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Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins

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Oppermann, Johannes, Rozenberg, Andrey, Fabrin, Thomaz, González-Cabrera, Cristian, Parker, Rafael, Béjà, Oded, Prigge, Matthias, Hegemann, Peter·2024·eLife
DOI 10.7554/elife.90100.3

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Abstract

Channelrhodopsins (ChRs) are light-gated ion channels widely used to optically activate or silence selected electrogenic cells, such as individual brain neurons. Here, we describe identifying and characterizing a set of anion-conducting ChRs (ACRs) from diverse taxa and representing various branches of the ChR phylogenetic tree. The Mantoniella squamata ACR (MsACR1) showed high sensitivity to yellow-green light ( λ max at 555 nm) and was further engineered for optogenetic applications. A single amino-acid substitution that mimicked red-light-sensitive rhodopsins like Chrimson shifted the photosensitivity 20 nm toward red light and accelerated photocurrent kinetics. Hence, it was named red and accelerated ACR, raACR. Both wild-type and mutant are capable optical silencers at low light intensities in mouse neurons in vitro and in vivo , while raACR offers a higher temporal resolution.

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Metadata

Authors
Oppermann, J., Rozenberg, A., Fabrin, T., González-Cabrera, C., Parker, R., Béjà, O., Prigge, M., & Hegemann, P.
Affiliations
  • Humboldt-Universität zu Berlin (DE)
  • Technion – Israel Institute of Technology (IL)
  • Leibniz Institute for Neurobiology (DE)
  • Research Group Neuromodulatory Networks, Leibniz Institute for Neurobiology
Corresponding author
  • Oppermann, Johannes
  • Prigge, Matthias
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2024-10-14
Volume
12
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.90100.3
OpenAlex
W4380153716
Cited by
2
References
2
Citations by year
2025: 2
FWCI
0.35
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PUBLISSO (German National Library of Medicine) (submittedVersion, cc-by)
Funders
  • Deutsche Forschungsgemeinschaft (SFB 1078 B2 221545957)
  • Israel Science Foundation (3131/20)
  • Alexander von Humboldt-Stiftung (99999.001756/2014-01)
  • EU Joint Programme – Neurodegenerative Disease Research (JPco-FuND - NiPARK)
  • Leibniz-Gemeinschaft (Program 'BestMinds' MP J28/2017 SheLi)
  • Aligning Science Across Parkinson’s (ASAP-020505)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
Keywords
channelrhodopsin, optogenetic inhibition, red light sensitivity, photocurrent kinetics, neuronal silencing, light-gated ion channels

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