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Cited by 7Polysaccharides and Plant Cell WallsPlant Molecular Biology ResearchPlant Gene Expression Analysis

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Cell-type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis

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Kairouani, Alicia, Pontier, Dominique, Picart, Claire, Mounet, Fabien, Martinez, Yves, Le-Bot, Lucie, Fanuel, Mathieu, Hammann, Philippe, Belmudes, Lucid, Merret, Remy, Azevedo, Jacinthe, Carpentier, Marie-Christine, Gagliardi, Dominique, Couté, Yohann, Sibout, Richard, Bies-Etheve, Natacha, Lagrange, Thierry·2023·eLife
DOI 10.7554/elife.88207PMC PMC10541177

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Abstract

Deciphering the mechanism of secondary cell wall/SCW formation in plants is key to understanding their development and the molecular basis of biomass recalcitrance. Although transcriptional regulation is essential for SCW formation, little is known about the implication of post-transcriptional mechanisms in this process. Here we report that two bonafide RNA-binding proteins homologous to the animal translational regulator Musashi, MSIL2 and MSIL4, function redundantly to control SCW formation in Arabidopsis . MSIL2/4 interactomes are similar and enriched in proteins involved in mRNA binding and translational regulation. MSIL2/4 mutations alter SCW formation in the fibers, leading to a reduction in lignin deposition, and an increase of 4- O -glucuronoxylan methylation. In accordance, quantitative proteomics of stems reveal an overaccumulation of glucuronoxylan biosynthetic machinery, including GXM3, in the msil2/4 mutant stem. We showed that MSIL4 immunoprecipitates GXM mRNAs, suggesting a novel aspect of SCW regulation, linking post-transcriptional control to the regulation of SCW biosynthesis genes.

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Metadata

Authors
Kairouani, A., Pontier, D., Picart, C., Mounet, F., Martinez, Y., Le-Bot, L., Fanuel, M., Hammann, P., Belmudes, L., Merret, R., Azevedo, J., Carpentier, M.-C., Gagliardi, D., Couté, Y., Sibout, R., Bies-Etheve, N., & Lagrange, T.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
  • Laboratoire Génome et Développement des Plantes, Université de Perpignan via Domitia, CNRS, UMR5096
Corresponding author
Lagrange, Thierry
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2023-09-29
Volume
12
Pages
RP88207
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.88207
PMC
PMC10541177
OpenAlex
W4380482492
Cited by
7
References
1
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 1
FWCI
1.10
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Agence Nationale de la Recherche (ANR-08-BLAN-0206, ANR-18-EURE-0019, Proteomics French Infrastructure, ANR-17-EURE-0003, ANR-10-INBS-08, ANR-10-IDEX-0002, 12-BSV6-0010, ANR 20-SFRI-0012, IMCBio ANR-17-EURE-0023)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
Keywords
secondary cell wall formation, RNA-binding proteins, post-transcriptional regulation, lignification, glucuronoxylan, xylan biosynthesis, fiber cell differentiation, Arabidopsis thaliana, protein-protein interaction, stem cell wall
MeSH terms
Cell Wall, Lignin, Protein Processing, Post-Translational, Arabidopsis, Gene Expression Regulation, Plant, Arabidopsis Proteins

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