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Cited by 93Neurobiology and Insect Physiology ResearchCircadian rhythm and melatoninSleep and Wakefulness Research

Differential regulation of the Drosophila sleep homeostat by circadian and arousal inputs

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Ni, Jinfei D, Gurav, Adishthi S, Liu, Weiwei, Ogunmowo, Tyler H, Hackbart, Hannah, Elsheikh, Ahmed, Verdegaal, Andrew A, Montell, Craig·2019·eLife
DOI 10.7554/elife.40487PMC PMC6363385
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Abstract

One output arm of the sleep homeostat in Drosophila appears to be a group of neurons with projections to the dorsal fan-shaped body (dFB neurons) of the central complex in the brain. However, neurons that regulate the sleep homeostat remain poorly understood. Using neurogenetic approaches combined with Ca2+ imaging, we characterized synaptic connections between dFB neurons and distinct sets of upstream sleep-regulatory neurons. One group of the sleep-promoting upstream neurons is a set of circadian pacemaker neurons that activates dFB neurons via direct glutaminergic excitatory synaptic connections. Opposing this population, a group of arousal-promoting neurons downregulates dFB axonal output with dopamine. Co-activating these two inputs leads to frequent shifts between sleep and wake states. We also show that dFB neurons release the neurotransmitter GABA and inhibit octopaminergic arousal neurons. We propose that dFB neurons integrate synaptic inputs from distinct sets of upstream sleep-promoting circadian clock neurons, and arousal neurons.

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Authors
Ni, J. D., Gurav, A. S., Liu, W., Ogunmowo, T. H., Hackbart, H., Elsheikh, A., Verdegaal, A. A., & Montell, C.
Affiliations
  • Johns Hopkins University (US)
  • University of California, Santa Barbara (US)
Corresponding author
Montell, Craig
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2019-02-05
Volume
8
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.40487
PMC
PMC6363385
OpenAlex
W2911946763
Cited by
93
References
58
Citations by year
2026: 9 · 2025: 11 · 2024: 13 · 2023: 17 · 2022: 16 · 2021: 11 · 2020: 13 · 2019: 3
FWCI
5.14
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
Funders
  • National Institute on Deafness and Other Communication Disorders (DC007864)
  • National Eye Institute (EY008117)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
Keywords
circadian pacemaker neurons, sleep-wake regulation, dopamine, GABA release, octopaminergic neurons, central complex, Drosophila
MeSH terms
Animals, Arousal, Brain, Circadian Rhythm, Drosophila, Nerve Net, Sleep

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