Highly processive microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains
Abstract
Studies of immobilized kinesin have shown that a single dimeric molecule can maintain contact with and drive sliding of a microtubule. In solution, however, native kinesin binds microtubules too weakly and hydrolyses ATP too slowly to produce the high sliding velocities seen in motility assay. This apparent inhibition in solution appears to be caused by the binding of kinesin's tail domains to its motor (head) domains in a folded conformation. DKH392, a construct containing two heads but no tails, has been shown to display both tight binding to microtubules and high ATPase rates. Furthermore, it retains one molecule of ADP per dimer when bound to microtubules, which could facilitate a 'hand-over-hand' mechanism for processive motion. Here we show that DKH392 hydrolyses more than 100 ATP molecules per diffusional encounter with a microtubule, even in the high-salt conditions encountered physiologically. This provides direct evidence that kinesin's activity is highly processive, with the motor remaining attached to a microtubule through many cycles of ATP hydrolysis.
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The kcat value of Pkl1 is smaller than that reported for kinesin-1 (~50 s−1) but similar to those of Ncd, Kar3, and Eg5.
“the k cat value was smaller than that reported for kinesin-1 (ϳ50 s Ϫ1 ) (33,34) but was similar to those of Ncd, Kar3, and Eg5.”
From Diffusion and Directed Movement (2008) · cites this paper as [33]
Cites
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
VALE, R., REESE, T., & SHEETZ, M. (1985) · Cell · doi:10.1016/s0092-8674(85)80099-4 · Cited by 1,993 · see citing works
Direct observation of kinesin stepping by optical trapping interferometry
Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. (1993) · Nature · doi:10.1038/365721a0 · Cited by 1,910 · see citing works
Bead movement by single kinesin molecules studied with optical tweezers
Block, S. M., Goldstein, L. S. B., & Schnapp, B. J. (1990) · Nature · doi:10.1038/348348a0 · Cited by 1,038 · see citing works
Movement of microtubules by single kinesin molecules
Howard, J., Hudspeth, A. J., & Vale, R. D. (1989) · Nature · doi:10.1038/342154a0 · Cited by 885 · see citing works
Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.
Hackney, D. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.6865 · Cited by 351 · see citing works
Self-citationPathway of processive ATP hydrolysis by kinesin
Gilbert, S. P., Webb, M. R., Brune, M., & Johnson, K. A. (1995) · Nature · doi:10.1038/373671a0 · Cited by 250 · see citing works
Kinesin ATPase: rate-limiting ADP release
Hackney, D. D. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.17.6314 · Cited by 239 · see citing works
Self-citationKinesin undergoes a 9 S to 6 S conformational transition
Hackney, D. D., Levitt, J. D., & Suhan, J. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42499-4 · Cited by 175 · see citing works
Self-citationHuang, T. G., & Hackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34033-4 · Cited by 174 · see citing works
Self-citationHuang, T. G., Suhan, J. P., & Hackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34034-6 · Cited by 90 · see citing works
Self-citationHackney, D. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34035-8 · Cited by 68 · see citing works
Self-citationA kinetic study of the kinesin ATPase.
