Joung, J Keith

High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells

Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 3,292

CRISPR-Cas systems for editing, regulating and targeting genomes

Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 3,153

Efficient genome editing in zebrafish using a CRISPR-Cas system

Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology

CRISPR and Genetic EngineeringZebrafish Biomedical Research Applications

Cited by 2,937

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 2,673

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Tsai, S. Q., Zheng, Z., Nguyen, N. T., Liebers, M., Topkar, V. V., Thapar, V., Wyvekens, N., Khayter, C., Iafrate, A. J., Le, L. P., et al. (2015) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 2,268

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M., & Joung, J. K. (2014) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,993

TALENs: A widely applicable technology for targeted genome editing

Joung, J. K., & Sander, J. D. (2013) · Nature Reviews Molecular Cell Biology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,650

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J.-R. J., et al. (2015) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,624

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, J. K., Chen, Z.-Y., & Liu, D. R. (2015) · Nature Biotechnology

RNA Interference and Gene DeliveryCRISPR and Genetic Engineering

Cited by 1,460

Gene therapy comes of age

Dunbar, C. E., High, K. A., Joung, J. K., Kohn, D. B., Ozawa, K., & Sadelain, M. (2018) · Science

Virus-based gene therapy researchCRISPR and Genetic Engineering

Cited by 1,410

CRISPR RNA–guided activation of endogenous human genes

Maeder, M. L., Linder, S. J., Cascio, V. M., Fu, Y., Ho, Q. H., & Joung, J. K. (2013) · Nature Methods

CRISPR and Genetic EngineeringRNA Interference and Gene Delivery

Cited by 1,249

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

Chen, J. S., Dagdas, Y. S., Kleinstiver, B. P., Welch, M. M., Sousa, A. A., Harrington, L. B., Sternberg, S. H., Joung, J. K., Yildiz, A., & Doudna, J. A. (2017) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 1,168

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

Tsai, S. Q., Wyvekens, N., Khayter, C., Foden, J. A., Thapar, V., Reyon, D., Goodwin, M. J., Aryee, M. J., & Joung, J. K. (2014) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 952

CIRCLE-Seq: A highly sensitive in vitro screen for genome-wide CRISPR-cas9 nuclease off-targets

Tsai, S. Q., Nguyen, N. T., Malagon-Lopez, J., Topkar, V. V., Aryee, M. J., & Joung, J. K. (2017) · Nature Methods

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 814

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

Kleinstiver, B. P., Tsai, S. Q., Prew, M. S., Nguyen, N. T., Welch, M. M., Lopez, J. M., McCaw, Z. R., Aryee, M. J., & Joung, J. K. (2016) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 670

Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors

Grünewald, J., Zhou, R., Garcia, S. P., Iyer, S., Lareau, C. A., Aryee, M. J., & Joung, J. K. (2019) · Nature

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 663

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Topkar, V. V., Zheng, Z., & Joung, J. K. (2015) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 611

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald, J., & Joung, J. K. (2021) · Nature Biotechnology

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 573

Adopt a moratorium on heritable genome editing

Lander, E. S., Baylis, F., Zhang, F., Charpentier, E., Berg, P., Bourgain, C., Friedrich, B., Joung, J. K., Li, J., Liu, D., et al. (2019) · Nature

CRISPR and Genetic EngineeringBiomedical Ethics and Regulation

Cited by 512

Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

Pattanayak, V., Ramirez, C. L., Joung, J. K., & Liu, D. R. (2011) · Nature Methods

CRISPR and Genetic EngineeringRNA Interference and Gene Delivery

Cited by 453

Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1

Kiskinis, E., Sandoe, J., Williams, L. A., Boulting, G. L., Moccia, R., Wainger, B. J., Han, S., Peng, T., Thams, S., Mikkilineni, S., et al. (2014) · Cell stem cell

Amyotrophic Lateral Sclerosis ResearchHereditary Neurological Disorders

Cited by 441

High levels of AAV vector integration into CRISPR-induced DNA breaks

Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., et al. (2019) · Nature Communications

Virus-based gene therapy researchCRISPR and Genetic Engineering

Cited by 431

Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs

Listgarten, J., Weinstein, M., Kleinstiver, B. P., Sousa, A. A., Joung, J. K., Crawford, J., Gao, K., Hoang, L., Elibol, M., Doench, J. G., et al. (2018) · Nature Biomedical Engineering

CRISPR and Genetic EngineeringRNA regulation and disease

Cited by 336

TALEN-based Gene Correction for Epidermolysis Bullosa

Osborn, M. J., Starker, C. G., McElroy, A. N., Webber, B. R., Riddle, M. J., Xia, L., DeFeo, A. P., Gabriel, R., Schmidt, M., Von Kalle, C., et al. (2013) · Molecular Therapy

CRISPR and Genetic EngineeringVirus-based gene therapy research

Cited by 258