Activity of MukBEF for chromosome management in e. coli and its inhibition by MatP
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Abstract
Structural maintenance of chromosomes (SMC) complexes share conserved structures and serve a common role in maintaining chromosome architecture. In the bacterium Escherichia coli , the SMC complex MukBEF is necessary for rapid growth and the accurate segregation and positioning of the chromosome, although the specific molecular mechanisms involved are still unknown. Here, we used a number of in vivo assays to reveal how MukBEF controls chromosome conformation and how the MatP/ matS system prevents MukBEF activity. Our results indicate that the loading of MukBEF occurs preferentially on newly replicated DNA, at multiple loci on the chromosome where it can promote long-range contacts in cis even though MukBEF can promote long-range contacts in the absence of replication. Using Hi-C and ChIP-seq analyses in strains with rearranged chromosomes, the prevention of MukBEF activity increases with the number of matS sites and this effect likely results from the unloading of MukBEF by MatP. Altogether, our results reveal how MukBEF operates to control chromosome folding and segregation in E. coli .
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In E. coli, the SMC complex MukBEF loads preferentially on newly replicated DNA and is excluded from the Ter region by the MatP/matS system.
“In E. coli, the SMC complex MukBEF loads preferentially on newly replicated DNA and is excluded from the Ter region by the MatP/matS system,82”
From Bringing the genetically minimal cell to life on a computer in 4D (2026) · cites this paper as [82]
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Bringing the genetically minimal cell to life on a computer in 4D
Thornburg, Z. R., Maytin, A., Kwon, J., Brier, T. A., Gilbert, B. R., Fu, E., Gao, Y.-L., Quenneville, J., Wu, T., Li, H., et al. (2026) · Cell · doi:10.1016/j.cell.2026.02.009 · Cited by 44 · see citing works
- Authors
- Seba, M., Boccard, F., & Duigou, S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell
- Corresponding author
- Duigou, Stéphane
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2024-02-05
- Volume
- 12
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC10945525
- OpenAlex
- W4387095935
- Cited by
- 16
- References
- 2
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 4
- Type
- posted content
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Agence Nationale de la Recherche (ANR-CE12-0013-01)
- Fondation ARC pour la Recherche sur le Cancer (PJA 20181208006)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- MukBEF, MatP, mats, structural maintenance of chromosomes, chromosome segregation, chromosome folding, DNA replication, long-range chromatin contacts, Escherichia coli, SMC complex, chromosome organization
- MeSH terms
- Chromosomal Proteins, Non-Histone, Chromosomes, Chromosomes, Bacterial, Escherichia coli, Repressor Proteins, Replication Origin, Chromosome Segregation, Escherichia coli Proteins
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