Sadhu, A., & Taylor, E. W. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49917-1 · Cited by 59 · see citing works
Nucleotide-free Kinesin Hydrolyzes ATP with Burst Kinetics
Hackney, D. D., Malik, A. S., & Wright, K. W. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)71570-6 · Cited by 40 · see citing works
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Erbaş-Çakmak, S., Leigh, D. A., McTernan, C. T., & Nussbaumer, A. L. (2015) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00146 · Cited by 1,983 · see citing works
Schliwa, M., & Woehlke, G. (2003) · Nature · doi:10.1038/nature01601 · Cited by 1,069 · see citing works
Kinesin hydrolyses one ATP per 8-nm step
Schnitzer, M. J., & Block, S. M. (1997) · Nature · doi:10.1038/41111 · Cited by 771 · see citing works
Direct observation of single kinesin molecules moving along microtubules
Vale, R. D., Funatsu, T., Pierce, D. W., Romberg, L., Harada, Y., & Yanagida, T. (1996) · Nature · doi:10.1038/380451a0 · Cited by 711 · see citing works
Force production by single kinesin motors
Schnitzer, M. J., Visscher, K., & Block, S. M. (2000) · Nature Cell Biology · doi:10.1038/35036345 · Cited by 572 · see citing works
Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics
Hirokawa, N., & Noda, Y. (2008) · Physiological Reviews · doi:10.1152/physrev.00023.2007 · Cited by 510 · see citing works
Molecular motors: structural adaptations to cellular functions
Howard, J. (1997) · Nature · doi:10.1038/39247 · Cited by 489 · see citing works
A Processive Single-Headed Motor: Kinesin Superfamily Protein KIF1A
Okada, Y., & Hirokawa, N. (1999) · Science · doi:10.1126/science.283.5405.1152 · Cited by 468 · see citing works
The design plan of kinesin motors
Vale, R. D., & Fletterick, R. J. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.745 · Cited by 474 · see citing works
Hunter, A., Caplow, M., Coy, D. L., Hancock, W., Diez, S., Wordeman, L., & Howard, J. (2003) · Molecules and Cells · doi:10.1016/s1097-2765(03)00049-2 · Cited by 404 · see citing works
Case, R. B., Pierce, D. W., Hom-Booher, N., Hart, C. L., & Vale, R. D. (1997) · Cell · doi:10.1016/s0092-8674(00)80360-8 · Cited by 348 · see citing works
Chemomechanical coupling of the forward and backward steps of single kinesin molecules
Nishiyama, M., Higuchi, H., & Yanagida, T. (2002) · Nature Cell Biology · doi:10.1038/ncb857 · Cited by 327 · see citing works
Processivity of the Motor Protein Kinesin Requires Two Heads
Hancock, W. O., & Howard, J. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.6.1395 · Cited by 296 · see citing works
Engineering the Processive Run Length of the Kinesin Motor
Thorn, K. S., Ubersax, J. A., & Vale, R. D. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.5.1093 · Cited by 275 · see citing works
Light Chain– dependent Regulation of Kinesin's Interaction with Microtubules
Verhey, K. J., Lizotte, D. L., Abramson, T., Barenboim, L., Schnapp, B. J., & Rapoport, T. A. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.4.1053 · Cited by 266 · see citing works
“This value agrees with the apparent binding constant for MTs based on activation of the ATPase activity of purified dimeric kinesin constructs ( Hackney, 1994, 1995; Rosenfeld et al., 1996; Jiang et al., 1997).”
BackgroundValentine, M. T., Fordyce, P. M., Krzysiak, T. C., Gilbert, S. P., & Block, S. M. (2006) · Nature Cell Biology · doi:10.1038/ncb1394 · Cited by 260 · see citing works
Switches, latches, and amplifiers: common themes of G proteins and molecular motors.
Vale, R. D. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.135.2.291 · Cited by 258 · see citing works
von Delius, M., & Leigh, D. A. (2011) · Chemical Society Reviews · doi:10.1039/c1cs15005g · Cited by 249 · see citing works
Hancock, W. O., & Howard, J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.23.13147 · Cited by 243 · see citing works
Kinetic Mechanism and Regulation of Myosin VI
De La Cruz, E. M., Ostap, E. M., & Sweeney, H. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104136200 · Cited by 234 · see citing works
The kinetic mechanism of kinesin.
