The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump
Open accessAbstract
Bacterial efflux pumps confer multidrug resistance by transporting diverse antibiotics from the cell. In Gram-negative bacteria, some of these pumps form multi-protein assemblies that span the cell envelope. Here, we report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that allosterically couples and synchronizes initial ligand binding with channel opening. Within the transport-activated state, the channel remains open even though the pump cycles through three distinct conformations. Collectively, our data provide a dynamic mechanism for the assembly and operation of the AcrAB-TolC pump.
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MolProbity : all-atom structure validation for macromolecular crystallography
Chen, V. B., Arendall, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S., & Richardson, D. C. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909042073 · Cited by 14,115
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
Scheres, S. H. W. (2012) · Journal of Structural Biology · doi:10.1016/j.jsb.2012.09.006 · Cited by 5,446
Rosenthal, P. B., & Henderson, R. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.07.013 · Cited by 2,416
Cited by
Molecular mechanisms of antibiotic resistance revisited
Darby, E. M., Trampari, E., Siasat, P., Gaya, M. S., Alav, I., Webber, M. A., & Blair, J. M. A. (2023) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00820-y · Cited by 1,398
Spatial arrangement of proteins in planar and curved membranes by PPM 3.0
Lomize, A. L., Todd, S. C., & Pogozheva, I. D. (2022) · Protein Science · doi:10.1002/pro.4219 · Cited by 339
Mapping allosteric communications within individual proteins
Wang, J., Jain, A., McDonald, L. R., Gambogi, C., Lee, A. L., & Dokholyan, N. V. (2020) · Nature Communications · doi:10.1038/s41467-020-17618-2 · Cited by 201
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry
Chorev, D. S., Baker, L. A., Wu, D., Beilsten-Edmands, V., Rouse, S. L., Zeev-Ben-Mordehai, T., Jiko, C., Samsudin, F., Gerle, C., Khalid, S., et al. (2018) · Science · doi:10.1126/science.aau0976 · Cited by 191
Gray, T., Storz, G., & Papenfort, K. (2022) · Journal of Bacteriology · doi:10.1128/jb.00341-21 · Cited by 71
Metadata
- Authors
- Wang, Z., Fan, G., Hryc, C. F., Blaza, J. N., Serysheva, I. I., Schmid, M. F., Chiu, W., Luisi, B. F., & Du, D.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2017-03-29
- Volume
- 6
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W2597700712
- Cited by
- 275
- References
- 3
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wang, Z., Fan, G., Hryc, C. F., Blaza, J. N., Serysheva, I. I., Schmid, M. F., Chiu, W., Luisi, B. F., & Du, D. (2017). An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump. eLife. https://doi.org/10.7554/elife.24905
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