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Cited by 0Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsNuclear Structure and Function

The DOI matches its Crossref record, with a small difference in title or year; 5 of the 5 references it cites were found in a registry or an index.

The chromokinesin Kid (KIF22) forms a homodimer, moves processively along microtubules, and transports double-stranded DNA

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Niwa, Shinsuke, Furusaki, Natsuki, Kita, Tomoki, Suzuki, Yuki, Chiba, Kyoko·2026·eLife
DOI 10.7554/elife.102828PMC PMC13581288

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Abstract

During prometaphase in mitosis, chromosomes are pushed toward the spindle equator. The chromokinesin Kid, also known as KIF22, moves chromosomes along spindle microtubules during prometaphase. Kid has long been considered a monomeric and nonprocessive motor, different from typical kinesins. In this study, we demonstrate that the full-length Kid forms a homodimer and moves processively along microtubules. A conserved coiled-coil domain within the stalk region of Kid is sufficient for homodimer formation and is required for the processivity of Kid. Furthermore, the neck linker and coiled-coil domains of Kid could add processive activity to the motor domain of KIF1A, suggesting that Kid contains a functional neck linker and dimerization capability, a prerequisite for the processivity of kinesin motor domains. The full-length Kid, containing a helix–hairpin–helix domain, can transport double-stranded DNA along microtubules in vitro. AlphaFold3 prediction suggests that the dimerization of Kid stabilizes the association with DNA. These findings collectively suggest the reclassification of Kid as a processive and dimeric motor that transports DNA along microtubules.

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Metadata

Authors
Niwa, S., Furusaki, N., Kita, T., Suzuki, Y., & Chiba, K.
Affiliations
  • Tohoku University (JP)
  • Mie University (JP)
Corresponding author
  • Niwa, Shinsuke
  • Chiba, Kyoko
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2026-09-16
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.102828
PMC
PMC13581288
OpenAlex
W4404523437
Cited by
0
References
5
Type
posted content
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Japan Society for the Promotion of Science (JP23H02472, JP23KJ0168, JP22K15053)
  • Uehara Memorial Foundation
  • Naito Foundation
  • Takeda Science Foundation (2024036450)
  • Astellas Foundation for Research on Metabolic Disorders (2024a1048)
  • Brain Science Foundation
  • MEXT Leading Initiative for Excellent Researchers (JPMXS0320200156)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
KIF22, chromokinesin, homodimerization, microtubule motility, coiled-coil domain, neck linker, DNA transport, double-stranded DNA, mitotic spindle
MeSH terms
Protein Domains, Animals, DNA, DNA-Binding Proteins, Humans, Microtubules, Kinesins, Protein Multimerization

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