Diagnostics and Susceptibility Testing in Aspergillus
Abstract
UNLABELLED: Invasive aspergillosis is a major cause of morbidity and mortality in immunosuppressed patients. Early diagnosis and correct antifungal treatment have a direct impact on patient survival. A number of newer diagnostic procedures have been developed as alternatives to conventional microbiological methods. The detection of fungal components, largely antigens and DNA, are used in clinical laboratories to diagnose invasive aspergillosis. Other rapid diagnostic tests have been recently developed with promising results. However, antifungal resistance is becoming an emerging problem. The detection of this resistance is important to administer the proper antifungal agent. This text reviews the novelties on new diagnostics Aspergillus spp. PROCEDURES: Intrinsic antifungal resistance and mechanisms of secondary resistance to triazoles in A. fumigatus are also reviewed.
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β1,3 glucan is a fungal polysaccharide not specific for Aspergillus.
“The detection of β1,3 glucan, a fungal polysaccharide not specific for Aspergillus, is based on a very different approach (132–134).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [132]
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Self-citationReversed Halo Sign on Computed Tomography: State-of-the-Art Review
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Azole-resistant Aspergillus fumigatus in the environment of northern Italy, May 2011 to June 2012
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Developments in novel breath tests for bacterial and fungal pulmonary infection
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Comparison of CT Features of Aspergillus and Bacterial Pneumonia in Severely Neutropenic Patients
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Chryssanthou, E., & Cuenca‐Estrella, M. (2006) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2006.01419.x · Cited by 56 · see citing works
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Diagnosis of invasive aspergillosis: recent developments and ongoing challenges
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False-Positive Aspergillus Galactomannan Antigen Test Results
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Galactomannan in Bronchoalveolar Lavage for Diagnosing Invasive Fungal Disease
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Self-citationLoeffler, J., Mengoli, C., Springer, J., Bretagne, S., Cuenca‐Estrella, M., Klingspor, L., Lagrou, K., Melchers, W. J. G., Morton, C. O., Barnes, R. A., et al. (2015) · Journal of Clinical Microbiology · doi:10.1128/jcm.00906-15 · Cited by 50 · see citing works
Self-citationHomogenisation of cystic fibrosis sputum by sonication — An essential step for Aspergillus PCR
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Castro, C., Serrano, M. C. L., Flores, B., Espinel‐Ingroff, A., & Martín‐Mazuelos, E. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.9.4358-4360.2004 · Cited by 46 · see citing works
Alastruey‐Izquierdo, A., Mellado, E., & Cuenca‐Estrella, M. (2012) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2012.06822.x · Cited by 44 · see citing works
Self-citationFatal outcome after heart transplantation caused by A spergillus lentulus
Zbinden, A., Imhof, A., Wilhelm, M. J., Ruschitzka, F. T., Wild, P. J., Bloemberg, G. V., & Mueller, N. J. (2012) · Transplant Infectious Disease · doi:10.1111/j.1399-3062.2012.00779.x · Cited by 44 · see citing works
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First Reported Case of Azole-Resistant Aspergillus fumigatus Due to the TR/L98H Mutation in Germany
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Rocchi, S., Daguindau, É., Grenouillet, F., Deconinck, É., Bellanger, A., Garcia‐Hermoso, D., Bretagne, S., Reboux, G., & Millon, L. (2014) · Journal of Clinical Microbiology · doi:10.1128/jcm.03182-13 · Cited by 38 · see citing works