Cross, R. (2004) · TIBS -Trends in Biochemical Sciences. Regular ed · doi:10.1016/j.tibs.2004.04.010 · Cited by 204 · see citing works
Transport of ER vesicles on actin filaments in neurons by myosin V
Tabb, J. S., Molyneaux, B. J., Cohen, D. L., Kuznetsov, S. A., & Langford, G. M. (1998) · Journal of Cell Science · doi:10.1242/jcs.111.21.3221 · Cited by 203 · see citing works
Kinesin’s IAK tail domain inhibits initial microtubule-stimulated ADP release
Hackney, D. D., & Stock, M. F. (2000) · Nature Cell Biology · doi:10.1038/35010525 · Cited by 193 · see citing works
Self-citationDistinguishing Inchworm and Hand-Over-Hand Processive Kinesin Movement by Neck Rotation Measurements
Hua, W., Chung, J., & Gelles, J. (2002) · Science · doi:10.1126/science.1063089 · Cited by 194 · see citing works
Examining kinesin processivity within a general gating framework
Andreasson, J. O. L., Milic, B., Chen, G.-Y., Guydosh, N. R., Hancock, W. O., & Block, S. M. (2015) · eLife · doi:10.7554/elife.07403 · Cited by 197 · see citing works
Higher plant myosin XI moves processively on actin with 35 nm steps at high velocity
Tominaga, M., Kojima, H., Yokota, E., Orii, H., Nakamori, R., Katayama, E., Anson, M., Shimmen, T., & Oiwa, K. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg130 · Cited by 186 · see citing works
Transport of Beads by Several Kinesin Motors
Beeg, J., Klumpp, S., Dimova, R., Gracià, R. S., Unger, E., & Lipowsky, R. (2007) · Biophysical Journal · doi:10.1529/biophysj.106.097881 · Cited by 176 · see citing works
Alternating Site Mechanism of the Kinesin ATPase
Gilbert, S. P., Moyer, M. L., & Johnson, K. A. (1998) · Biochemistry · doi:10.1021/bi971117b · Cited by 172 · see citing works
Models of Motor-Assisted Transport of Intracellular Particles
Smith, D. A., & Simmons, R. M. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)75994-2 · Cited by 173 · see citing works
The C-Terminus of Tubulin Increases Cytoplasmic Dynein and Kinesin Processivity
Wang, Z., & Sheetz, M. P. (2000) · Biophysical Journal · doi:10.1016/s0006-3495(00)76743-9 · Cited by 164 · see citing works
Role of the Kinesin Neck Region in Processive Microtubule-based Motility
Romberg, L., Pierce, D. W., & Vale, R. D. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.6.1407 · Cited by 154 · see citing works
A Kinesin-like Motor Inhibits Microtubule Dynamic Instability
Bringmann, H., Skiniotis, G., Spilker, A. C., Kandels-Lewis, S. E., Vernos, I., & Surrey, T. (2004) · Science · doi:10.1126/science.1094838 · Cited by 152 · see citing works
Substeps within the 8-nm step of the ATPase cycle of single kinesin molecules
Nishiyama, M., Muto, E., Inoue, Y., Yanagida, T., & Higuchi, H. (2001) · Nature Cell Biology · doi:10.1038/35070116 · Cited by 142 · see citing works
Controlling Kinesin by Reversible Disulfide Cross-Linking
Tomishige, M., & Vale, R. D. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.5.1081 · Cited by 141 · see citing works
The Role of the Dynactin Complex in Intracellular Motility
Holleran, E. A., Karki, S., & Holzbaur, E. L. F. (1998) · International review of cytology · doi:10.1016/s0074-7696(08)62168-3 · Cited by 144 · see citing works
Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors
Leduc, C., Ruhnow, F., Howard, J., & Diez, S. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701864104 · Cited by 134 · see citing works
Ganguly, S., Williams, L. S., Palacios, I. M., & Goldstein, R. E. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1203575109 · Cited by 126 · see citing works
Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules.
Hirose, K., Lockhart, A., Cross, R. A., & Amos, L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9539 · Cited by 131 · see citing works
Towards the application of cytoskeletal motor proteins in molecular detection and diagnostic devices
Korten, T., Månsson, A., & Diez, S. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.05.001 · Cited by 130 · see citing works
Organelle transport along microtubules — the role of KIFs
Hirokawa, N. (1996) · Trends in Cell Biology · doi:10.1016/0962-8924(96)10003-9 · Cited by 131 · see citing works
The mechanisms of kinesin motor motility: lessons from the monomeric motor KIF1A
Hirokawa, N., Nitta, R., & Okada, Y. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2807 · Cited by 127 · see citing works
Autoinhibition of a Neuronal Kinesin UNC-104/KIF1A Regulates the Size and Density of Synapses
Niwa, S., Lipton, D. M., Morikawa, M., Zhao, C. L., Hirokawa, N., Lu, H., & Shen, K. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.07.043 · Cited by 128 · see citing works
Chiba, K., Takahashi, H., Chen, M., Obinata, H., Arai, S., Hashimoto, K., Oda, T., McKenney, R. J., & Niwa, S. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1905690116 · Cited by 128 · see citing works
Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy.
