Sideritis scardica extracts inhibit aggregation and toxicity of amyloid- β in Caenorhabditis elegans used as a model for Alzheimer’s disease
Open accessAbstract
Background Beyond its traditional uses in the Balkan area, Sideritis scardica (known as Greek mountain tea, Lamiaceae) is currently extensively investigated for its pharmacological activity in the central nervous system. Antidepressant, psychostimulating, cognition-enhancing and neuroprotective properties have been described. In this study, we tested hydroalcoholic extracts of S. scardica for their potential to counteract amyloid-β toxicity and aggregation, which plays a crucial role in the pathogenesis of Alzheimer’s disease. Methods For this purpose, we have chosen the nematode Caenorhabditis elegans , which is used as a model organism for neurodegenerative diseases. The concentration of different polyphenols in extracts prepared from water, 20, 40, 50, and 70% ethanol was analysed by HPLC. Additionally, polar and unpolar fractions were prepared from the 40% ethanolic extract and phytochemically analysed. Results Essentially, the contents of all measured constituents increased with the lipophilicity of the extraction solvents. Treatment of transgenic C. elegans strains expressing amyloid-β with the extracts resulted in a reduced number of peptide aggregates in the head region of the worms and alleviated toxicity of amyloid-β, observable through the degree of paralysed animals. The mid-polar extracts (40 and 50% ethanol) turned out be the most active, decreasing the plaque number by 21% and delaying the amyloid-β-induced paralysis by up to 3.5 h. The more lipophilic extract fractions exhibited higher activity than the hydrophilic ones. Discussion Sideritis scardica extracts demonstrated pharmacological activity against characteristics of Alzheimer’s disease also in C. elegans , supporting current efforts to assess its potential for the treatment of cognitive decline. The active principle as well as the mode of action needs to be investigated in more detail.
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Sideritis scardica extracts inhibit aggregation and toxicity of amyloid-beta in Caenorhabditis elegans models of Alzheimer's disease.
“S. scardica extracts, which are abundant in a plethora of flavonoids, have been demonstrated to inhibit aggregation and toxicity of amyloid-beta in Caenorhabditis elegans models of AD [107].”
From The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment (2022) · cites this paper as [107]
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The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment
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“scardica extracts, which are abundant in a plethora of flavonoids, have been demonstrated to inhibit aggregation and toxicity of amyloid-beta in Caenorhabditis elegans models of AD [107].”
Background
- Authors
- Heiner, F., Feistel, B., & Wink, M.
- Affiliations
- Heidelberg University (DE)
- Finzelberg GmbH & Co. KG, Andernach, Germany
- Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany
- Corresponding author
- Heiner, Felix
- Venue
- PeerJ
- Publisher
- PeerJ
- Published
- 2018-04-30
- Volume
- 6
- Pages
- e4683
- ISSN
- 2167-8359 (ISSN-L)
- PMC
- PMC5933321
- OpenAlex
- W2780206205
- Cited by
- 40
- References
- 38
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 5 · 2023: 9 · 2022: 6 · 2021: 6 · 2020: 6 · 2019: 1 · 2018: 1
- FWCI
- 2.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PeerJ (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Complementary and alternative medicine › Ginkgo biloba and Cashew Applications
- Keywords
- Sideritis scardica, amyloid-beta, Alzheimer's disease, amyloid-beta aggregation, neuroprotection, Caenorhabditis elegans, polyphenols, ethanol extraction, lipophilicity, neurodegeneration, paralysis, transgenic nematodes, phytochemistry
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