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Cited by 43Antifungal resistance and susceptibilityBacterial Identification and Susceptibility TestingFungal and yeast genetics research

Comparative evaluation of disc diffusion and E-test with broth micro-dilution in susceptibility testing of amphotericin B, voriconazole and caspofungin against clinical Aspergillus isolates

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Gupta, Prashant·2015·JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH
DOI 10.7860/jcdr/2015/10467.5395PMC PMC4347075
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Abstract

The present study was done to compare Disk Diffusion method of CLSI (M51-A), E-test and the reference gold standard CLSI (M38-A2) broth micro-dilution on Indian isolates of Aspergillus spp. MAterIAls And MethOdsPanel of 44 Aspergillus isolates from clinical samples like sputum, nasal mass, endotracheal aspirate, blood cultures, deep seated pus, brain tissue, pleural and peritoneal fluid,were tested over a period of two years (2011)(2012)(2013) in the Department of Microbiology, King George's Medical University, Lucknow, U.P, India.These isolates comprised of 34 A.fumigatus, 8 A. flavus, 2 A.terreus.

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  • E-test results significantly correlate with MIC values obtained through broth or agar dilution methods.

    “studies have shown a significant correlation between E-test results and MIC values obtained through broth or agar dilution methods [69, 70].”

    From Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations (2024) · cites this paper as [70]

    The 'Agreement between CLSI broth-micro dilution, E-test and CLSI disk diffusion' section (page not specified) reports 75–100% agreement between E-test results and broth micro-dilution results for the clinical Aspergillus isolates tested. It does not report a statistically significant correlation between MIC values or compare E-test with agar dilution; its comparison is narrower than the cited claim. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • Previous studies have shown a good correlation between MIC values determined by Etest and those obtained by broth dilution or agar dilution.

    “Several previous studies have shown a good correlation between the MIC values determined by Etest and those obtained by broth dilution or agar dilution method [25], [26], [27].”

    From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [27]

    The Agreement section (page not specified) reports 75–100% agreement in susceptibility results between E-test and broth micro-dilution for clinical Aspergillus isolates, supporting the broth-comparison part broadly. It does not establish a correlation between the numerical MIC values or compare E-test with agar dilution. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 24

