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Cited by 7Heat shock proteins researchReceptor Mechanisms and SignalingProtein Structure and Dynamics

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Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanism

Open access
Caveney, Nathanael A, Tsutsumi, Naotaka, Garcia, K Christopher·2023·eLife
DOI 10.7554/elife.86784.3

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Abstract

Membrane receptor guanylyl cyclases play a role in many important facets of human physiology, from regulating blood pressure to intestinal fluid secretion. The structural mechanisms which influence these important physiological processes have yet to be explored. We present the 3.9 Å resolution cryo-EM structure of the human membrane receptor guanylyl cyclase GC-C in complex with Hsp90 and its co-chaperone Cdc37, providing insight into the mechanism of Cdc37 mediated binding of GC-C to the Hsp90 regulatory complex. As a membrane protein and non-kinase client of Hsp90–Cdc37, this work shows the remarkable plasticity of Cdc37 to interact with a broad array of clients with significant sequence variation. Furthermore, this work shows how membrane receptor guanylyl cyclases hijack the regulatory mechanisms used for active kinases to facilitate their regulation. Given the known druggability of Hsp90, these insights can guide the further development of membrane receptor guanylyl cyclase-targeted therapeutics and lead to new avenues to treat hypertension, inflammatory bowel disease, and other membrane receptor guanylyl cyclase-related conditions.

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Metadata

Authors
Caveney, N. A., Tsutsumi, N., & Garcia, K. C.
Affiliations
  • Stanford Medicine (US)
  • Howard Hughes Medical Institute (US)
  • Departments of Molecular and Cellular Physiology, and Structural Biology, Stanford University School of Medicine
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2023-08-03
Volume
12
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.86784.3
OpenAlex
W4385546750
Cited by
7
References
3
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 1
FWCI
0.62
Type
journal article
Language
en
Open access
Licence
Locations
eLife (publishedVersion, cc-by)
Funders
  • Canadian Institutes of Health Research (Postdoctoral Fellowship)
  • National Institutes of Health (R01-AI51321)
  • Mathers Foundation
  • Ludwig Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
guanylyl cyclase C, HSP90, Cdc37, receptor structure, hypertension, inflammatory bowel disease

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