Coppin, C. M., Finer, J. T., Spudich, J. A., & Vale, R. D. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.5.1913 · Cited by 121 · see citing works
Mavroidis, C., Dubey, A., & Yarmush, M. L. (2004) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.6.040803.140143 · Cited by 118 · see citing works
Dynamic Concentration of Motors in Microtubule Arrays
Nédélec, F., Surrey, T., & Maggs, A. C. (2001) · Physical Review Letters · doi:10.1103/physrevlett.86.3192 · Cited by 112 · see citing works
Kinetic Characterization of a Monomeric Unconventional Myosin V Construct*
Trybus, K., Krementsova, E. B., & Freyzon, Y. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.39.27448 · Cited by 106 · see citing works
Engineering Cooperativity in Biomotor-Protein Assemblies
Diehl, M. R., Zhang, K., Lee, H. J., & Tirrell, D. A. (2006) · Science · doi:10.1126/science.1122125 · Cited by 105 · see citing works
Shekhar, S., Chung, J., Kondev, J., Gelles, J., & Goode, B. L. (2019) · Nature Communications · doi:10.1038/s41467-019-13268-1 · Cited by 100 · see citing works
Distinct conformations of the kinesin Unc104 neck regulate a monomer to dimer motor transition
Al‐Bassam, J., Cui, Y., Klopfenstein, D. R., Carragher, B. O., Vale, R. D., & Milligan, R. A. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200308020 · Cited by 94 · see citing works
Modelling microtubule patterns
Karsenti, E., Nédélec, F., & Surrey, T. (2006) · Nature Cell Biology · doi:10.1038/ncb1498 · Cited by 92 · see citing works
Mehta, A. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.11.1981 · Cited by 90 · see citing works
Lethal Kinesin Mutations Reveal Amino Acids Important for ATPase Activation and Structural Coupling
Brendza, K. M., Rose, D. J., Gilbert, S. P., & Saxton, W. M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.44.31506 · Cited by 85 · see citing works
Demonstration of Coiled-Coil Interactions within the Kinesin Neck Region Using Synthetic Peptides
Tripet, B., Vale, R. D., & Hodges, R. S. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.14.8946 · Cited by 87 · see citing works
Design, Synthesis, and Operation of Small Molecules That Walk along Tracks
von Delius, M., Geertsema, E. M., Leigh, D. A., & Tang, D. D. (2010) · Journal of the American Chemical Society · doi:10.1021/ja106486b · Cited by 84 · see citing works
Structures of kinesin motor proteins
Marx, A., Hoenger, A., & Mandelkow�, E. (2009) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.20392 · Cited by 83 · see citing works
Endow, S. A., Kull, F. J., & Liu, H. (2010) · Journal of Cell Science · doi:10.1242/jcs.064113 · Cited by 79 · see citing works
Kinetic evidence for low chemical processivity in ncd and Eg5
Crevel, I. M.-T. C., Lockhart, A., & Cross, R. A. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1997.1319 · Cited by 83 · see citing works
Trafficking of signaling modules by kinesin motors
Schnapp, B. J. (2003) · Journal of Cell Science · doi:10.1242/jcs.00488 · Cited by 81 · see citing works
Influence of the kinesin neck domain on dimerization and ATPase kinetics
Jiang, W., Stock, M. F., Li, X., & Hackney, D. D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.12.7626 · Cited by 75 · see citing works
Self-citationElevated polar ejection forces stabilize kinetochore–microtubule attachments
Cane, S., Ye, A. A., Luks-Morgan, S. J., & Maresca, T. J. (2013) · The Journal of Cell Biology · doi:10.1083/jcb.201211119 · Cited by 75 · see citing works
KIF1D is a fast non‐processive kinesin that demonstrates novel K‐loop‐dependent mechanochemistry
Rogers, K. R., Weiss, S., Crevel, I., Brophy, P. J., Geeves, M., & Cross, R. (2001) · The EMBO Journal · doi:10.1093/emboj/20.18.5101 · Cited by 73 · see citing works
Plus-end motors override minus-end motors during transport of squid axon vesicles on microtubules.
Mureşan, V., Godek, C. P., Reese, T. S., & Schnapp, B. J. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.135.2.383 · Cited by 72 · see citing works
Kinetics: a tool to study molecular motors.
Gilbert, S., & Mackey, A. T. (2000) · Methods · doi:10.1006/meth.2000.1086 · Cited by 67 · see citing works
Kozielski, F., Arnal, I., & Wade, R. H. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70083-1 · Cited by 68 · see citing works
Gheber, L., Kuo, S. C., & Hoyt, M. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.14.9564 · Cited by 67 · see citing works
Hoenger, A., & Milligan, R. A. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0757 · Cited by 60 · see citing works
The E-Hook of Tubulin Interacts with Kinesin’s Head to Increase Processivity and Speed
Lakämper, S., & Meyhöfer, E. (2005) · Biophysical Journal · doi:10.1529/biophysj.104.057505 · Cited by 59 · see citing works
Motility of single one-headed kinesin molecules along microtubules.