Ayats, J., Martín‐Mazuelos, E., Pemán, J., Quindós, G., Sánchez, F., García‐Rodríguez, J., Guarro, J., Guinea, J., Linares, M. J. P., Pontón, J., et al. (2010) · Enfermedades Infecciosas y Microbiología Clínica · doi:10.1016/j.eimc.2010.08.005 · Cited by 38 · see citing works
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Bernal‐Martínez, L., Gago, S., Buitrago, M. J., Gómez‐López, A., Rodrı́guez-Tudela, J. L., & Cuenca‐Estrella, M. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.00647-11 · Cited by 33 · see citing works
Self-citationKikuchi, K., Watanabe, A., Itô, J., Oku, Y., Wuren, T., Taguchi, H., Yarita, K., Muraosa, Y., Yahiro, M., Yaguchi, T., et al. (2014) · Journal of Infection and Chemotherapy · doi:10.1016/j.jiac.2014.01.003 · Cited by 32 · see citing works
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Self-citationAspergillus alliaceusandAspergillus flavusco-infection in an acute myeloid leukemia patient
Özhak, B., Alastruey‐Izquierdo, A., Saba, R., Öğünç, D., Öngüt, G., Timurağaoğlu, A., Arslan, G., Cuenca‐Estrella, M., & Rodrı́guez-Tudela, J. L. (2010) · Medical Mycology · doi:10.3109/13693781003749418 · Cited by 29 · see citing works
Self-citationDiagnóstico de laboratorio de la enfermedad fúngica invasora
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Self-citationDirected therapy for fungal infections: focus on aspergillosis
Barnes, R. A. (2013) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkt227 · Cited by 12 · see citing works
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Aspergillus fumigatus and Aspergillosis in 2019
Latgé, J.-P., & Chamilos, G. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00140-18 · Cited by 1,400 · see citing works
Treatment of Chronic Pulmonary Aspergillosis: Current Standards and Future Perspectives
Alastruey-Izquierdo, A., Cadranel, J., Flick, H., Godet, C., Hennequin, C., Hoenigl, M., Kosmidis, C., Lange, C., Munteanu, O., Page, I., et al. (2018) · Respiration · doi:10.1159/000489474 · Cited by 126 · see citing works
Self-citationShelton, J., Collins, R., Uzzell, C. B., Alghamdi, A., Dyer, P. S., Singer, A. C., & Fisher, M. C. (2022) · Applied and Environmental Microbiology · doi:10.1128/aem.02061-21 · Cited by 48 · see citing works
Invasive pulmonary aspergillosis: current diagnostic methodologies and a new molecular approach
Moura, S. P. S. P., Cerqueira, L., & Almeida, A. (2018) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-018-3251-5 · Cited by 43 · see citing works
Siopi, M., Pournaras, S., & Meletiadis, J. (2017) · Journal of Clinical Microbiology · doi:10.1128/jcm.00044-17 · Cited by 25 · see citing works
Exhaled Volatile Organic Compounds for Identifying Patients With Chronic Pulmonary Aspergillosis
Li, Z., Zeng, P., Chen, Z., Guan, W., Wang, T., Lin, Y., Li, S., Zhang, Z., Zhan, Y., Wang, M.-D., et al. (2021) · Frontiers in Medicine · doi:10.3389/fmed.2021.720119 · Cited by 18 · see citing works
Bernal‐Martínez, L., Gil, H., Rivero-Menéndez, O., Gago, S., Cuenca‐Estrella, M., Mellado, E., & Alastruey‐Izquierdo, A. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01083-17 · Cited by 18 · see citing works
Self-citationAzole-Resistant Aspergillus fumigatus: What You Need To Know
Lockhart, S. R., Beer, K. D., & Toda, M. (2019) · Clinical Microbiology Newsletter · doi:10.1016/j.clinmicnews.2019.12.003 · Cited by 18 · see citing works
Meletiadis, J., Siopi, M., Kanioura, L., Jørgensen, K. M., Perlin, D. S., Mouton, J. W., & Arendrup, M. C. (2019) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkz154 · Cited by 16 · see citing works
Rahn, S., Schuck, A., Kondakci, M., Haas, R. R., Neuhausen, N., Pfeffer, K., & Henrich, B. (2016) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2016.10.003 · Cited by 16 · see citing works
TREM1 regulates antifungal immune responses in invasive pulmonary aspergillosis
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Self-citationA Low‐Cost Tebuconazole‐Based Screening Test for Azole‐Resistant Aspergillus fumigatus
