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Cited by 0Glycosylation and Glycoproteins ResearchCellular transport and secretionViral Infectious Diseases and Gene Expression in Insects

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Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner

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Kaizuka, Yoshihisa, Machida, Rika·2025·eLife
DOI 10.7554/elife.101175PMC PMC12563552

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Abstract

Various types of glycoproteins have been suggested to inhibit viral infection of cells via steric repulsion. However, it is difficult to evaluate such physical actions genetically, simply because they are nonspecific and can be caused by any molecule. Therefore, we investigated a method to compare this nonspecific action among cells with diverse membrane protein profiles. We found that a wide range of glycoproteins individually had a strong inhibitory effect on infection, while on the other hand, the total amount of glycans was negatively correlated with the infection level in each cell. Thus, the infection-inhibitory effect of glycoproteins was molecularly nonspecific but was additively enhanced according to the amount of glycans on the cell surface. In this correlation, glycans function as a fundamental factor. Further investigating the mechanism by which glycans function as a factor in infection control, we conclude that the repulsion between proteins created by branched glycans forms a kinetic energy barrier against packing the virus into the region of protein interstitial space. As a result, the formation of the adhesive interface between the virus and the cell membrane, which is necessary for infection, is inhibited. This study attempted to link the cell’s nonspecific physical properties with intracellular biochemicals. A similar approach may be applied to quantify other nonspecific biological phenomena.

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Metadata

Authors
Kaizuka, Y., & Machida, R.
Affiliations
National Institute for Materials Science (JP)
Corresponding author
Kaizuka, Yoshihisa
ORCID
Kaizuka, Yoshihisa
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2025-10-28
Volume
14
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.101175
PMC
PMC12563552
OpenAlex
W4406518185
Cited by
0
References
3
Type
posted content
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • NIMS Materials Data Repository (submittedVersion, cc-by)
Funders
National Institute for Materials Science
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
viral infection inhibition, steric repulsion, glycoproteins, kinetic barriers, cell surface glycosylation
MeSH terms
Animals, Cell Membrane, Glycoproteins, Humans, Kinetics, Polysaccharides, Virion, Virus Internalization

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