Inoue, Y., Iwane, A., Miyai, T., Muto, E., & Yanagida, T. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)75925-5 · Cited by 57 · see citing works
Kozielski, F., Bonis, S. D., Burmeister, W. P., Cohen‐Addad, C., & Wade, R. H. (1999) · Structure · doi:10.1016/s0969-2126(00)80030-1 · Cited by 58 · see citing works
Motor dynamics underlying cargo transport by pairs of kinesin-1 and kinesin-3 motors
Arpağ, G., Norris, S. R., Mousavi, S. I., Soppina, V., Verhey, K. J., Hancock, W. O., & Tüzel, E. (2019) · Biophysical Journal · doi:10.1016/j.bpj.2019.01.036 · Cited by 56 · see citing works
Kinetics processivity and the direction of motion of Ncd.
Pechatnikova, E., & Taylor, E. W. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)76951-1 · Cited by 57 · see citing works
Regulation of molecular motor proteins
Reilein, A. R., Rogers, S. L., Tuma, M. C., & Gelfand, V. I. (2001) · International review of cytology · doi:10.1016/s0074-7696(01)04005-0 · Cited by 57 · see citing works
Singh, S. K., Pandey, H., Al‐Bassam, J., & Gheber, L. (2018) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-018-2754-7 · Cited by 56 · see citing works
Loop L5 Acts as a Conformational Latch in the Mitotic Kinesin Eg5*
Behnke-Parks, W. M., Vendome, J., Honig, B., Maliga, Z., Moores, C., & Rosenfeld, S. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.192930 · Cited by 63 · see citing works
An ALS ‐associated KIF5A mutant forms oligomers and aggregates and induces neuronal toxicity
Nakano, J., Chiba, K., & Niwa, S. (2022) · Genes to Cells · doi:10.1111/gtc.12936 · Cited by 51 · see citing works
Kinesin velocity increases with the number of motors pulling against viscoelastic drag
Gagliano, J., Walb, M. C., Blaker, B., Macosko, J. C., & Holzwarth, G. (2009) · European Biophysics Journal · doi:10.1007/s00249-009-0560-8 · Cited by 53 · see citing works
Rapid double 8‐nm steps by a kinesin mutant
Higuchi, H., Bronner, C. E., Park, H., & Endow, S. A. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600306 · Cited by 50 · see citing works
KIF14 Binds Tightly to Microtubules and Adopts a Rigor-Like Conformation
Arora, K., Talje, L., Asenjo, A. B., Andersen, P. L., Atchia, K., Joshi, M., Sosa, H., Allingham, J. S., & Kwok, B. H. B. (2014) · Journal of Molecular Biology · doi:10.1016/j.jmb.2014.05.030 · Cited by 49 · see citing works
Diffusion and Directed Movement
Furuta, K., Edamatsu, M., Maeda, Y., & Toyoshima, Y. Y. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m803730200 · Cited by 43 · see citing works
Processive and Nonprocessive Models of Kinesin Movement
Endow, S. A., & Barker, D. S. (2003) · Annual Review of Physiology · doi:10.1146/annurev.physiol.65.092101.142550 · Cited by 47 · see citing works
Importance of a flexible hinge near the motor domain in kinesin‐driven motility
Grummt, M., Woehlke, G., Henningsen, U., Fuchs, S., Schleicher, M., & Schliwa, M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.19.5536 · Cited by 48 · see citing works
Nucleotide-dependent conformations of the kinesin dimer interacting with microtubules
Arnal, I., & Wade, R. H. (1998) · Structure · doi:10.1016/s0969-2126(98)00005-7 · Cited by 45 · see citing works
Single Fungal Kinesin Motor Molecules Move Processively along Microtubules
Lakämper, S., Kallipolitou, A., Woehlke, G., Schliwa, M., & Meyhöfer, E. (2003) · Biophysical Journal · doi:10.1016/s0006-3495(03)74991-1 · Cited by 44 · see citing works
Calcium-induced Mechanical Change in the Neck Domain Alters the Activity of Plant Myosin XI
Tominaga, M., Kojima, H., Yokota, E., Nakamori, R., Anson, M., Shimmen, T., & Oiwa, K. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.346668 · Cited by 44 · see citing works
[18] Studies of molecular motors using caged compounds