Brackin, A. P., Shelton, J., Abdolrasouli, A., Fisher, M. C., & Sewell, T. R. (2020) · Current Protocols in Microbiology · doi:10.1002/cpmc.112 · Cited by 7 · see citing works
Bernal‐Martínez, L., Alcázar‐Fuoli, L., Miguel-Revilla, B., Carvalho, A., García, M. S. C., García‐Rodríguez, J., Cunha, C., de la Pedrosa, E. G., & Gómez‐López, A. (2019) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02399-18 · Cited by 7 · see citing works
Self-citationClinical characteristics and outcomes of pleural aspergillosis: a review of 13 cases
Zhang, J., Gao, X., Wu, J., & Chen, J.-J. (2024) · Microbiology Spectrum · doi:10.1128/spectrum.03852-23 · Cited by 6 · see citing works
Fan, Y., Shang, X., Wang, Y., Zhang, Y., Li, X., Yang, K., Lv, H., & Guo, K. (2025) · BMC Infectious Diseases · doi:10.1186/s12879-025-10769-x · Cited by 5 · see citing works
What Is the Target? Clinical Mycology and Diagnostics
Willinger, B. (2018) · Springer eBooks · doi:10.1007/978-3-319-92300-0_1 · Cited by 5 · see citing works
Welcome to the 13th Volume of Future Microbiology
Greenway, A. (2017) · Future Microbiology · doi:10.2217/fmb-2017-0259 · Cited by 3 · see citing works
In vitro susceptibility of Cuban Aspergillus spp. strains of clinical and environmental origin
Juan, J. L. S., Fernández, C. M., Almaguer, M., Lancha, M. R. P., Martínez, G., Martínez, R. E. V., & Zaragozí, M. T. I. (2017) · Biomédica · doi:10.7705/biomedica.v37i4.3447 · Cited by 3 · see citing works
Necrotizing fasciitis resulting in fatal lung aspergillosis: Uncommon pathogenesis. A case report
Mammadli, T., Kim, B., Rennekampff, H., & Pallua, N. (2017) · Journal de Mycologie Médicale · doi:10.1016/j.mycmed.2017.04.007 · Cited by 2 · see citing works
Kim, C., Yoon, S. Y., Lee, M.-Y., Kim, K. H., Lee, N.-S., & Won, J.-H. (2019) · Soonchunhyang Medical Science · doi:10.15746/sms.19.018 · Cited by 1 · see citing works
Feng, Y., Zhang, Z., Fang, W., Wang, S., Zhang, J., Li, D., Liao, W., Li, R., & Shi, D. (2025) · Mycoses · doi:10.1111/myc.70059 · Cited by 1 · see citing works
Alguacil-Cuéllar, L., Hrynzovska, A., & Alastruey‐Izquierdo, A. (2024) · Springer eBooks · doi:10.1007/978-3-031-76524-7_1 · Cited by 1 · see citing works
Self-citationOrgul, S., Bedoya, Á., Pérez, V. F., Mora, D. R., Sabater, A. L., Miller, D., & Holgado, M. (2025) · Medical Microbiology and Immunology · doi:10.1007/s00430-025-00820-8 · Cited by 1 · see citing works
Hu, S., Cheng, S., & Yang, Y. (2021) · Journal of Public Health and Emergency · doi:10.21037/jphe-21-6
Biomarkers in the Diagnosis of Aspergillosis
Agudelo, L. R., Caceres, D. H., Rincón, J. C. A., Jaramillo, J. C., Zuluaga, A., & Rúa-Giraldo, Á. L. (2026) · Journal of Fungi · doi:10.3390/jof12040259
- Authors
- Bernal-Martínez, L., Alastruey-Izquierdo, A., & Cuenca-Estrella, M.
- Affiliations
- Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain
- Venue
- Future Microbiology
- Publisher
- Informa UK Limited
- Published
- 2016-02
- Volume
- 11
- Issue
- 2
- Pages
- 315-328
- ISSN
- 1746-0913 (ISSN-L)
- 1746-0921
- OpenAlex
- W2270529090
- Cited by
- 25
- References
- 139
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 5 · 2016: 1
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Future Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Spanish Network for Research in Infectious Diseases (RD12/0015)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- invasive aspergillosis, Aspergillus, antifungal susceptibility testing, antifungal resistance, Aspergillus fumigatus, triazole resistance, rapid diagnostic tests, fungal antigens, fungal DNA detection, immunocompromised patients
- MeSH terms
- Antifungal Agents, Aspergillosis, Aspergillus, Humans, Microbial Sensitivity Tests, Microbiological Techniques, Biomarkers, Drug Resistance, Fungal
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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