The DOI matches its Crossref record; 114 of the 114 references it cites were found in a registry or an index.
A genome-wide resource for the analysis of protein localisation in Drosophila
Open accessAbstract
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.
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Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Self-citationThe Sequence Alignment/Map format and SAMtools
Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., Marth, G., Abecasis, G., Durbin, R., & 1000 Genome Project Data Processing Subgroup (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp352 · Cited by 62,041
Fast gapped-read alignment with Bowtie 2
Langmead, B., & Salzberg, S. L. (2012) · Nature Methods · doi:10.1038/nmeth.1923 · Cited by 56,400
Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855
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Adams, M. D., Celniker, S. E., Holt, R. A., Evans, C. A., Gocayne, J. D., Amanatides, P. G., Scherer, S. E., Li, P. W., Hoskins, R. A., Galle, R. F., et al. (2000) · Science · doi:10.1126/science.287.5461.2185 · Cited by 5,929
Cox, J., Hein, M. Y., Luber, C. A., Paron, I., Nagaraj, N., & Mann, M. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.031591 · Cited by 5,180
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Gavin, A.-C., Bösche, M., Krause, R., Grandi, P., Marzioch, M., Bauer, A., Schultz, J., Rick, J. M., Michon, A.-M., Cruciat, C.-M., et al. (2002) · Nature · doi:10.1038/415141a · Cited by 4,759
Haplotype-based variant detection from short-read sequencing
Garrison, E., & Marth, G. (2012) · arXiv · doi:10.48550/arxiv.1207.3907 · Cited by 3,700
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
Ho, Y., Gruhler, A., Heilbut, A., Bader, G. D., Moore, L., Adams, S.-L., Millar, A., Taylor, P., Bennett, K., Boutilier, K., et al. (2002) · Nature · doi:10.1038/415180a · Cited by 3,601
Self-citationGlobal landscape of protein complexes in the yeast Saccharomyces cerevisiae
Krogan, N. J., Cagney, G., Yu, H., Zhong, G., Guo, X., Ignatchenko, A., Li, J., Pu, S., Datta, N., Tikuisis, A. P., et al. (2006) · Nature · doi:10.1038/nature04670 · Cited by 2,952
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Anderson, E. N., Gochenaur, L., Singh, A., Grant, R., Patel, K., Watkins, S., Wu, J. Y., & Pandey, U. B. (2018) · Human Molecular Genetics · doi:10.1093/hmg/ddy047 · Cited by 96
Avellaneda, J., Rodier, C., Daian, F., Brouilly, N., Rival, T., Luis, N. M., & Schnorrer, F. (2021) · Nature Communications · doi:10.1038/s41467-021-22058-7 · Cited by 92
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Metadata
- Authors
- Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., KJ, V. V., Krishnan, R., Krishnamoorthy, A., Ferreira, I. R., Ejsmont, R. K., Finkl, K., Hasse, S., Kämpfer, P., Plewka, N., Vinis, E., Schloissnig, S., Knust, E., Hartenstein, V., Mann, M., Ramaswami, M., VijayRaghavan, K., Tomancak, P., & Schnorrer, F.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2016-02-20
- Volume
- 5
- Pages
- e12068-e12068
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2203265148
- Cited by
- 359
- References
- 114
- FWCI
- 18.06
- Type
- journal article
- Language
- en
- Open access
- gold
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- Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., KJ, V. V., Krishnan, R., Krishnamoorthy, A., Ferreira, I. R., Ejsmont, R. K., Finkl, K., Hasse, S., Kämpfer, P., Plewka, N., Vinis, E., Schloissnig, S., . . . Schnorrer, F. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila. eLife. https://doi.org/10.7554/elife.12068
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