as Eps15 and Hip1R, both of which interact either directly or indirectly with membranes and actin.

    “However, it is not clear whether Epsin1 is the sole organizer of F-actin at synapses, since it interacts with other endocytic proteins such as Eps15 and Hip1R72,88, both of which interact either directly or indirectly with membranes and actin.”

    From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [72]

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Authors
Messa, M., Fernández-Busnadiego, R., Sun, E. W., Chen, H., Czapla, H., Wrasman, K., Wu, Y., Ko, G., Ross, T., Wendland, B., & De Camilli, P.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Program in Cellular Neuroscience, Neurodegeneration and Repair, Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, United States
  • Johns Hopkins University (US)
  • The University of Texas Southwestern Medical Center (US)
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2014-08-13
Volume
3
Pages
e03311-e03311
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.03311
PMC
PMC4161027
OpenAlex
W2070297191
Cited by
128
References
2
Citations by year
2026: 5 · 2025: 8 · 2024: 2 · 2023: 3 · 2022: 14 · 2021: 19 · 2020: 21 · 2019: 10 · 2018: 15 · 2017: 8 · 2016: 14 · 2015: 8 · 2014: 1
FWCI
5.15
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
Funders
  • Howard Hughes Medical Institute
  • National Institute of Neurological Disorders and Stroke (R37NS036251)
  • National Institute on Drug Abuse (DA018343)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK082700, DK45735)
  • National Institute of General Medical Sciences (R01GM60979, NIGMST32007231)
  • National Cancer Institute (R01CA098730)
  • American Heart Association (0835544N)
  • Alexander von Humboldt-Stiftung
  • National Institutes of Health (5T32GM007223-38/39)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
epsin, clathrin-mediated endocytosis, actin cytoskeleton, clathrin adaptor proteins, cell division defects, embryonic fibroblasts
MeSH terms
Actins, Animals, Clathrin, Coated Pits, Cell-Membrane, Embryo, Mammalian, Endocytosis, Fibroblasts, Actin Cytoskeleton, Protein Binding, Signal Transduction, Gene Expression, Mice, Knockout, Adaptor Proteins, Vesicular Transport, SNARE Proteins, Mice, Primary Cell Culture

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