The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Assembly of recombinant tau into filaments identical to those of Alzheimer’s disease and chronic traumatic encephalopathy
Open accessAbstract
Abundant filamentous inclusions of tau are characteristic of more than 20 neurodegenerative diseases that are collectively termed tauopathies. Electron cryo-microscopy (cryo-EM) structures of tau amyloid filaments from human brain revealed that distinct tau folds characterise many different diseases. A lack of laboratory-based model systems to generate these structures has hampered efforts to uncover the molecular mechanisms that underlie tauopathies. Here, we report in vitro assembly conditions with recombinant tau that replicate the structures of filaments from both Alzheimer’s disease (AD) and chronic traumatic encephalopathy (CTE), as determined by cryo-EM. Our results suggest that post-translational modifications of tau modulate filament assembly, and that previously observed additional densities in AD and CTE filaments may arise from the presence of inorganic salts, like phosphates and sodium chloride. In vitro assembly of tau into disease-relevant filaments will facilitate studies to determine their roles in different diseases, as well as the development of compounds that specifically bind to these structures or prevent their formation.
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Citations
Cites
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BackgroundSelf-citationA68: a Major Subunit of Paired Helical Filaments and Derivatized Forms of Normal Tau
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Self-citationStructure-based classification of tauopathies
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AlphaFold 2 and NMR Spectroscopy: Partners to Understand Protein Structure, Dynamics and Function
Laurents, D. V. (2022) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2022.906437 · Cited by 95
GSK3β phosphorylation catalyzes the aggregation of tau into Alzheimer’s disease-like filaments
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Disease-associated patterns of acetylation stabilize tau fibril formation
Li, L., Nguyen, B. A., Mullapudi, V., Li, Y., Saelices, L., & Joachimiak, L. A. (2023) · Structure · doi:10.1016/j.str.2023.05.020 · Cited by 25
Tau oligomerization on microtubules in health and disease
Ori‐McKenney, K. M., & McKenney, R. J. (2024) · Cytoskeleton · doi:10.1002/cm.21785 · Cited by 8
Saha, D., Sun, X., Yang, W., Luo, J., & Zheng, W. (2026) · JACS Au · doi:10.1021/jacsau.6c00126 · Cited by 2
Metadata
- Authors
- Lövestam, S., Koh, F. A., van Knippenberg, B., Kotecha, A., Murzin, A. G., Goedert, M., & Scheres, S. H.
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2022-03-04
- Volume
- 11
- ISSN
- 2050-084X (ISSN-L)
- OpenAlex
- W4220728984
- Cited by
- 298
- References
- 15
- FWCI
- 31.06
- Type
- journal article
- Language
- en
- Open access
- gold
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