Dantzig, J. A., Higuchi, H., & Goldman, Y. E. (1998) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(98)91021-7 · Cited by 43 · see citing works
Unusual properties of the fungal conventional kinesin neck domain from Neurospora crassa
Kallipolitou, A., Deluca, D., Majdic, U., Lakämper, S., Cross, R., Meyhöfer, E., Moroder, L., Schliwa, M., & Woehlke, G. (2001) · The EMBO Journal · doi:10.1093/emboj/20.22.6226 · Cited by 41 · see citing works
Processivity of the Kinesin-2 KIF3A Results from Rear Head Gating and Not Front Head Gating
Chen, G.-Y., Arginteanu, D., & Hancock, W. O. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.628032 · Cited by 40 · see citing works
Monomeric Kinesin Head Domains Hydrolyze Multiple ATP Molecules before Release from a Microtubule
Jiang, W., & Hackney, D. D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.9.5616 · Cited by 41 · see citing works
Self-citationMarcus, A. I., Ambrose, C., Blickley, L., Hancock, W. O., & Cyr, R. J. (2002) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.10045 · Cited by 41 · see citing works
Hirokawa, N. (1996) · Cell Structure and Function · doi:10.1247/csf.21.357 · Cited by 40 · see citing works
Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines
Cochran, J. C. (2015) · Biophysical Reviews · doi:10.1007/s12551-014-0150-6 · Cited by 38 · see citing works
The Loop 5 Element Structurally and Kinetically Coordinates Dimers of the Human Kinesin-5, Eg5
Waitzman, J. S., Larson, A. G., Cochran, J. C., Naber, N. I., Cooke, R., Kull, F. J., Pate, E., & Rice, S. E. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2011.10.032 · Cited by 39 · see citing works
The Second Microtubule-binding Site of Monomeric Kid Enhances the Microtubule Affinity
Shiroguchi, K., Ohsugi, M., Edamatsu, M., Yamamoto, T. S., & Toyoshima, Y. Y. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212274200 · Cited by 36 · see citing works
A kinesin motor in a force-producing conformation
Heuston, E. F., Bronner, C. E., Kull, F. J., & Endow, S. A. (2010) · BMC Structural Biology · doi:10.1186/1472-6807-10-19 · Cited by 35 · see citing works
Alternating Site ATPase Pathway of Rat Conventional Kinesin*
Auerbach, S., & Johnson, K. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m502984200 · Cited by 35 · see citing works
Wang, W., Jiang, Q., Argentini, M., Cornu, D., Gigant, B., Knossow, M., & Wang, C. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.317859 · Cited by 34 · see citing works
The stepping motor protein as a feedback control ratchet
Bier, M. (2006) · Biosystems · doi:10.1016/j.biosystems.2006.07.013 · Cited by 33 · see citing works
Shu, D., & Guo, P. (2003) · Virology · doi:10.1016/s0042-6822(03)00011-4 · Cited by 33 · see citing works
Engineered kinesin motor proteins amenable to small-molecule inhibition
Engelke, M. F., Winding, M., Yue, Y., Shastry, S., Teloni, F., Reddy, S., Blasius, T. L., Soppina, P., Hancock, W. O., Gelfand, V. I., et al. (2016) · Nature Communications · doi:10.1038/ncomms11159 · Cited by 33 · see citing works
- Authors
- Hackney, D. D.
- Affiliations
- Carnegie Mellon University (US)
- Corresponding author
- Hackney, David D.
- ORCID
- Hackney, David D.
- Venue
- Nature
- Published
- 1995-10
- Volume
- 377
- Issue
- 6548
- Pages
- 448-450
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2049953608
- Cited by
- 177
- References
- 13
- Citations by year
- 2026: 4 · 2024: 7 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 2 · 2013: 1 · 2012: 5
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Keywords
- kinesin, microtubule sliding, ATP hydrolysis, processivity, microtubule binding, ADP binding, hand-over-hand mechanism, high-salt conditions, motility assay
- MeSH terms
- Adenosine Diphosphate, Adenosine Triphosphate, Hydrolysis, Microtubules, Kinesins
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