The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
Open accessAbstract
Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish are an attractive model to investigate genetic contributions to neurological diseases. However, current CRISPR-Cas9 methods are difficult to apply to large genetic screens studying behavioural phenotypes. To facilitate rapid genetic screening, we developed a simple sequencing-free tool to validate gRNAs and a highly effective CRISPR-Cas9 method capable of converting >90% of injected embryos directly into F0 biallelic knockouts. We demonstrate that F0 knockouts reliably recapitulate complex mutant phenotypes, such as altered molecular rhythms of the circadian clock, escape responses to irritants, and multi-parameter day-night locomotor behaviours. The technique is sufficiently robust to knockout multiple genes in the same animal, for example to create the transparent triple knockout crystal fish for imaging. Our F0 knockout method cuts the experimental time from gene to behavioural phenotype in zebrafish from months to one week.
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Cites
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No evidence that carotenoid pigments boost either immune or antioxidant defenses in a songbird
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Cited by
Penalva-Tena, A., Bedke, J., Gaudin, A., Barrios, J. P., Bertram, E. P. L., & Douglass, A. D. (2018) · doi:10.1101/444026 · Cited by 41
Quick, R. E., Buck, L. D., Parab, S., Tolbert, Z. R., & Matsuoka, R. L. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.735598 · Cited by 32
Genetic control over biogenic crystal morphogenesis in zebrafish
Deis, R., Lerer-Goldshtein, T., Baiko, O., Eyal, Z., Brenman-Begin, D., Goldsmith, M., Kaufmann, S., Heinig, U., Dong, Y., Lushchekina, S., et al. (2025) · Nature Chemical Biology · doi:10.1038/s41589-024-01722-1 · Cited by 24
SNX10 functions as a modulator of piecemeal mitophagy and mitochondrial bioenergetics
Trachsel-Moncho, L., Veroni, C., Mathai, B. J., Lapao, A., Singh, S., Asp, N. T., Schultz, S. W., Pankiv, S., & Simonsen, A. (2025) · Journal of Cell Biology · doi:10.1083/jcb.202404009 · Cited by 16
ROS signaling–induced mitochondrial Sgk1 expression regulates epithelial cell renewal
Li, Y., Liu, C., Rolling, L., Sikora, V., Chen, Z., Gurwin, J., Barabell, C., Lin, J., & Duan, C. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2216310120 · Cited by 16
Metadata
- Authors
- Kroll, F., Powell, G. T., Ghosh, M., Gestri, G., Antinucci, P., Hearn, T. J., Tunbak, H., Lim, S., Dennis, H. W., Fernandez, J. M., Whitmore, D., Dreosti, E., Wilson, S. W., Hoffman, E. J., & Rihel, J.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2021-01-08
- Volume
- 10
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W3120305566
- Cited by
- 347
- References
- 21
- FWCI
- 18.18
- Type
- journal article
- Language
- en
- Open access
- gold
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- Kroll, F., Powell, G. T., Ghosh, M., Gestri, G., Antinucci, P., Hearn, T. J., Tunbak, H., Lim, S., Dennis, H. W., Fernandez, J. M., Whitmore, D., Dreosti, E., Wilson, S. W., Hoffman, E. J., & Rihel, J. (2021). A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes. eLife. https://doi.org/10.7554/elife.59683
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