  1. naMe, aDDreSS, e-MaiL iD oF the CorreSPonDinG author: Dr. Prashant Gupta, Assistant Professor, Department of Microbiology, King George's Medical University, Lucknow-226003, U.P, India. E-mail : prashantgupta46@hotmail.com FinanCiaL or other CoMPetinG intereStS: None. Date of Submission: jun 30, 2014 Date of Peer Review: Sep 28, 2014 Date of Acceptance: oct 31, 2014 Date of Publishing: jan 01, 2015
  2. Gavalda J, Meije Y, Fortun J, Roilides E, Saliba F, Lortholary O, et al. Invasive [1] fungal infections in solid organ transplant recipients. ClinMicrobiol Infect. 2014;20:27-48.
  3. Xess I, Mohanty S, Jain N, Banerjee U. Prevalence of [2] Aspergillus species in clinical samples isolated in an Indian tertiary care hospital. Indian J Med Sci. 2004;58:513-19.
  4. Munoz P, Guinea J, Bouza E. Treatment options in emerging mold infections. [3] Curr Infect Dis Rep. 2008;10:473-79.
  5. Shivprakash MR, Geertsen E, Chakrabarti A, Mouton JW, Meis JF. In vitro [4] susceptibility of 188 clinical and environmental isolates of Aspergillusflavus for the new triazoleisavuconazole and seven other antifungal drugs. Mycoses. 2011;54:583-89. CLSI. Reference method for broth dilution antifungal susceptibility testing of
  6. filamentous fungi; Approved standard-Second edition. CLSI document M38- A2. Wayne, PA: Clinical and Laboratory Standards Institute; 2008.
  7. Pharmacotherapy. 2001; 21:111S-123S. CLSI. Method for antifungal disk diffusion susceptibility testing of non- [7] dermatophyte filamentous fungi; Approved Guideline, CLSI document M51-A. Wayne, PA: Clinical and Laboratory Standards Institute; 2010.
  8. 8Multicentre evaluation of a new disk agar diffusion method of susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin B, and caspofungin. J ClinMicrobiol. 2007;45:1811-20.
  9. Arikan S, Paetznick V, Rex JH. Comparative evaluation of disk diffusion with [9] micro-dilution assay in susceptibility testing of caspofungin against Aspergillus and Fusariumisolates. Antimicrob Agents Chemother. 2002;46:3084-87.
  10. Espinel-Ingroff A, Diekema DJ, Fothergill A, Johnson E, Pelaez T, Pfaller MA, et [10] al. Wild-Type MIC distributions and epidemiological cutoff values for the triazoles and six Aspergillusspp. for the CLSI broth micro-dilution method (M38-A2). J Clin Microbiol. 2010;48:3251-57.
  11. Espinel-Ingroff A, Fothergill A, Fuller J, Johnson E, Pelaez T, Turnidge J. Wild- [11] Type MIC distributions and epidemiological cutoff values for caspofungin and six Aspergillusspp. for the CLSI broth micro-dilution method (M38-A2). Antimicrob Agents Chemother. 2011;55:2855-59.
  12. Espinel-Ingroff A, Cuenca-Estrella M, Fothergill A, Fuller J, Ghannoum M, [12] Johnson E, et al. Wild-Type MIC distributions and epidemiological cutoff values for amphotericin B and Aspergillus spp. for the CLSI broth microdilution method (M38-A2). Antimicrob Agents Chemother. 2011; 55: 5150-54.
  13. Aspergillus spp. to the new triazolesvoriconazole and posaconazole and to established antifungal agents: Comparison with NCCLS broth micro-dilution method. J Clin Microbiol. 2002; 40:2101-07.
  14. Shivaprakash MR, Chakrabarti A, Geertsen E, Mouton JW, Meis JF. In vitro [14] activity of isavuconazole against 208 Aspergillusflavus isolates in comparison with 7 other antifungal agents: assessment according to the methodology of the European Committee on Antimicrobial Susceptibility Testing. Diag Microbiol Infect Dis. 2011; doi:10.1016/j.diagmicrobio.2011.08.006. · DOI
  15. Espinel-Ingroff A, Johnson E, Hockey H, Troke P. Activities of voriconazole, [15] itraconazole and amphotericin B in vitro against 590 moulds from 323 patients in the voriconazole Phase III clinical studies. J Antimicrob Chemother. 2008;61:616- 20.
  16. Sterling TR, Merz WG. Resistance to amphotericin B: emerging clinical and [16] microbiological pattern. Drug Resist Update. 1998;1:161-65.
  17. Chamilos G, Kontoyiannis DP. Update on Antifungal drug resistance mechanism [17] of Aspergillusfumigatus. Drug Resist Update. 2005;8:344-58.
  18. Blum G, Perkhofer S, Haas H, Schrettl M, Wu¨rzner R, Dierich MP, et al. Potential [18] basis for amphotericin B resistance in Aspergillusterreus. Antimicrob Agents Chemother. 2008; 52:1553-55.
  19. Gomez-Lopez A, Garcia-Effron G, Mellado E, Monzon A, Rodriguez-Tudela [19JL, Cuenca-Estrella M.In vitro activities of three licensed antifungal agents againstSpanish clinical isolates of Aspergillus spp. Antimicrob Agents Chemother. 2003;47:3085-88.
  20. Kaufman D, Boyle R, Hazen KC. Sensitivities of fungal isolates in high-risk [20] preterm infants exposed to fluconazole prophylaxis in a neonatal intensive care unit over a 5-year period. Program and abstracts of the Interscience Conference on Antimicrobial Agents and Chemotherapy; October 30-November 2, 2004;
  21. Alcazar-Fuoli L, Mellado E. Current status of antifungal resistance and its impact [21] on clinical practice. Br J Haematol. 2014;166:471-84. doi: 10.1111/bjh.12896. · DOI
  22. Liu W, Sun Y, Chen W, Liu W, Wan Z, Bu D, et al. T778G mutation in cyp51C [22] gene confers voriconazole-resistance in Aspergillusflavus causing aspergillosis. Antimicrob Agents Chemother. 2012;56:2598-603.doi:10.1128/AAC.05477- 11. · DOI
  23. Arendrup MC, Perkhofer S, Howard SJ, Garcia-Effron G, Vishukumar A, Perlin [23D, et al. Establishing In vitro-In vivo correlations for Aspergillusfumigatus: the challenge of azoles versus echinocandins. Antimicrob Agents Chemother. 2008;52:3504-11.
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Authors
Gupta, P.
Affiliations
King George's Medical University (IN)
Corresponding author
Gupta, Prashant
ORCID
Gupta, Prashant
Venue
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH
Publisher
JCDR Research and Publications
Published
2015
ISSN
  • 0973-709X (ISSN-L)
  • 2249-782X
DOI
10.7860/jcdr/2015/10467.5395
PMC
PMC4347075
OpenAlex
W2145542366
Cited by
43
References
0
Citations by year
2025: 4 · 2024: 4 · 2023: 7 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 4 · 2016: 2 · 2015: 4
FWCI
3.63
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
antifungal susceptibility testing, Aspergillus, amphotericin B, voriconazole, caspofungin, disk diffusion, E-test, broth microdilution, Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, clinical isolates

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