Proteomics of Nasonia vitripennis and the effects of native Wolbachia infection on N. vitripennis
Open accessAbstract
Background: Nasonia vitripennis, a parasitic wasp, is a good model organism to study developmental and evolutionary genetics and to evaluate the interactions between insect hosts and their symbionts.Wolbachia may be the most prevalent endosymbiont among insect species due to their special ability to improve the fitness of the infected hosts. Transinfection of bacteria or fungi could substantially alter the expression of host immune system components. However, few studies have focused on the effects of native Wolbachia infection. Accordingly, in this study, we evaluated the proteomics of N. vitripennis following Wolbachia infection. Methods: We studied the proteomics of N. vitripennis following native Wolbachia infection and in antibiotic-treated Wolbachia-free samples using isobaric tags for relative and absolute quantification-liquid chromatography tandem mass spectrometry, accompanying with some ecological experiments. Results: In total, 3,096 proteins were found to be associated with a wide range of biological processes, molecular functions, and cellular components. Interestingly, there were few significant changes in immune or reproductive proteins between samples with and without Wolbachia infection. Differentially expressed proteins were involved in the binding process, catalytic activity, and the metabolic process, as confirmed by quantitative reverse transcription polymerase chain reaction. Discussion: Invasion of any pathogen or bacterium within a short time can cause an immunoreaction in the host. Our results implied that during the long process of coexistence, the immune system of the host was not as sensitive as when the symbiont initially infected the host, implying that the organisms had gradually adjusted to cohabitation.
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Native Wolbachia does not affect the induction of host immune pathways.
“In contrast, it has been shown that native Wolbachia does not affect the induction of host immune pathways [17, 39].”
From <i>Dirofilaria</i> and <i>Wolbachia</i> in mosquitoes (Diptera: Culicidae) in central European Russia and on the Black Sea coast (2019) · cites this paper as [39]
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- Authors
- Li, J., Wang, N., Liu, Y., & Qiu, S.
- Affiliations
- Shandong Agricultural University (CN)
- Corresponding author
- Wang, Ningxin
- Venue
- PeerJ
- Publisher
- PeerJ
- Published
- 2018-05-28
- Volume
- 6
- Pages
- e4905
- ISSN
- 2167-8359 (ISSN-L)
- PMC
- PMC5978391
- OpenAlex
- W2806288561
- Cited by
- 8
- References
- 60
- Citations by year
- 2025: 2 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PeerJ (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Shandong Province Postdoctoral Innovation Project (201702044)
- China Postdoctoral Science Foundation (2017M612312)
- Project of Shandong Province Higher Educational Science and Technology Program (J17KA149)
- Provincial Key Research and Development Program of Shandong (2017CXGC0207)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Insect and Arachnid Ecology and Behavior
- Keywords
- Nasonia vitripennis, Wolbachia, endosymbionts, host-symbiont interactions, proteomics, host immune response, antibiotic therapy, parasitoid wasps
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